Restriction map for
pFN21AE6731
(Linear) (Six-base) MAPSORT of: KIEE6731.seq Check: 4391 from: 1 to: 4158
KIEE6731.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1134 2049 4158
Size: 1134 915 2109
Fragments arranged by size:
2109 1134 915
AccI GT'mk_AC
Cuts at: 0 419 864 4158
Size: 419 445 3294
Fragments arranged by size:
3294 445 419
Acc65I G'GTAC_C
Cuts at: 0 1510 4158
Size: 1510 2648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 770 1025 1168 1764 4158
Size: 281 489 255 143 596 2394
Fragments arranged by size:
2394 596 489 281 255 143
AflII C'TTAA_G
Cuts at: 0 1606 4158
Size: 1606 2552
AflIII A'CryG_T
Cuts at: 0 1265 4018 4158
Size: 1265 2753 140
Fragments arranged by size:
2753 1265 140
AhdI GACnn_n'nnGTC
Cuts at: 0 2163 4158
Size: 2163 1995
AleI CACnn'nnGTG
Cuts at: 0 427 976 1237 4158
Size: 427 549 261 2921
Fragments arranged by size:
2921 549 427 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 965 4158
Size: 965 3193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2366 2398 4158
Size: 2366 32 1760
Fragments arranged by size:
2366 1760 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1078 1161 1383 1470 1792 2001 2131 3692
Size: 1078 83 222 87 322 209 130 1561
Cuts at: 3692 4158
Size: 466
Fragments arranged by size:
1561 1078 466 322 222 209 130 87
83
ApaI G_GGCC'C
Cuts at: 0 3846 4081 4158
Size: 3846 235 77
Fragments arranged by size:
3846 235 77
ApaLI G'TGCA_C
Cuts at: 0 385 4158
Size: 385 3773
AvaI C'yCGr_G
Cuts at: 0 227 2614 2716 3998 4073 4158
Size: 227 2387 102 1282 75 85
Fragments arranged by size:
2387 1282 227 102 85 75
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2340 2373 4158
Size: 2340 33 1785
Fragments arranged by size:
2340 1785 33
BamHI G'GATC_C
Cuts at: 0 327 849 4158
Size: 327 522 3309
Fragments arranged by size:
3309 522 327
BanI G'GyrC_C
Cuts at: 0 361 392 1510 2426 2505 2741 3338 3392
Size: 361 31 1118 916 79 236 597 54
Cuts at: 3392 3537 4009 4158
Size: 145 472 149
Fragments arranged by size:
1118 916 597 472 361 236 149 145
79 54 31
BanII G_rGCy'C
Cuts at: 0 239 1674 1919 2033 2615 2672 2723 2765
Size: 239 1435 245 114 582 57 51 42
Cuts at: 2765 3377 3846 4054 4072 4081 4158
Size: 612 469 208 18 9 77
Fragments arranged by size:
1435 612 582 469 245 239 208 114
77 57 51 42 18 9
BbeI G_GCGC'C
Cuts at: 0 365 396 2745 4158
Size: 365 31 2349 1413
Fragments arranged by size:
2349 1413 365 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 1625 4158
Size: 1625 2533
BbvCI CC'TCA_GC
Cuts at: 0 3643 3853 4158
Size: 3643 210 305
Fragments arranged by size:
3643 305 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2643 2677 4158
Size: 2643 34 1481
Fragments arranged by size:
2643 1481 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3579 4158
Size: 3579 579
BglI GCCn_nnn'nGGC
Cuts at: 0 1194 2704 3546 4158
Size: 1194 1510 842 612
Fragments arranged by size:
1510 1194 842 612
BglII A'GATC_T
Cuts at: 0 213 2420 3126 4158
Size: 213 2207 706 1032
Fragments arranged by size:
2207 1032 706 213
BlpI GC'TnA_GC
Cuts at: 0 2764 4158
Size: 2764 1394
Bme1580I G_kGCm'C
Cuts at: 0 389 566 2481 2510 2567 2574 3028 3343
Size: 389 177 1915 29 57 7 454 315
Cuts at: 3343 3542 3681 3846 3926 4012 4081 4158
