Restriction map for
pFN21AE6746
(Linear) (Six-base) MAPSORT of: KIEE6746.seq Check: 9224 from: 1 to: 3564
KIEE6746.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1251 3292 3564
Size: 1251 2041 272
Fragments arranged by size:
2041 1251 272
AccI GT'mk_AC
Cuts at: 0 1159 3564
Size: 1159 2405
AclI AA'CG_TT
Cuts at: 0 2727 3564
Size: 2727 837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1733 2326 3564
Size: 1367 366 593 1238
Fragments arranged by size:
1367 1238 593 366
AflII C'TTAA_G
Cuts at: 0 31 3564
Size: 31 3533
AflIII A'CryG_T
Cuts at: 0 679 3564
Size: 679 2885
AhdI GACnn_n'nnGTC
Cuts at: 0 2972 3564
Size: 2972 592
AleI CACnn'nnGTG
Cuts at: 0 361 2358 2501 3564
Size: 361 1997 143 1063
Fragments arranged by size:
1997 1063 361 143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2376 3466 3491 3564
Size: 2376 1090 25 73
Fragments arranged by size:
2376 1090 73 25
AlwNI CAG_nnn'CTG
Cuts at: 0 951 3318 3564
Size: 951 2367 246
Fragments arranged by size:
2367 951 246
ApaI G_GGCC'C
Cuts at: 0 728 1005 3564
Size: 728 277 2559
Fragments arranged by size:
2559 728 277
ApoI r'AATT_y
Cuts at: 0 199 323 891 1702 2007 2253 3073 3564
Size: 199 124 568 811 305 246 820 491
Fragments arranged by size:
820 811 568 491 305 246 199 124
AseI AT'TA_AT
Cuts at: 0 2138 3564
Size: 2138 1426
AvaI C'yCGr_G
Cuts at: 0 125 3332 3564
Size: 125 3207 232
Fragments arranged by size:
3207 232 125
BamHI G'GATC_C
Cuts at: 0 59 3564
Size: 59 3505
BanI G'GyrC_C
Cuts at: 0 156 363 2464 3564
Size: 156 207 2101 1100
Fragments arranged by size:
2101 1100 207 156
BanII G_rGCy'C
Cuts at: 0 728 994 1005 1971 3202 3564
Size: 728 266 11 966 1231 362
Fragments arranged by size:
1231 966 728 362 266 11
BbeI G_GCGC'C
Cuts at: 0 2468 3564
Size: 2468 1096
BbsI GAAGACnn'nnnn_
Cuts at: 0 1373 1614 3424 3564
Size: 1373 241 1810 140
Fragments arranged by size:
1810 1373 241 140
BciVI GTATCCnnnnn_n'
Cuts at: 0 1072 3564
Size: 1072 2492
BclI T'GATC_A
Cuts at: 0 371 2285 3398 3564
Size: 371 1914 1113 166
Fragments arranged by size:
1914 1113 371 166
BglI GCCn_nnn'nGGC
Cuts at: 0 2218 3564
Size: 2218 1346
BglII A'GATC_T
Cuts at: 0 803 3564
Size: 803 2761
Bme1580I G_kGCm'C
Cuts at: 0 728 1005 3564
Size: 728 277 2559
Fragments arranged by size:
2559 728 277
BmgBI CAC'GTC
Cuts at: 0 134 2277 3564
Size: 134 2143 1287
Fragments arranged by size:
2143 1287 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 1191 3564
Size: 1191 2373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 2628 2968 3149 3322 3564
Size: 172 2456 340 181 173 242
Fragments arranged by size:
2456 340 242 181 173 172
Bpu10I CC'TnA_GC
Cuts at: 0 2821 3196 3564
Size: 2821 375 368
Fragments arranged by size:
2821 375 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1148 1165 3470 3564
Size: 895 253 17 2305 94
Fragments arranged by size:
2305 895 253 94 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 1025 3049 3564
Size: 137 888 2024 515
Fragments arranged by size:
2024 888 515 137
BsaAI yAC'GTr
Cuts at: 0 682 2499 3564
Size: 682 1817 1065
Fragments arranged by size:
1817 1065 682
BsaBI GATnn'nnATC
Cuts at: 0 1469 2163 3564
Size: 1469 694 1401
Fragments arranged by size:
1469 1401 694
BsaHI Gr'CG_yC
Cuts at: 0 2465 3421 3461 3564
Size: 2465 956 40 103
Fragments arranged by size:
2465 956 103 40
BsaWI w'CCGG_w
Cuts at: 0 2330 3564
Size: 2330 1234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 509 539 2302 2332 3564
Size: 509 30 1763 30 1232
Fragments arranged by size:
1763 1232 509 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1397 1933 2349 2381 3319 3564
Size: 1397 536 416 32 938 245
Fragments arranged by size:
1397 938 536 416 245 32
BseYI C'CCAG_C
Cuts at: 0 1611 2259 2831 3408 3564
Size: 1611 648 572 577 156
Fragments arranged by size:
1611 648 577 572 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1347 2223 3331 3564
