(Linear) (Six-base) MAPSORT of: KIEE6743.seq Check: 9459 from: 1 to: 1884 KIEE6743.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 136 259 1594 1884 Size: 136 123 1335 290 Fragments arranged by size: 1335 290 136 123 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 940 946 1136 1644 1794 1884 Size: 847 93 6 190 508 150 90 Fragments arranged by size: 847 508 190 150 93 90 6 AfeI AGC'GCT Cuts at: 0 1873 1884 Size: 1873 11 AflII C'TTAA_G Cuts at: 0 1002 1884 Size: 1002 882 AleI CACnn'nnGTG Cuts at: 0 1049 1060 1884 Size: 1049 11 824 Fragments arranged by size: 1049 824 11 AlwNI CAG_nnn'CTG Cuts at: 0 898 1281 1461 1884 Size: 898 383 180 423 Fragments arranged by size: 898 423 383 180 ApaLI G'TGCA_C Cuts at: 0 1124 1884 Size: 1124 760 ApoI r'AATT_y Cuts at: 0 506 907 1884 Size: 506 401 977 Fragments arranged by size: 977 506 401 AvaI C'yCGr_G Cuts at: 0 224 796 1884 Size: 224 572 1088 Fragments arranged by size: 1088 572 224 BanI G'GyrC_C Cuts at: 0 809 1780 1884 Size: 809 971 104 Fragments arranged by size: 971 809 104 BanII G_rGCy'C Cuts at: 0 1262 1884 Size: 1262 622 BbeI G_GCGC'C Cuts at: 0 813 1784 1884 Size: 813 971 100 Fragments arranged by size: 971 813 100 BbsI GAAGACnn'nnnn_ Cuts at: 0 857 1884 Size: 857 1027 BbvCI CC'TCA_GC Cuts at: 0 1166 1884 Size: 1166 718 BglI GCCn_nnn'nGGC Cuts at: 0 491 1700 1884 Size: 491 1209 184 Fragments arranged by size: 1209 491 184 BlpI GC'TnA_GC Cuts at: 0 1022 1884 Size: 1022 862 Bme1580I G_kGCm'C Cuts at: 0 712 1128 1884 Size: 712 416 756 Fragments arranged by size: 756 712 416 BmgBI CAC'GTC Cuts at: 0 1488 1884 Size: 1488 396 BmrI ACTGGGnnnn_n' Cuts at: 0 1845 1854 1884 Size: 1845 9 30 Fragments arranged by size: 1845 30 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 676 682 697 1537 1562 1863 1884 Size: 251 425 6 15 840 25 301 21 Fragments arranged by size: 840 425 301 251 25 21 15 6 Bpu10I CC'TnA_GC Cuts at: 0 1166 1677 1884 Size: 1166 511 207 Fragments arranged by size: 1166 511 207 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 1354 1884 Size: 760 594 530 Fragments arranged by size: 760 594 530 BsaI GGTCTCn'nnnn_ Cuts at: 0 72 673 1884 Size: 72 601 1211 Fragments arranged by size: 1211 601 72 BsaAI yAC'GTr Cuts at: 0 1447 1884 Size: 1447 437 BsaHI Gr'CG_yC Cuts at: 0 810 1781 1884 Size: 810 971 103 Fragments arranged by size: 971 810 103 BsaWI w'CCGG_w Cuts at: 0 134 1884 Size: 134 1750 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 27 57 243 273 670 700 1884 Size: 27 30 186 30 397 30 1184 Fragments arranged by size: 1184 397 186 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1540 1631 1884 Size: 1540 91 253 Fragments arranged by size: 1540 253 91 BseYI C'CCAG_C Cuts at: 0 1868 1884 Size: 1868 16 BsiEI CG_ry'CG Cuts at: 0 54 1884 Size: 54 1830 BsiHKAI G_wGCw'C Cuts at: 0 1128 1262 1884 Size: 1128 134 622 Fragments arranged by size: 1128 622 134 BsmI GAATG_Cn' Cuts at: 0 1320 1884 Size: 1320 564 Bsp1286I G_dGCh'C Cuts at: 0 712 1128 1262 1884 Size: 712 416 134 622 Fragments arranged by size: 712 622 416 134 BspEI T'CCGG_A Cuts at: 0 134 1884 Size: 134 1750 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 136 259 701 779 1331 1594 1884 Size: 136 123 442 78 552 263 290 Fragments arranged by size: 552 442 290 263 136 123 78 BsrBI CCG'CTC Cuts at: 0 56 140 296 594 1884 Size: 56 84 156 298 1290 Fragments arranged by size: 1290 298 156 84 56 BsrDI GCAATG_nn' Cuts at: 0 1109 1334 1884 Size: 1109 225 550 Fragments arranged by size: 