Size: 199 139 165 80 86 69 77
Fragments arranged by size:
1915 454 389 315 199 177 165 139
86 80 77 69 57 29 7
BmgBI CAC'GTC
Cuts at: 0 1032 1760 4158
Size: 1032 728 2398
Fragments arranged by size:
2398 1032 728
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 1166 1433 1632 1649 1754 2077 2297
Size: 1035 131 267 199 17 105 323 220
Cuts at: 2297 2561 2569 3644 4158
Size: 264 8 1075 514
Fragments arranged by size:
1075 1035 514 323 267 264 220 199
131 105 17 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 2844 3336 3396 3575 4158
Size: 789 2055 492 60 179 583
Fragments arranged by size:
2055 789 583 492 179 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 618 784 1100 1336 1345 1471 1852
Size: 60 558 166 316 236 9 126 381
Cuts at: 1852 2189 2629 2641 2852 3140 3159 3331 3366
Size: 337 440 12 211 288 19 172 35
Cuts at: 3366 3901 4038 4158
Size: 535 137 120
Fragments arranged by size:
558 535 440 381 337 316 288 236
211 172 166 137 126 120 60 35
19 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 3643 3853 4158
Size: 3643 210 305
Fragments arranged by size:
3643 305 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 664 4158
Size: 604 60 3494
Fragments arranged by size:
3494 604 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 228 1099 2518 4158
Size: 228 871 1419 1640
Fragments arranged by size:
1640 1419 871 228
BsaAI yAC'GTr
Cuts at: 0 1266 4158
Size: 1266 2892
BsaBI GATnn'nnATC
Cuts at: 0 643 4158
Size: 643 3515
BsaHI Gr'CG_yC
Cuts at: 0 362 393 2742 4158
Size: 362 31 2349 1416
Fragments arranged by size:
2349 1416 362 31
BsaWI w'CCGG_w
Cuts at: 0 174 914 3746 4158
Size: 174 740 2832 412
Fragments arranged by size:
2832 740 412 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1073 1103 2312 2342 2366 2396 3182 3212
Size: 1073 30 1209 30 24 30 786 30
Cuts at: 3212 3411 3441 4080 4086 4110 4116 4158
Size: 199 30 639 6 24 6 42
Fragments arranged by size:
1209 1073 786 639 199 42 30 30
30 30 30 24 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 1386 2308 2506 3094 3143 3254 3316
Size: 93 1293 922 198 588 49 111 62
Cuts at: 3316 3319 3346 4158
Size: 3 27 812
Fragments arranged by size:
1293 922 812 588 198 111 93 62
49 27 3
BseYI C'CCAG_C
Cuts at: 0 355 1052 1255 1785 1918 2253 2390 2474
Size: 355 697 203 530 133 335 137 84
Cuts at: 2474 3069 3376 3491 3572 3582 3839 3935 4158
Size: 595 307 115 81 10 257 96 223
Fragments arranged by size:
697 595 530 355 335 307 257 223
203 137 133 115 96 84 81 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1552 1846 2605 3241 4158
Size: 1261 291 294 759 636 917
Fragments arranged by size:
1261 917 759 636 294 291
BsiEI CG_ry'CG
Cuts at: 0 406 3032 4031 4158
Size: 406 2626 999 127
Fragments arranged by size:
2626 999 406 127
BsiHKAI G_wGCw'C
Cuts at: 0 389 2765 3741 3947 4158
Size: 389 2376 976 206 211
Fragments arranged by size:
2376 976 389 211 206
BsmI GAATG_Cn'
Cuts at: 0 562 2285 4158
Size: 562 1723 1873
Fragments arranged by size:
1873 1723 562
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1932 4158
Size: 1932 2226
Bsp1286I G_dGCh'C
Cuts at: 0 239 389 566 1674 1919 2033 2481 2510
Size: 239 150 177 1108 245 114 448 29
Cuts at: 2510 2567 2574 2615 2672 2723 2765 3028 3343