Size: 964 383 876 1108 233
Fragments arranged by size:
1108 964 876 383 233
BsiEI CG_ry'CG
Cuts at: 0 3393 3564
Size: 3393 171
BsiHKAI G_wGCw'C
Cuts at: 0 994 1971 3202 3564
Size: 994 977 1231 362
Fragments arranged by size:
1231 994 977 362
BsmI GAATG_Cn'
Cuts at: 0 1487 3564
Size: 1487 2077
BsmBI CGTCTCn'nnnn_
Cuts at: 0 622 2453 3564
Size: 622 1831 1111
Fragments arranged by size:
1831 1111 622
Bsp1286I G_dGCh'C
Cuts at: 0 728 994 1005 1971 3202 3564
Size: 728 266 11 966 1231 362
Fragments arranged by size:
1231 966 728 362 266 11
BspEI T'CCGG_A
Cuts at: 0 2330 3564
Size: 2330 1234
BspHI T'CATG_A
Cuts at: 0 1310 3564
Size: 1310 2254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1251 3292 3564
Size: 1251 2041 272
Fragments arranged by size:
2041 1251 272
BsrBI CCG'CTC
Cuts at: 0 65 3564
Size: 65 3499
BsrDI GCAATG_nn'
Cuts at: 0 305 599 1331 3158 3295 3372 3564
Size: 305 294 732 1827 137 77 192
Fragments arranged by size:
1827 732 305 294 192 137 77
BsrGI T'GTAC_A
Cuts at: 0 2744 3564
Size: 2744 820
BssSI C'ACGA_G
Cuts at: 0 2358 3564
Size: 2358 1206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 439 3564
Size: 439 3125
BstBI TT'CG_AA
Cuts at: 0 1740 2324 3564
Size: 1740 584 1240
Fragments arranged by size:
1740 1240 584
BstEII G'GTnAC_C
Cuts at: 0 2622 3564
Size: 2622 942
BstYI r'GATC_y
Cuts at: 0 59 803 947 3564
Size: 59 744 144 2617
Fragments arranged by size:
2617 744 144 59
BstZ17I GTA'TAC
Cuts at: 0 1160 3564
Size: 1160 2404
Bsu36I CC'TnA_GG
Cuts at: 0 587 3564
Size: 587 2977
BtgI C'CryG_G
Cuts at: 0 46 71 1223 1859 3564
Size: 46 25 1152 636 1705
Fragments arranged by size:
1705 1152 636 46 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1675 2055 2544 3564
Size: 1675 380 489 1020
Fragments arranged by size:
1675 1020 489 380
BtsI GCAGTG_nn'
Cuts at: 0 518 662 2235 2889 3564
Size: 518 144 1573 654 675
Fragments arranged by size:
1573 675 654 518 144
ClaI AT'CG_AT
Cuts at: 0 2291 3564
Size: 2291 1273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 120 155 3564
Size: 120 35 3409
Fragments arranged by size:
3409 120 35
EaeI y'GGCC_r
Cuts at: 0 224 1226 1511 2295 3564
Size: 224 1002 285 784 1269
Fragments arranged by size:
1269 1002 784 285 224
EarI CTCTTCn'nnn_
Cuts at: 0 273 307 1244 1575 1976 2929 3564
Size: 273 34 937 331 401 953 635
Fragments arranged by size:
953 937 635 401 331 273 34
EcoICRI GAG'CTC
Cuts at: 0 992 1969 3200 3564
Size: 992 977 1231 364
Fragments arranged by size:
1231 992 977 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1367 1733 2326 2628 2968 3149 3322
Size: 172 1195 366 593 302 340 181 173
Cuts at: 3322 3564
Size: 242
Fragments arranged by size:
1195 593 366 340 302 242 181 173
172
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2191 3454 3564
Size: 2191 1263 110
Fragments arranged by size:
2191 1263 110
EcoO109I rG'GnC_Cy
Cuts at: 0 76 724 1001 1002 3564
Size: 76 648 277 1 2562
Fragments arranged by size:
2562 648 277 76 1
EcoRI G'AATT_C
Cuts at: 0 891 3564
Size: 891 2673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1632 2062 2652 3564
Size: 1632 430 590 912
Fragments arranged by size:
1632 912 590 430
HaeII r_GCGC'y
Cuts at: 0 907 2468 3564
Size: 907 1561 1096
Fragments arranged by size:
1561 1096 907
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1048 1080 1392 1424 1538 1570
Size: 106 32 910 32 312 32 114 32
Cuts at: 1570 2958 2990 3564
Size: 1388 32 574
Fragments arranged by size:
1388 910 574 312 114 106 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 242 447 2111 2966 3564
Size: 242 205 1664 855 598
Fragments arranged by size:
1664 855 598 242 205
HindIII A'AGCT_T
Cuts at: 0 2584 3564
Size: 2584 980
HpaI GTT'AAC
Cuts at: 0 447 3564
Size: 447 3117
KasI G'GCGC_C
Cuts at: 0 2464 3564
Size: 2464 1100
MfeI C'AATT_G
Cuts at: 0 386 3564
