Restriction map for
pFN21AE6742
(Linear) (Six-base) MAPSORT of: KIEE6742.seq Check: 3856 from: 1 to: 4281
KIEE6742.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 561 3301 4113 4281
Size: 561 2740 812 168
Fragments arranged by size:
2740 812 561 168
AccI GT'mk_AC
Cuts at: 0 1748 4281
Size: 1748 2533
Acc65I G'GTAC_C
Cuts at: 0 4233 4281
Size: 4233 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 2350 4204 4281
Size: 97 2253 1854 77
Fragments arranged by size:
2253 1854 97 77
AfeI AGC'GCT
Cuts at: 0 304 3820 4281
Size: 304 3516 461
Fragments arranged by size:
3516 461 304
AflIII A'CryG_T
Cuts at: 0 738 1454 2682 2684 3719 4281
Size: 738 716 1228 2 1035 562
Fragments arranged by size:
1228 1035 738 716 562 2
AhdI GACnn_n'nnGTC
Cuts at: 0 4254 4281
Size: 4254 27
AleI CACnn'nnGTG
Cuts at: 0 1409 2193 2685 3042 3805 4281
Size: 1409 784 492 357 763 476
Fragments arranged by size:
1409 784 763 492 476 357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2724 3584 3987 4281
Size: 2724 860 403 294
Fragments arranged by size:
2724 860 403 294
AlwNI CAG_nnn'CTG
Cuts at: 0 409 722 810 1371 2527 2991 3572 4281
Size: 409 313 88 561 1156 464 581 709
Fragments arranged by size:
1156 709 581 561 464 409 313 88
ApaI G_GGCC'C
Cuts at: 0 268 2081 2367 2940 3386 3545 3917 4281
Size: 268 1813 286 573 446 159 372 364
Fragments arranged by size:
1813 573 446 372 364 286 268 159
ApaLI G'TGCA_C
Cuts at: 0 376 4281
Size: 376 3905
AscI GG'CGCG_CC
Cuts at: 0 707 4281
Size: 707 3574
AseI AT'TA_AT
Cuts at: 0 3986 4281
Size: 3986 295
AvaI C'yCGr_G
Cuts at: 0 17 868 1538 2933 3181 3491 3538 3661
Size: 17 851 670 1395 248 310 47 123
Cuts at: 3661 4281
Size: 620
Fragments arranged by size:
1395 851 670 620 310 248 123 47
17
AvrII C'CTAG_G
Cuts at: 0 3040 4281
Size: 3040 1241
BamHI G'GATC_C
Cuts at: 0 501 2918 4281
Size: 501 2417 1363
Fragments arranged by size:
2417 1363 501
BanI G'GyrC_C
Cuts at: 0 415 598 757 881 1390 1631 2775 3235
Size: 415 183 159 124 509 241 1144 460
Cuts at: 3235 3315 3414 3466 4233 4281
Size: 80 99 52 767 48
Fragments arranged by size:
1144 767 509 460 415 241 183 159
124 99 80 52 48
BanII G_rGCy'C
Cuts at: 0 154 268 366 1681 2081 2367 2438 2573
Size: 154 114 98 1315 400 286 71 135
Cuts at: 2573 2673 2940 3032 3386 3545 3917 3937 4281
Size: 100 267 92 354 159 372 20 344
Fragments arranged by size:
1315 400 372 354 344 286 267 159
154 135 114 100 98 92 71 20
BbeI G_GCGC'C
Cuts at: 0 761 1394 3319 4281
Size: 761 633 1925 962
Fragments arranged by size:
1925 962 761 633
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 131 267 1324 2338 4281
Size: 103 28 136 1057 1014 1943
Fragments arranged by size:
1943 1057 1014 136 103 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1166 1200 4281
Size: 1166 34 3081
Fragments arranged by size:
3081 1166 34
BglI GCCn_nnn'nGGC
Cuts at: 0 3166 3528 4205 4281
Size: 3166 362 677 76
Fragments arranged by size:
3166 677 362 76
BlpI GC'TnA_GC
Cuts at: 0 454 811 820 4281
Size: 454 357 9 3461
Fragments arranged by size:
3461 454 357 9
Bme1580I G_kGCm'C
Cuts at: 0 268 380 420 683 884 2081 2367 2854
Size: 268 112 40 263 201 1197 286 487
Cuts at: 2854 2934 2940 3386 3419 3545 3917 4281
Size: 80 6 446 33 126 372 364
Fragments arranged by size:
1197 487 446 372 364 286 268 263
201 126 112 80 40 33 6
BmgBI CAC'GTC
Cuts at: 0 3141 4281
Size: 3141 1140
BmrI ACTGGGnnnn_n'
Cuts at: 0 1416 2244 2973 4143 4281
Size: 1416 828 729 1170 138
Fragments arranged by size:
1416 1170 828 729 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1909 2457 4281
Size: 1909 548 1824
Fragments arranged by size:
1909 1824 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 568 636 715 1267 1348 1882 1930
Size: 265 303 68 79 552 81 534 48
Cuts at: 1930 1939 2504 2533 2579 3046 3422 3496 3768
Size: 9 565 29 46 467 376 74 272
Cuts at: 3768 4281
Size: 513
Fragments arranged by size:
565 552 534 513 467 376 303 272
265 81 79 74 68 48 46 29
9
Bpu10I CC'TnA_GC
Cuts at: 0 1555 2039 2567 4190 4281
Size: 1555 484 528 1623 91
Fragments arranged by size:
1623 1555 528 484 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 2657 3610 3709 4116 4281
Size: 168 2489 953 99 407 165
Fragments arranged by size:
2489 953 407 168 165 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 2479 4281
Size: 2479 1802
BsaAI yAC'GTr
Cuts at: 0 741 1661 2685 4281
Size: 741 920 1024 1596
Fragments arranged by size:
1596 1024 920 741
BsaBI GATnn'nnATC
Cuts at: 0 1828 1930 4012 4281
Size: 1828 102 2082 269
Fragments arranged by size:
2082 1828 269 102
BsaHI Gr'CG_yC
Cuts at: 0 581 758 1391 3316 4281
Size: 581 177 633 1925 965
Fragments arranged by size:
1925 965 633 581 177
BsaWI w'CCGG_w
Cuts at: 0 4236 4281
Size: 4236 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1078 1108 1193 1196 1223 1226 1321 1351
Size: 1078 30 85 3 27 3 95 30
Cuts at: 1351 2953 2983 3827 3857 4281
Size: 1602 30 844 30 424
Fragments arranged by size:
1602 1078 844 424 95 85 30 30
30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1217 1368 1936 2002 2974 4281
Size: 1039 178 151 568 66 972 1307
Fragments arranged by size:
1307 1039 972 568 178 151 66
BseYI C'CCAG_C
Cuts at: 0 80 587 591 730 1166 1322 1613 1816
Size: 80 507 4 139 436 156 291 203
Cuts at: 1816 2111 2343 2366 2402 2437 2600 3374 3379
Size: 295 232 23 36 35 163 774 5
Cuts at: 3379 3406 3518 4042 4281
Size: 27 112 524 239
Fragments arranged by size:
774 524 507 436 295 291 239 232
203 163 156 139 112 80 36 35
27 23 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 1467 1683 2373 2663 3139 3198 3580
Size: 948 519 216 690 290 476 59 382
Cuts at: 3580 3659 3780 4281
Size: 79 121 501
Fragments arranged by size:
948 690 519 501 476 382 290 216
121 79 59
BsiHKAI G_wGCw'C
Cuts at: 0 162 314 380 701 983 1099 2216 2713
Size: 162 152 66 321 282 116 1117 497
Cuts at: 2713 3253 4130 4281
Size: 540 877 151
Fragments arranged by size:
1117 877 540 497 321 282 162 152
151 116 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2721 4281
Size: 2721 1560
Bsp1286I G_dGCh'C
Cuts at: 0 154 162 268 314 366 380 420 683
Size: 154 8 106 46 52 14 40 263
Cuts at: 683 701 884 983 1099 1681 2081 2216 2367
Size: 18 183 99 116 582 400 135 151
Cuts at: 2367 2438 2573 2673 2713 2854 2934 2940 3032
Size: 71 135 100 40 141 80 6 92
Cuts at: 3032 3253 3386 3419 3545 3917 3937 4130 4281
Size: 221 133 33 126 372 20 193 151
Fragments arranged by size:
582 400 372 263 221 193 183 154
151 151 141 135 135 133 126 116
106 100 99 92 80 71 52 46
40 40 33 20 18 14 8 6
BspHI T'CATG_A
Cuts at: 0 1484 4281
Size: 1484 2797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 561 1254 1608 2401 3301 4113 4281
Size: 561 693 354 793 900 812 168
Fragments arranged by size:
900 812 793 693 561 354 168
BsrBI CCG'CTC
Cuts at: 0 1115 1193 2292 4281
Size: 1115 78 1099 1989
Fragments arranged by size:
1989 1115 1099 78
BsrDI GCAATG_nn'
Cuts at: 0 662 1751 2335 3203 4281
Size: 662 1089 584 868 1078
Fragments arranged by size:
1089 1078 868 662 584
BsrFI r'CCGG_y
Cuts at: 0 566 772 800 1904 2822 3281 3354 3737
Size: 566 206 28 1104 918 459 73 383
Cuts at: 3737 3974 4281
Size: 237 307
Fragments arranged by size:
1104 918 566 459 383 307 237 206
73 28
BssHII G'CGCG_C
Cuts at: 0 707 835 4281
Size: 707 128 3446
Fragments arranged by size:
3446 707 128
BssSI C'ACGA_G
Cuts at: 0 3954 4281
Size: 3954 327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 722 3637 4009 4125 4281
Size: 722 2915 372 116 156
Fragments arranged by size:
2915 722 372 156 116
BstEII G'GTnAC_C
Cuts at: 0 562 1212 3672 4281
Size: 562 650 2460 609
Fragments arranged by size:
2460 650 609 562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 676 1642 2195 3044 3804 4035 4281
Size: 676 966 553 849 760 231 246
Fragments arranged by size:
966 849 760 676 553 246 231
BstYI r'GATC_y
Cuts at: 0 501 1925 2918 2963 4281
Size: 501 1424 993 45 1318
Fragments arranged by size:
1424 1318 993 501 45
Bsu36I CC'TnA_GG
Cuts at: 0 410 488 2770 4281
Size: 410 78 2282 1511
Fragments arranged by size:
2282 1511 410 78
BtgI C'CryG_G
Cuts at: 0 260 856 875 1669 2044 2647 2940 4113
Size: 260 596 19 794 375 603 293 1173
Cuts at: 4113 4281
Size: 168
Fragments arranged by size:
1173 794 603 596 375 293 260 168
19
BtsI GCAGTG_nn'
Cuts at: 0 934 1151 1234 3092 3647 3714 4281
Size: 934 217 83 1858 555 67 567
Fragments arranged by size:
1858 934 567 555 217 83 67
DraIII CAC_nnn'GTG
Cuts at: 0 384 2981 4281
Size: 384 2597 1300
Fragments arranged by size:
2597 1300 384
DrdI GACnn_nn'nnGTC
Cuts at: 0 655 4281
Size: 655 3626
EaeI y'GGCC_r
Cuts at: 0 44 257 507 1362 2197 2293 3074 3644
Size: 44 213 250 855 835 96 781 570
Cuts at: 3644 3829 4025 4281
Size: 185 196 256
Fragments arranged by size:
855 835 781 570 256 250 213 196
185 96 44
EarI CTCTTCn'nnn_
Cuts at: 0 1777 2783 3508 3985 4281
Size: 1777 1006 725 477 296
Fragments arranged by size:
1777 1006 725 477 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 265 568 636 715 1267 1348 1882
Size: 97 168 303 68 79 552 81 534
Cuts at: 1882 1930 1939 2350 2504 2533 2579 3046 3422
Size: 48 9 411 154 29 46 467 376
Cuts at: 3422 3496 3768 4204 4281
Size: 74 272 436 77
Fragments arranged by size:
552 534 467 436 411 376 303 272
168 154 97 81 79 77 74 68
48 46 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 972 1861 1897 2134 2699 3576 3664 4281
Size: 972 889 36 237 565 877 88 617
Fragments arranged by size:
972 889 877 617 565 237 88 36
EcoO109I rG'GnC_Cy
Cuts at: 0 85 265 348 849 999 1171 1514 1550
Size: 85 180 83 501 150 172 343 36
Cuts at: 1550 1939 2078 2363 2533 3383 3495 3542 3914
Size: 389 139 285 170 850 112 47 372
Cuts at: 3914 4248 4281
Size: 334 33
Fragments arranged by size:
850 501 389 372 343 334 285 180
172 170 150 139 112 85 83 47
36 33
FspI TGC'GCA
Cuts at: 0 896 4281
Size: 896 3385
HaeII r_GCGC'y
Cuts at: 0 306 761 920 1158 1394 1806 2267 2386
Size: 306 455 159 238 236 412 461 119
Cuts at: 2386 3172 3282 3319 3822 4043 4281
Size: 786 110 37 503 221 238
Fragments arranged by size:
786 503 461 455 412 306 238 238