Size: 57 7 41 57 51 42 263 315
Cuts at: 3343 3377 3542 3681 3741 3846 3926 3947 4012
Size: 34 165 139 60 105 80 21 65
Cuts at: 4012 4054 4072 4081 4158
Size: 42 18 9 77
Fragments arranged by size:
1108 448 315 263 245 239 177 165
150 139 114 105 80 77 65 60
57 57 51 42 42 41 34 29
21 18 9 7
BspEI T'CCGG_A
Cuts at: 0 914 3746 4158
Size: 914 2832 412
Fragments arranged by size:
2832 914 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 893 1134 2049 4158
Size: 20 873 241 915 2109
Fragments arranged by size:
2109 915 873 241 20
BsrBI CCG'CTC
Cuts at: 0 1598 3885 4158
Size: 1598 2287 273
Fragments arranged by size:
2287 1598 273
BsrDI GCAATG_nn'
Cuts at: 0 2442 2495 4158
Size: 2442 53 1663
Fragments arranged by size:
2442 1663 53
BsrFI r'CCGG_y
Cuts at: 0 2796 4030 4158
Size: 2796 1234 128
Fragments arranged by size:
2796 1234 128
BsrGI T'GTAC_A
Cuts at: 0 1517 4158
Size: 1517 2641
BssHII G'CGCG_C
Cuts at: 0 398 4158
Size: 398 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 1470 4158
Size: 345 1125 2688
Fragments arranged by size:
2688 1125 345
BstEII G'GTnAC_C
Cuts at: 0 1047 3399 4158
Size: 1047 2352 759
Fragments arranged by size:
2352 1047 759
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2040 2398 3335 4158
Size: 2040 358 937 823
Fragments arranged by size:
2040 937 823 358
BstYI r'GATC_y
Cuts at: 0 213 327 849 911 2420 3126 4158
Size: 213 114 522 62 1509 706 1032
Fragments arranged by size:
1509 1032 706 522 213 114 62
Bsu36I CC'TnA_GG
Cuts at: 0 2132 2291 3120 4158
Size: 2132 159 829 1038
Fragments arranged by size:
2132 1038 829 159
BtgI C'CryG_G
Cuts at: 0 570 648 1235 1981 2852 3096 4025 4158
Size: 570 78 587 746 871 244 929 133
Fragments arranged by size:
929 871 746 587 570 244 133 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 701 1506 4158
Size: 701 805 2652
Fragments arranged by size:
2652 805 701
BtsI GCAGTG_nn'
Cuts at: 0 539 1471 1898 2807 3681 3900 4158
Size: 539 932 427 909 874 219 258
Fragments arranged by size:
932 909 874 539 427 258 219
ClaI AT'CG_AT
Cuts at: 0 2460 4158
Size: 2460 1698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 913 948 1025 1060 3794 3829 4158
Size: 913 35 77 35 2734 35 329
Fragments arranged by size:
2734 913 329 77 35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 415 4158
Size: 415 3743
EaeI y'GGCC_r
Cuts at: 0 56 311 403 573 1301 1502 1565 2540
Size: 56 255 92 170 728 201 63 975
Cuts at: 2540 3017 3029 3241 4028 4083 4158
Size: 477 12 212 787 55 75
Fragments arranged by size:
975 787 728 477 255 212 201 170
92 75 63 56 55 12
EagI C'GGCC_G
Cuts at: 0 403 3029 4028 4158
Size: 403 2626 999 130
Fragments arranged by size:
2626 999 403 130
EarI CTCTTCn'nnn_
Cuts at: 0 373 620 3770 3824 3953 4047 4158
Size: 373 247 3150 54 129 94 111
Fragments arranged by size:
3150 373 247 129 111 94 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 997 2020 4158
Size: 997 1023 2138
Fragments arranged by size:
2138 1023 997
EcoICRI GAG'CTC
Cuts at: 0 2763 4158
Size: 2763 1395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 281 618 770 784 1025 1100 1168
Size: 60 221 337 152 14 241 75 68
Cuts at: 1168 1336 1345 1471 1764 1852 2189 2629 2641
Size: 168 9 126 293 88 337 440 12