1109 550 225 BstXI CCAn_nnnn'nTGG Cuts at: 0 207 1884 Size: 207 1677 BtgI C'CryG_G Cuts at: 0 1073 1205 1571 1884 Size: 1073 132 366 313 Fragments arranged by size: 1073 366 313 132 BtsI GCAGTG_nn' Cuts at: 0 1689 1884 Size: 1689 195 EaeI y'GGCC_r Cuts at: 0 51 1331 1692 1817 1884 Size: 51 1280 361 125 67 Fragments arranged by size: 1280 361 125 67 51 EagI C'GGCC_G Cuts at: 0 51 1884 Size: 51 1833 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 131 264 1584 1643 1884 Size: 131 133 1320 59 241 Fragments arranged by size: 1320 241 133 131 59 EcoICRI GAG'CTC Cuts at: 0 1260 1884 Size: 1260 624 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 676 682 697 847 940 946 1136 Size: 251 425 6 15 150 93 6 190 Cuts at: 1136 1537 1562 1644 1794 1863 1884 Size: 401 25 82 150 69 21 Fragments arranged by size: 425 401 251 190 150 150 93 82 69 25 21 15 6 6 EcoO109I rG'GnC_Cy Cuts at: 0 122 840 1007 1170 1884 Size: 122 718 167 163 714 Fragments arranged by size: 718 714 167 163 122 EcoRI G'AATT_C Cuts at: 0 506 1884 Size: 506 1378 EcoRV GAT'ATC Cuts at: 0 1140 1884 Size: 1140 744 HaeII r_GCGC'y Cuts at: 0 813 1784 1875 1884 Size: 813 971 91 9 Fragments arranged by size: 971 813 91 9 KasI G'GCGC_C Cuts at: 0 809 1780 1884 Size: 809 971 104 Fragments arranged by size: 971 809 104 MslI CAynn'nnrTG Cuts at: 0 205 1049 1060 1223 1570 1884 Size: 205 844 11 163 347 314 Fragments arranged by size: 844 347 314 205 163 11 MspA1I CmG'CkG Cuts at: 0 214 257 895 1742 1751 1760 1884 Size: 214 43 638 847 9 9 124 Fragments arranged by size: 847 638 214 124 43 9 9 NarI GG'CG_CC Cuts at: 0 810 1781 1884 Size: 810 971 103 Fragments arranged by size: 971 810 103 NcoI C'CATG_G Cuts at: 0 1073 1884 Size: 1073 811 PasI CC'CwG_GG Cuts at: 0 487 1884 Size: 487 1397 PfoI T'CCnGG_A Cuts at: 0 653 674 1884 Size: 653 21 1210 Fragments arranged by size: 1210 653 21 PpuMI rG'GwC_Cy Cuts at: 0 840 1007 1884 Size: 840 167 877 Fragments arranged by size: 877 840 167 PstI C_TGCA'G Cuts at: 0 542 1202 1328 1403 1884 Size: 542 660 126 75 481 Fragments arranged by size: 660 542 481 126 75 PvuII CAG'CTG Cuts at: 0 214 257 895 1884 Size: 214 43 638 989 Fragments arranged by size: 989 638 214 43 SacI G_AGCT'C Cuts at: 0 1262 1884 Size: 1262 622 SexAI A'CCwGG_T Cuts at: 0 842 1884 Size: 842 1042 SfcI C'TryA_G Cuts at: 0 538 1198 1324 1399 1411 1884 Size: 538 660 126 75 12 473 Fragments arranged by size: 660 538 473 126 75 12 SfoI GGC'GCC Cuts at: 0 811 1782 1884 Size: 811 971 102 Fragments arranged by size: 971 811 102 SmaI CCC'GGG Cuts at: 0 226 1884 Size: 226 1658 SmlI C'TyrA_G Cuts at: 0 739 1002 1369 1884 Size: 739 263 367 515 Fragments arranged by size: 739 515 367 263 StyI C'CwwG_G Cuts at: 0 1073 1884 Size: 1073 811 TatI w'GTAC_w Cuts at: 0 1529 1884 Size: 1529 355 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 75 107 243 275 670 702 1884 Size: 75 32 136 32 395 32 1182 Fragments arranged by size: 1182 395 136 75 32 32 32 XmaI C'CCGG_G Cuts at: 0 224 1884 Size: 224 1660 Enzymes that do cut: AarI AcuI AfeI AflII AleI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BstXI BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII KasI MslI MspA1I NarI NcoI PasI PfoI PpuMI PstI PvuII SacI SexAI SfcI SfoI SmaI SmlI StyI TatI TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflIII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsgI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoNI FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI