Restriction map for
pFN21AE6739
(Linear) (Six-base) MAPSORT of: KIEE6739.seq Check: 2271 from: 1 to: 3654
KIEE6739.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 743 1171 2164 3654
Size: 743 428 993 1490
Fragments arranged by size:
1490 993 743 428
AccI GT'mk_AC
Cuts at: 0 3536 3654
Size: 3536 118
Acc65I G'GTAC_C
Cuts at: 0 2408 3654
Size: 2408 1246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1784 3002 3074 3205 3482 3654
Size: 529 1255 1218 72 131 277 172
Fragments arranged by size:
1255 1218 529 277 172 131 72
AflIII A'CryG_T
Cuts at: 0 2041 2272 3036 3654
Size: 2041 231 764 618
Fragments arranged by size:
2041 764 618 231
AgeI A'CCGG_T
Cuts at: 0 2411 2525 3654
Size: 2411 114 1129
Fragments arranged by size:
2411 1129 114
AleI CACnn'nnGTG
Cuts at: 0 2111 2275 2389 2552 2794 3654
Size: 2111 164 114 163 242 860
Fragments arranged by size:
2111 860 242 164 163 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 2570 3654
Size: 379 2191 1084
Fragments arranged by size:
2191 1084 379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3412 3444 3654
Size: 3412 32 210
Fragments arranged by size:
3412 210 32
AlwNI CAG_nnn'CTG
Cuts at: 0 507 949 1173 1533 2240 2655 3319 3654
Size: 507 442 224 360 707 415 664 335
Fragments arranged by size:
707 664 507 442 415 360 335 224
ApaI G_GGCC'C
Cuts at: 0 272 679 2330 2444 3654
Size: 272 407 1651 114 1210
Fragments arranged by size:
1651 1210 407 272 114
ApaLI G'TGCA_C
Cuts at: 0 195 2841 3654
Size: 195 2646 813
Fragments arranged by size:
2646 813 195
ApoI r'AATT_y
Cuts at: 0 1834 2952 3654
Size: 1834 1118 702
Fragments arranged by size:
1834 1118 702
AseI AT'TA_AT
Cuts at: 0 1352 1889 2003 3654
Size: 1352 537 114 1651
Fragments arranged by size:
1651 1352 537 114
AvaI C'yCGr_G
Cuts at: 0 93 180 471 804 1654 2427 3654
Size: 93 87 291 333 850 773 1227
Fragments arranged by size:
1227 850 773 333 291 93 87
BanI G'GyrC_C
Cuts at: 0 32 169 755 1159 1609 2152 2383 2408
Size: 32 137 586 404 450 543 231 25
Cuts at: 2408 2554 3272 3654
Size: 146 718 382
Fragments arranged by size:
718 586 543 450 404 382 231 146
137 32 25
BanII G_rGCy'C
Cuts at: 0 272 679 836 2330 2444 3223 3654
Size: 272 407 157 1494 114 779 431
Fragments arranged by size:
1494 779 431 407 272 157 114
BbeI G_GCGC'C
Cuts at: 0 36 173 3654
Size: 36 137 3481
Fragments arranged by size:
3481 137 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1306 2468 2804 2839 3654
Size: 1306 1162 336 35 815
Fragments arranged by size:
1306 1162 815 336 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 339 373 692 726 1453 1487 1914 1948
Size: 339 34 319 34 727 34 427 34
Cuts at: 1948 3654
Size: 1706
Fragments arranged by size:
1706 727 427 339 319 34 34 34
34
BclI T'GATC_A
Cuts at: 0 479 3654
Size: 479 3175
BglI GCCn_nnn'nGGC
Cuts at: 0 707 764 3654
Size: 707 57 2890
Fragments arranged by size:
2890 707 57
BglII A'GATC_T
Cuts at: 0 885 3654
Size: 885 2769
BlpI GC'TnA_GC
Cuts at: 0 1007 3217 3654
Size: 1007 2210 437
Fragments arranged by size:
2210 1007 437
Bme1580I G_kGCm'C
Cuts at: 0 69 199 272 679 1162 1201 1614 1654
Size: 69 130 73 407 483 39 413 40
Cuts at: 1654 2041 2272 2330 2427 2444 2549 2723 2734
Size: 387 231 58 97 17 105 174 11
Cuts at: 2734 2845 3624 3654