Cuts at: 2641 2852 3140 3159 3331 3366 3901 4038 4158
Size: 211 288 19 172 35 535 137 120
Fragments arranged by size:
535 440 337 337 293 288 241 221
211 172 168 152 137 126 120 88
75 68 60 35 19 14 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 1303 2135 3774 4158
Size: 835 468 832 1639 384
Fragments arranged by size:
1639 835 832 468 384
EcoO109I rG'GnC_Cy
Cuts at: 0 1158 1308 1905 1977 2044 2128 2210 2298
Size: 1158 150 597 72 67 84 82 88
Cuts at: 2298 2823 3046 3133 3730 3843 4077 4078 4158
Size: 525 223 87 597 113 234 1 80
Fragments arranged by size:
1158 597 597 525 234 223 150 113
88 87 84 82 80 72 67 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3220 4158
Size: 3220 938
FspI TGC'GCA
Cuts at: 0 338 784 4158
Size: 338 446 3374
Fragments arranged by size:
3374 446 338
FspAI rTGC'GCAy
Cuts at: 0 338 4158
Size: 338 3820
HaeII r_GCGC'y
Cuts at: 0 118 365 396 1200 2710 2745 4158
Size: 118 247 31 804 1510 35 1413
Fragments arranged by size:
1510 1413 804 247 118 35 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 2149 2181 3216 3248 4080 4086
Size: 1059 32 1058 32 1035 32 832 6
Cuts at: 4086 4112 4118 4158
Size: 26 6 40
Fragments arranged by size:
1059 1058 1035 832 40 32 32 32
26 6 6
HindIII A'AGCT_T
Cuts at: 0 3202 3987 4158
Size: 3202 785 171
Fragments arranged by size:
3202 785 171
KasI G'GCGC_C
Cuts at: 0 361 392 2741 4158
Size: 361 31 2349 1417
Fragments arranged by size:
2349 1417 361 31
KpnI G_GTAC'C
Cuts at: 0 1514 4158
Size: 1514 2644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 740 2135 4078 4158
Size: 95 645 1395 1943 80
Fragments arranged by size:
1943 1395 645 95 80
MscI TGG'CCA
Cuts at: 0 313 1303 1504 1567 3243 4158
Size: 313 990 201 63 1676 915
Fragments arranged by size:
1676 990 915 313 201 63
MslI CAynn'nnrTG
Cuts at: 0 427 700 947 976 1237 3953 4158
Size: 427 273 247 29 261 2716 205
Fragments arranged by size:
2716 427 273 261 247 205 29
MspA1I CmG'CkG
Cuts at: 0 359 572 748 1237 1559 1792 1830 1850
Size: 359 213 176 489 322 233 38 20
Cuts at: 1850 2091 2589 2690 3073 3939 4158
Size: 241 498 101 383 866 219
Fragments arranged by size:
866 498 489 383 359 322 241 233
219 213 176 101 38 20
NaeI GCC'GGC
Cuts at: 0 4032 4158
Size: 4032 126
NarI GG'CG_CC
Cuts at: 0 362 393 2742 4158
Size: 362 31 2349 1416
Fragments arranged by size:
2349 1416 362 31
NcoI C'CATG_G
Cuts at: 0 1981 2852 3096 4158
Size: 1981 871 244 1062
Fragments arranged by size:
1981 1062 871 244
NgoMIV G'CCGG_C
Cuts at: 0 4030 4158
Size: 4030 128
NspI r_CATG'y
Cuts at: 0 699 946 3510 3873 4022 4158
Size: 699 247 2564 363 149 136
Fragments arranged by size:
2564 699 363 247 149 136
PasI CC'CwG_GG
Cuts at: 0 2933 4158
Size: 2933 1225
PciI A'CATG_T
Cuts at: 0 4018 4158
Size: 4018 140
PflMI CCAn_nnn'nTGG
Cuts at: 0 1136 1744 1792 4158
Size: 1136 608 48 2366
Fragments arranged by size:
2366 1136 608 48
PfoI T'CCnGG_A
Cuts at: 0 513 595 1077 2065 4158
Size: 513 82 482 988 2093
Fragments arranged by size:
2093 988 513 482 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2366 2398 4158
Size: 2366 32 1760
Fragments arranged by size:
2366 1760 32
PpuMI rG'GwC_Cy
Cuts at: 0 1158 1977 2044 2210 3046 3133 3730 4158
Size: 1158 819 67 166 836 87 597 428