236 221 159 119 110 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1196 1228 1321 1353 1694 1726 2574 2605
Size: 1196 32 93 32 341 32 848 31
Cuts at: 2605 2606 2637 2655 2687 4240 4272 4281
Size: 1 31 18 32 1553 32 9
Fragments arranged by size:
1553 1196 848 341 93 32 32 32
32 32 31 31 18 9 1
KasI G'GCGC_C
Cuts at: 0 757 1390 3315 4281
Size: 757 633 1925 966
Fragments arranged by size:
1925 966 757 633
KpnI G_GTAC'C
Cuts at: 0 4237 4281
Size: 4237 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2951 3752 4281
Size: 2951 801 529
Fragments arranged by size:
2951 801 529
MscI TGG'CCA
Cuts at: 0 509 2199 3646 4027 4281
Size: 509 1690 1447 381 254
Fragments arranged by size:
1690 1447 509 381 254
MslI CAynn'nnrTG
Cuts at: 0 1409 2193 2612 2685 3042 3241 3610 3805
Size: 1409 784 419 73 357 199 369 195
Cuts at: 3805 3988 4033 4281
Size: 183 45 248
Fragments arranged by size:
1409 784 419 369 357 248 199 195
183 73 45
MspA1I CmG'CkG
Cuts at: 0 591 719 734 807 858 877 1023 1037
Size: 591 128 15 73 51 19 146 14
Cuts at: 1037 1326 1371 1577 1690 2068 2126 2377 2455
Size: 289 45 206 113 378 58 251 78
Cuts at: 2455 3080 3224 3328 3433 4281
Size: 625 144 104 105 848
Fragments arranged by size:
848 625 591 378 289 251 206 146
144 128 113 105 104 78 73 58
51 45 19 15 14
NaeI GCC'GGC
Cuts at: 0 774 802 2824 3283 3739 3976 4281
Size: 774 28 2022 459 456 237 305
Fragments arranged by size:
2022 774 459 456 305 237 28
NarI GG'CG_CC
Cuts at: 0 758 1391 3316 4281
Size: 758 633 1925 965
Fragments arranged by size:
1925 965 758 633
NcoI C'CATG_G
Cuts at: 0 2647 2940 4113 4281
Size: 2647 293 1173 168
Fragments arranged by size:
2647 1173 293 168
NgoMIV G'CCGG_C
Cuts at: 0 772 800 2822 3281 3737 3974 4281
Size: 772 28 2022 459 456 237 307
Fragments arranged by size:
2022 772 459 456 307 237 28
NspI r_CATG'y
Cuts at: 0 1268 1458 3232 3615 3723 3903 4281
Size: 1268 190 1774 383 108 180 378
Fragments arranged by size:
1774 1268 383 378 190 180 108
PasI CC'CwG_GG
Cuts at: 0 357 2316 3622 3917 4281
Size: 357 1959 1306 295 364
Fragments arranged by size:
1959 1306 364 357 295
PciI A'CATG_T
Cuts at: 0 1454 3719 4281
Size: 1454 2265 562
Fragments arranged by size:
2265 1454 562
PflMI CCAn_nnn'nTGG
Cuts at: 0 3141 3838 4023 4281
Size: 3141 697 185 258
Fragments arranged by size:
3141 697 258 185
PfoI T'CCnGG_A
Cuts at: 0 1061 1859 1946 2783 3438 4079 4258 4281
Size: 1061 798 87 837 655 641 179 23
Fragments arranged by size:
1061 837 798 655 641 179 87 23
PmlI CAC'GTG
Cuts at: 0 741 1661 2685 4281
Size: 741 920 1024 1596
Fragments arranged by size:
1596 1024 920 741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4150 4182 4281
Size: 4150 32 99
Fragments arranged by size:
4150 99 32
PpuMI rG'GwC_Cy
Cuts at: 0 85 348 849 1171 1514 1550 1939 4248
Size: 85 263 501 322 343 36 389 2309
Cuts at: 4248 4281
Size: 33
Fragments arranged by size:
2309 501 389 343 322 263 85 36
33
PshAI GACnn'nnGTC
Cuts at: 0 1744 4281
Size: 1744 2537
PspOMI G'GGCC_C
Cuts at: 0 264 2077 2363 2936 3382 3541 3913 4281
Size: 264 1813 286 573 446 159 372 368
Fragments arranged by size:
1813 573 446 372 368 286 264 159
PstI C_TGCA'G
Cuts at: 0 228 796 1042 1582 2628 2887 3022 3709
Size: 228 568 246 540 1046 259 135 687
Cuts at: 3709 4281
Size: 572
Fragments arranged by size:
1046 687 572 568 540 259 246 228
135
PvuII CAG'CTG