Size: 111 779 30
Fragments arranged by size:
779 483 413 407 387 231 174 130
111 105 97 73 69 58 40 39
30 17 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 870 1223 1608 3654
Size: 870 353 385 2046
Fragments arranged by size:
2046 870 385 353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3197 3654
Size: 3197 457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2525 2806 3654
Size: 2525 281 848
Fragments arranged by size:
2525 848 281
Bpu10I CC'TnA_GC
Cuts at: 0 40 285 719 3654
Size: 40 245 434 2935
Fragments arranged by size:
2935 434 245 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 310 3654
Size: 220 90 3344
Fragments arranged by size:
3344 220 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 179 847 1051 2574 3654
Size: 179 668 204 1523 1080
Fragments arranged by size:
1523 1080 668 204 179
BsaAI yAC'GTr
Cuts at: 0 2042 2113 2138 2300 3654
Size: 2042 71 25 162 1354
Fragments arranged by size:
2042 1354 162 71 25
BsaHI Gr'CG_yC
Cuts at: 0 33 170 1382 3654
Size: 33 137 1212 2272
Fragments arranged by size:
2272 1212 137 33
BsaWI w'CCGG_w
Cuts at: 0 2411 2525 3654
Size: 2411 114 1129
Fragments arranged by size:
2411 1129 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 232 262 2893 2923 3412 3442
Size: 103 30 99 30 2631 30 489 30
Cuts at: 3442 3654
Size: 212
Fragments arranged by size:
2631 489 212 103 99 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 487 1973 2201 2318 2437 2734 3654
Size: 487 1486 228 117 119 297 920
Fragments arranged by size:
1486 920 487 297 228 119 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1716 2772 3654
Size: 1716 1056 882
Fragments arranged by size:
1716 1056 882
BsiEI CG_ry'CG
Cuts at: 0 24 263 348 3654
Size: 24 239 85 3306
Fragments arranged by size:
3306 239 85 24
BsiHKAI G_wGCw'C
Cuts at: 0 199 1013 2340 2845 2933 3223 3654
Size: 199 814 1327 505 88 290 431
Fragments arranged by size:
1327 814 505 431 290 199 88
BsmI GAATG_Cn'
Cuts at: 0 2473 3654
Size: 2473 1181
Bsp1286I G_dGCh'C
Cuts at: 0 69 199 272 679 836 1013 1162 1201
Size: 69 130 73 407 157 177 149 39
Cuts at: 1201 1614 1654 2041 2272 2330 2340 2427 2444
Size: 413 40 387 231 58 10 87 17
Cuts at: 2444 2549 2723 2734 2845 2933 3223 3624 3654
Size: 105 174 11 111 88 290 401 30
Fragments arranged by size:
413 407 401 387 290 231 177 174
157 149 130 111 105 88 87 73
69 58 40 39 30 17 11 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 743 1171 2110 2164 2653 3654
Size: 743 428 939 54 489 1001
Fragments arranged by size:
1001 939 743 489 428 54
BsrDI GCAATG_nn'
Cuts at: 0 1289 3654
Size: 1289 2365
BsrFI r'CCGG_y
Cuts at: 0 23 491 1099 2411 2525 2826 3519 3654
Size: 23 468 608 1312 114 301 693 135
Fragments arranged by size:
1312 693 608 468 301 135 114 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1676 3654
Size: 1676 1978
BstYI r'GATC_y
Cuts at: 0 113 545 885 3242 3654
Size: 113 432 340 2357 412
Fragments arranged by size:
2357 432 412 340 113
Bsu36I CC'TnA_GG
Cuts at: 0 974 3654
Size: 974 2680
BtgI C'CryG_G
Cuts at: 0 10 336 816 1152 1742 2594 3654
Size: 10 326 480 336 590 852 1060
Fragments arranged by size:
1060 852 590 480 336 326 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2982 3654
Size: 2982 672
BtsI GCAGTG_nn'