Fragments arranged by size:
1158 836 819 597 428 166 87 67
PshAI GACnn'nnGTC
Cuts at: 0 3450 4158
Size: 3450 708
PspOMI G'GGCC_C
Cuts at: 0 3842 4077 4158
Size: 3842 235 81
Fragments arranged by size:
3842 235 81
PstI C_TGCA'G
Cuts at: 0 32 1417 1475 1755 1859 2027 2060 2469
Size: 32 1385 58 280 104 168 33 409
Cuts at: 2469 3089 3630 4158
Size: 620 541 528
Fragments arranged by size:
1385 620 541 528 409 280 168 104
58 33 32
PvuII CAG'CTG
Cuts at: 0 748 1559 1792 1830 2091 2589 4158
Size: 748 811 233 38 261 498 1569
Fragments arranged by size:
1569 811 748 498 261 233 38
SacI G_AGCT'C
Cuts at: 0 2765 4158
Size: 2765 1393
SacII CC_GC'GG
Cuts at: 0 573 1238 4158
Size: 573 665 2920
Fragments arranged by size:
2920 665 573
SanDI GG'GwC_CC
Cuts at: 0 2044 2210 3133 4158
Size: 2044 166 923 1025
Fragments arranged by size:
2044 1025 923 166
SapI GCTCTTCn'nnn_
Cuts at: 0 3770 4158
Size: 3770 388
SbfI CC_TGCA'GG
Cuts at: 0 2027 2060 4158
Size: 2027 33 2098
Fragments arranged by size:
2098 2027 33
ScaI AGT'ACT
Cuts at: 0 551 2184 4158
Size: 551 1633 1974
Fragments arranged by size:
1974 1633 551
SfcI C'TryA_G
Cuts at: 0 28 1413 1471 1554 1646 1751 1855 2023
Size: 28 1385 58 83 92 105 104 168
Cuts at: 2023 2056 2161 2465 3054 3085 3626 3771 4158
Size: 33 105 304 589 31 541 145 387
Fragments arranged by size:
1385 589 541 387 304 168 145 105
105 104 92 83 58 33 31 28
SfoI GGC'GCC
Cuts at: 0 363 394 2743 4158
Size: 363 31 2349 1415
Fragments arranged by size:
2349 1415 363 31
SmaI CCC'GGG
Cuts at: 0 2616 4000 4075 4158
Size: 2616 1384 75 83
Fragments arranged by size:
2616 1384 83 75
SmlI C'TyrA_G
Cuts at: 0 619 679 1606 4158
Size: 619 60 927 2552
Fragments arranged by size:
2552 927 619 60
SrfI GCCC'GGGC
Cuts at: 0 2616 4158
Size: 2616 1542
StuI AGG'CCT
Cuts at: 0 1777 3603 3964 4158
Size: 1777 1826 361 194
Fragments arranged by size:
1826 1777 361 194
StyI C'CwwG_G
Cuts at: 0 1304 1981 2238 2333 2852 2859 3096 3185
Size: 1304 677 257 95 519 7 237 89
Cuts at: 3185 3543 3758 4158
Size: 358 215 400
Fragments arranged by size:
1304 677 519 400 358 257 237 215
95 89 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2865 2918 3604 3965 4158
Size: 2865 53 686 361 193
Fragments arranged by size:
2865 686 361 193 53
TatI w'GTAC_w
Cuts at: 0 549 1517 2182 4158
Size: 549 968 665 1976
Fragments arranged by size:
1976 968 665 549
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 594 626 1073 1105 1450 1482
Size: 509 32 53 32 447 32 345 32
Cuts at: 1482 2312 2344 3182 3214 4158
Size: 830 32 838 32 944
Fragments arranged by size:
944 838 830 509 447 345 53 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1311 1380 2553 2850 3175 3689 4158
Size: 1311 69 1173 297 325 514 469
Fragments arranged by size:
1311 1173 514 469 325 297 69
XmaI C'CCGG_G
Cuts at: 0 2614 3998 4073 4158
Size: 2614 1384 75 85
Fragments arranged by size:
2614 1384 85 75
XmnI GAAnn'nnTTC
Cuts at: 0 40 4158
Size: 40 4118
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SfcI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI ApoI AscI AseI AsiSI
AvrII BclI BfrBI BmtI BsiWI BspHI BssSI BstBI
BstZ17I DraI DraIII EcoRI EcoRV FseI HincII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI ZraI