Cuts at: 0 591 807 1037 1326 1577 1690 2126 2377
Size: 591 216 230 289 251 113 436 251
Cuts at: 2377 2455 3080 3328 4281
Size: 78 625 248 953
Fragments arranged by size:
953 625 591 436 289 251 251 248
230 216 113 78
SacII CC_GC'GG
Cuts at: 0 859 878 4281
Size: 859 19 3403
Fragments arranged by size:
3403 859 19
SanDI GG'GwC_CC
Cuts at: 0 85 348 849 1171 1514 1550 1939 4281
Size: 85 263 501 322 343 36 389 2342
Fragments arranged by size:
2342 501 389 343 322 263 85 36
SapI GCTCTTCn'nnn_
Cuts at: 0 2783 3508 3985 4281
Size: 2783 725 477 296
Fragments arranged by size:
2783 725 477 296
ScaI AGT'ACT
Cuts at: 0 4213 4281
Size: 4213 68
SexAI A'CCwGG_T
Cuts at: 0 380 518 4281
Size: 380 138 3763
Fragments arranged by size:
3763 380 138
SfcI C'TryA_G
Cuts at: 0 224 792 912 1038 1578 2121 2624 2883
Size: 224 568 120 126 540 543 503 259
Cuts at: 2883 3018 3705 3885 4281
Size: 135 687 180 396
Fragments arranged by size:
687 568 543 540 503 396 259 224
180 135 126 120
SfoI GGC'GCC
Cuts at: 0 759 1392 3317 4281
Size: 759 633 1925 964
Fragments arranged by size:
1925 964 759 633
SgrAI Cr'CCGG_yG
Cuts at: 0 3281 4281
Size: 3281 1000
SmaI CCC'GGG
Cuts at: 0 19 870 1540 2935 3493 3540 4281
Size: 19 851 670 1395 558 47 741
Fragments arranged by size:
1395 851 741 670 558 47 19
SmlI C'TyrA_G
Cuts at: 0 147 2672 3589 3688 4095 4281
Size: 147 2525 917 99 407 186
Fragments arranged by size:
2525 917 407 186 147 99
SrfI GCCC'GGGC
Cuts at: 0 2935 3540 4281
Size: 2935 605 741
Fragments arranged by size:
2935 741 605
StuI AGG'CCT
Cuts at: 0 1309 1767 2053 2206 2468 3016 4281
Size: 1309 458 286 153 262 548 1265
Fragments arranged by size:
1309 1265 548 458 286 262 153
StyI C'CwwG_G
Cuts at: 0 950 1100 1431 2172 2474 2647 2940 3040
Size: 950 150 331 741 302 173 293 100
Cuts at: 3040 3060 3087 3129 4113 4281
Size: 20 27 42 984 168
Fragments arranged by size:
984 950 741 331 302 293 173 168
150 100 42 27 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 455 601 1372 2046 2992 4063 4102 4281
Size: 455 146 771 674 946 1071 39 179
Fragments arranged by size:
1071 946 771 674 455 179 146 39
TatI w'GTAC_w
Cuts at: 0 1880 2867 4211 4281
Size: 1880 987 1344 70
Fragments arranged by size:
1880 1344 987 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1062 1078 1094 1110 1193 1225 1429 1461
Size: 1062 16 16 16 83 32 204 32
Cuts at: 1461 2779 2811 2965 2997 3827 3859 4281
Size: 1318 32 154 32 830 32 422
Fragments arranged by size:
1318 1062 830 422 204 154 83 32
32 32 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 0 520 4281
Size: 520 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 981 1642 2051 2148 3804 4020 4281
Size: 981 661 409 97 1656 216 261
Fragments arranged by size:
1656 981 661 409 261 216 97
XmaI C'CCGG_G
Cuts at: 0 17 868 1538 2933 3491 3538 4281
Size: 17 851 670 1395 558 47 743
Fragments arranged by size:
1395 851 743 670 558 47 17
XmnI GAAnn'nnTTC
Cuts at: 0 341 4281
Size: 341 3940
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SacII SanDI SapI ScaI SexAI SfcI SfoI
SgrAI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApoI AsiSI BaeI
BbvCI BciVI BclI BfrBI BglII BmtI BsiEI BsiWI
BsmI BspEI BsrGI BstBI BstZ17I BtgZI ClaI CspCI
DraI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PmeI PsiI PspXI PsrI
PvuI RsrII SacI SalI SbfI SfiI SgfI SnaBI
SpeI SphI SspI SwaI XbaI XhoI ZraI