Cuts at: 0 502 847 1695 2946 3654
Size: 502 345 848 1251 708
Fragments arranged by size:
1251 848 708 502 345
ClaI AT'CG_AT
Cuts at: 0 1463 1577 1934 3107 3654
Size: 1463 114 357 1173 547
Fragments arranged by size:
1463 1173 547 357 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 66 101 879 914 1776 1811 2130 2165
Size: 66 35 778 35 862 35 319 35
Cuts at: 2165 3654
Size: 1489
Fragments arranged by size:
1489 862 778 319 66 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 2485 3654
Size: 2485 1169
EaeI y'GGCC_r
Cuts at: 0 21 260 382 867 1365 1739 3041 3654
Size: 21 239 122 485 498 374 1302 613
Fragments arranged by size:
1302 613 498 485 374 239 122 21
EagI C'GGCC_G
Cuts at: 0 21 260 3654
Size: 21 239 3394
Fragments arranged by size:
3394 239 21
EarI CTCTTCn'nnn_
Cuts at: 0 3464 3539 3654
Size: 3464 75 115
Fragments arranged by size:
3464 115 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3303 3654
Size: 3303 351
EcoICRI GAG'CTC
Cuts at: 0 3221 3654
Size: 3221 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1784 2525 2806 3002 3074 3205 3482
Size: 529 1255 741 281 196 72 131 277
Cuts at: 3482 3654
Size: 172
Fragments arranged by size:
1255 741 529 281 277 196 172 131
72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 384 3654
Size: 384 3270
EcoO109I rG'GnC_Cy
Cuts at: 0 89 268 269 389 676 946 988 1170
Size: 89 179 1 120 287 270 42 182
Cuts at: 1170 1982 2326 3654
Size: 812 344 1328
Fragments arranged by size:
1328 812 344 287 270 182 179 120
89 42 1
HaeII r_GCGC'y
Cuts at: 0 36 173 3654
Size: 36 137 3481
Fragments arranged by size:
3481 137 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 232 264 2573 2605 2794 2826
Size: 103 32 97 32 2309 32 189 32
Cuts at: 2826 2893 2925 3654
Size: 67 32 729
Fragments arranged by size:
2309 729 189 103 97 67 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1179 1944 2283 3654
Size: 1179 765 339 1371
Fragments arranged by size:
1371 1179 765 339
HpaI GTT'AAC
Cuts at: 0 1179 3654
Size: 1179 2475
KasI G'GCGC_C
Cuts at: 0 32 169 3654
Size: 32 137 3485
Fragments arranged by size:
3485 137 32
KpnI G_GTAC'C
Cuts at: 0 2412 3654
Size: 2412 1242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1775 1811 3654
Size: 89 1686 36 1843
Fragments arranged by size:
1843 1686 89 36
MscI TGG'CCA
Cuts at: 0 1367 1741 3043 3654
Size: 1367 374 1302 611
Fragments arranged by size:
1367 1302 611 374
MslI CAynn'nnrTG
Cuts at: 0 2111 2275 2389 2552 2593 2794 3654
Size: 2111 164 114 163 41 201 860
Fragments arranged by size:
2111 860 201 164 163 114 41
MspA1I CmG'CkG
Cuts at: 0 338 1049 1569 1730 2381 2738 2980 3354
Size: 338 711 520 161 651 357 242 374
Cuts at: 3354 3654
Size: 300
Fragments arranged by size:
711 651 520 374 357 338 300 242
161
NaeI GCC'GGC
Cuts at: 0 493 3521 3654
Size: 493 3028 133
Fragments arranged by size:
3028 493 133
NarI GG'CG_CC
Cuts at: 0 33 170 3654
Size: 33 137 3484
Fragments arranged by size:
3484 137 33
NcoI C'CATG_G
Cuts at: 0 1742 2594 3654
Size: 1742 852 1060
Fragments arranged by size:
1742 1060 852
NgoMIV G'CCGG_C
Cuts at: 0 491 3519 3654
Size: 491 3028 135
Fragments arranged by size:
3028 491 135
NruI TCG'CGA
Cuts at: 0 2048 3654
Size: 2048 1606
NspI r_CATG'y
Cuts at: 0 1112 2190 2276 3040 3654
Size: 1112 1078 86 764 614