Size: 386 3178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 364 1378 1958 2979 3564
Size: 120 244 1014 580 1021 585
Fragments arranged by size:
1021 1014 585 580 244 120
MscI TGG'CCA
Cuts at: 0 1228 2297 3564
Size: 1228 1069 1267
Fragments arranged by size:
1267 1228 1069
MslI CAynn'nnrTG
Cuts at: 0 361 740 1192 2358 2501 3473 3564
Size: 361 379 452 1166 143 972 91
Fragments arranged by size:
1166 972 452 379 361 143 91
MspA1I CmG'CkG
Cuts at: 0 48 150 3564
Size: 48 102 3414
Fragments arranged by size:
3414 102 48
NarI GG'CG_CC
Cuts at: 0 2465 3564
Size: 2465 1099
NcoI C'CATG_G
Cuts at: 0 1223 3564
Size: 1223 2341
NdeI CA'TA_TG
Cuts at: 0 2374 3018 3564
Size: 2374 644 546
Fragments arranged by size:
2374 644 546
NspI r_CATG'y
Cuts at: 0 675 1304 2532 2820 3105 3564
Size: 675 629 1228 288 285 459
Fragments arranged by size:
1228 675 629 459 288 285
PflMI CCAn_nnn'nTGG
Cuts at: 0 497 3065 3564
Size: 497 2568 499
Fragments arranged by size:
2568 499 497
PfoI T'CCnGG_A
Cuts at: 0 1697 2342 3553 3564
Size: 1697 645 1211 11
Fragments arranged by size:
1697 1211 645 11
PmlI CAC'GTG
Cuts at: 0 682 2499 3564
Size: 682 1817 1065
Fragments arranged by size:
1817 1065 682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2668 2700 3564
Size: 2668 32 864
Fragments arranged by size:
2668 864 32
PpuMI rG'GwC_Cy
Cuts at: 0 76 3564
Size: 76 3488
PshAI GACnn'nnGTC
Cuts at: 0 1394 3564
Size: 1394 2170
PspOMI G'GGCC_C
Cuts at: 0 724 1001 3564
Size: 724 277 2563
Fragments arranged by size:
2563 724 277
PstI C_TGCA'G
Cuts at: 0 2383 2399 3455 3564
Size: 2383 16 1056 109
Fragments arranged by size:
2383 1056 109 16
PvuII CAG'CTG
Cuts at: 0 150 3564
Size: 150 3414
SacI G_AGCT'C
Cuts at: 0 994 1971 3202 3564
Size: 994 977 1231 362
Fragments arranged by size:
1231 994 977 362
SacII CC_GC'GG
Cuts at: 0 49 3564
Size: 49 3515
SapI GCTCTTCn'nnn_
Cuts at: 0 273 1244 3564
Size: 273 971 2320
Fragments arranged by size:
2320 971 273
SfcI C'TryA_G
Cuts at: 0 710 1413 2379 2395 3451 3564
Size: 710 703 966 16 1056 113
Fragments arranged by size:
1056 966 710 703 113 16
SfoI GGC'GCC
Cuts at: 0 2466 3564
Size: 2466 1098
SmaI CCC'GGG
Cuts at: 0 127 3564
Size: 127 3437
SmlI C'TyrA_G
Cuts at: 0 31 910 1144 1163 3332 3485 3564
Size: 31 879 234 19 2169 153 79
Fragments arranged by size:
2169 879 234 153 79 31 19
SphI G_CATG'C
Cuts at: 0 2532 2820 3105 3564
Size: 2532 288 285 459
Fragments arranged by size:
2532 459 288 285
SspI AAT'ATT
Cuts at: 0 3006 3564
Size: 3006 558
StuI AGG'CCT
Cuts at: 0 17 1567 3564
Size: 17 1550 1997
Fragments arranged by size:
1997 1550 17
StyI C'CwwG_G
Cuts at: 0 789 983 1223 2247 2903 3564
Size: 789 194 240 1024 656 661
Fragments arranged by size:
1024 789 661 656 240 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2094 2491 2863 3564
Size: 2094 397 372 701
Fragments arranged by size:
2094 701 397 372
TatI w'GTAC_w
Cuts at: 0 230 1007 2639 2744 3564
Size: 230 777 1632 105 820
Fragments arranged by size:
1632 820 777 230 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 1515 1547 1866 1898 3335 3367
Size: 509 32 974 32 319 32 1437 32
Cuts at: 3367 3564
Size: 197
Fragments arranged by size:
1437 974 509 319 197 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1131 2943 3564
Size: 1131 1812 621
Fragments arranged by size:
1812 1131 621
XhoI C'TCGA_G
Cuts at: 0 3332 3564
Size: 3332 232
XmaI C'CCGG_G
Cuts at: 0 125 3564
Size: 125 3439
XmnI GAAnn'nnTTC
Cuts at: 0 1746 3564
Size: 1746 1818
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I
NarI NcoI NdeI NspI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AloI ApaLI AscI AsiSI
AvrII BaeI BbvCI BcgI BfrBI BlpI BmtI BplI
BsiWI BsrFI BssHII BstXI DraI DraIII DrdI EagI
EciI EcoRV FseI FspI FspAI KpnI MluI NaeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI Tth111I XbaI ZraI