Fragments arranged by size:
1112 1078 764 614 86
PasI CC'CwG_GG
Cuts at: 0 1065 3654
Size: 1065 2589
PciI A'CATG_T
Cuts at: 0 2272 3036 3654
Size: 2272 764 618
Fragments arranged by size:
2272 764 618
PflMI CCAn_nnn'nTGG
Cuts at: 0 1173 3654
Size: 1173 2481
PfoI T'CCnGG_A
Cuts at: 0 2759 3654
Size: 2759 895
PmlI CAC'GTG
Cuts at: 0 2042 2113 2138 3654
Size: 2042 71 25 1516
Fragments arranged by size:
2042 1516 71 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3412 3444 3654
Size: 3412 32 210
Fragments arranged by size:
3412 210 32
PpuMI rG'GwC_Cy
Cuts at: 0 389 988 1170 3654
Size: 389 599 182 2484
Fragments arranged by size:
2484 599 389 182
PspOMI G'GGCC_C
Cuts at: 0 268 675 2326 2440 3654
Size: 268 407 1651 114 1214
Fragments arranged by size:
1651 1214 407 268 114
PstI C_TGCA'G
Cuts at: 0 149 277 715 754 2014 2359 2452 3654
Size: 149 128 438 39 1260 345 93 1202
Fragments arranged by size:
1260 1202 438 345 149 128 93 39
PvuII CAG'CTG
Cuts at: 0 1049 1569 1730 3354 3654
Size: 1049 520 161 1624 300
Fragments arranged by size:
1624 1049 520 300 161
RsrII CG'GwC_CG
Cuts at: 0 26 3654
Size: 26 3628
SacI G_AGCT'C
Cuts at: 0 3223 3654
Size: 3223 431
SacII CC_GC'GG
Cuts at: 0 339 3654
Size: 339 3315
SexAI A'CCwGG_T
Cuts at: 0 1172 2051 2913 3654
Size: 1172 879 862 741
Fragments arranged by size:
1172 879 862 741
SfcI C'TryA_G
Cuts at: 0 145 273 711 750 1962 2010 2355 2448
Size: 145 128 438 39 1212 48 345 93
Cuts at: 2448 3654
Size: 1206
Fragments arranged by size:
1212 1206 438 345 145 128 93 48
39
SfoI GGC'GCC
Cuts at: 0 34 171 3654
Size: 34 137 3483
Fragments arranged by size:
3483 137 34
SmaI CCC'GGG
Cuts at: 0 182 806 2429 3654
Size: 182 624 1623 1225
Fragments arranged by size:
1623 1225 624 182
SmlI C'TyrA_G
Cuts at: 0 235 325 3654
Size: 235 90 3329
Fragments arranged by size:
3329 235 90
SspI AAT'ATT
Cuts at: 0 1350 1887 2001 2961 2986 3654
Size: 1350 537 114 960 25 668
Fragments arranged by size:
1350 960 668 537 114 25
StuI AGG'CCT
Cuts at: 0 776 3654
Size: 776 2878
StyI C'CwwG_G
Cuts at: 0 77 1362 1742 2594 3654
Size: 77 1285 380 852 1060
Fragments arranged by size:
1285 1060 852 380 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2075 2798 3593 3654
Size: 2075 723 795 61
Fragments arranged by size:
2075 795 723 61
TatI w'GTAC_w
Cuts at: 0 779 3644 3654
Size: 779 2865 10
Fragments arranged by size:
2865 779 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2755 2787 3654
Size: 2755 32 867
Fragments arranged by size:
2755 867 32
Tth111I GACn'n_nGTC
Cuts at: 0 2053 3654
Size: 2053 1601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2656 2788 3654
Size: 2656 132 866
Fragments arranged by size:
2656 866 132
XmaI C'CCGG_G
Cuts at: 0 180 804 2427 3654
Size: 180 624 1623 1227
Fragments arranged by size:
1623 1227 624 180
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HpaI KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SexAI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AscI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BmgBI BmtI BsaBI
BseRI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI HindIII MfeI MluI
NdeI NheI NotI NsiI PacI PmeI PshAI PsiI
PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
XbaI XhoI XmnI ZraI