Restriction map for
pFN21AE6727
(Linear) (Six-base) MAPSORT of: KIEE6727.seq Check: 9371 from: 1 to: 2136
KIEE6727.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 1061 2053 2136
Size: 1061 992 83
Fragments arranged by size:
1061 992 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 208 1057 1896 2136
Size: 29 179 849 839 240
Fragments arranged by size:
849 839 240 179 29
AflII C'TTAA_G
Cuts at: 0 1750 2136
Size: 1750 386
AhdI GACnn_n'nnGTC
Cuts at: 0 1366 2136
Size: 1366 770
AleI CACnn'nnGTG
Cuts at: 0 1567 2136
Size: 1567 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 707 2136
Size: 707 1429
ApoI r'AATT_y
Cuts at: 0 1246 1344 1596 2136
Size: 1246 98 252 540
Fragments arranged by size:
1246 540 252 98
AvrII C'CTAG_G
Cuts at: 0 1698 2136
Size: 1698 438
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1478 1511 2136
Size: 1478 33 625
Fragments arranged by size:
1478 625 33
BanII G_rGCy'C
Cuts at: 0 1190 1413 2136
Size: 1190 223 723
Fragments arranged by size:
1190 723 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 659 847 1902 2136
Size: 659 188 1055 234
Fragments arranged by size:
1055 659 234 188
BciVI GTATCCnnnnn_n'
Cuts at: 0 1708 2136
Size: 1708 428
BclI T'GATC_A
Cuts at: 0 960 2088 2136
Size: 960 1128 48
Fragments arranged by size:
1128 960 48
Bme1580I G_kGCm'C
Cuts at: 0 1951 2136
Size: 1951 185
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 1051 1342 1954 2136
Size: 798 253 291 612 182
Fragments arranged by size:
798 612 291 253 182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 440 2136
Size: 440 1696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 1796 2136
Size: 1149 647 340
Fragments arranged by size:
1149 647 340
Bpu10I CC'TnA_GC
Cuts at: 0 1966 2136
Size: 1966 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1416 2136
Size: 1416 720
BsaWI w'CCGG_w
Cuts at: 0 1591 2136
Size: 1591 545
BseYI C'CCAG_C
Cuts at: 0 1806 2136
Size: 1806 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1545 2136
Size: 703 842 591
Fragments arranged by size:
842 703 591
BsiHKAI G_wGCw'C
Cuts at: 0 196 1413 2136
Size: 196 1217 723
Fragments arranged by size:
1217 723 196
Bsp1286I G_dGCh'C
Cuts at: 0 196 1190 1413 1951 2136
Size: 196 994 223 538 185
Fragments arranged by size:
994 538 223 196 185
BspEI T'CCGG_A
Cuts at: 0 1591 2136
Size: 1591 545
BspHI T'CATG_A
Cuts at: 0 1401 2136
Size: 1401 735
BsrDI GCAATG_nn'
Cuts at: 0 386 623 1970 2136
Size: 386 237 1347 166
Fragments arranged by size:
1347 386 237 166
BsrGI T'GTAC_A
Cuts at: 0 1078 2136
Size: 1078 1058
BstEII G'GTnAC_C
Cuts at: 0 1303 2136
Size: 1303 833
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 2136
Size: 171 1965
BstYI r'GATC_y
Cuts at: 0 798 901 2136
Size: 798 103 1235
Fragments arranged by size:
1235 798 103
BstZ17I GTA'TAC
Cuts at: 0 1062 2136
Size: 1062 1074
Bsu36I CC'TnA_GG
Cuts at: 0 1602 2136
Size: 1602 534
BtsI GCAGTG_nn'
Cuts at: 0 367 1211 2136
Size: 367 844 925
Fragments arranged by size:
925 844 367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1515 1550 2136
Size: 1515 35 586
Fragments arranged by size:
1515 586 35
DraI TTT'AAA
Cuts at: 0 75 1863 1991 2136
Size: 75 1788 128 145
Fragments arranged by size:
1788 145 128 75
EaeI y'GGCC_r
Cuts at: 0 2113 2136
Size: 2113 23
EarI CTCTTCn'nnn_
Cuts at: 0 184 555 2136
Size: 184 371 1581
Fragments arranged by size:
1581 371 184
EcoICRI GAG'CTC
Cuts at: 0 1411 2136
Size: 1411 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 208 1057 1149 1796 1896 2136
Size: 29 179 849 92 647 100 240
Fragments arranged by size:
849 647 240 179 100 92 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1527 2136
Size: 1527 609
EcoRV GAT'ATC
Cuts at: 0 225 2136
Size: 225 1911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 86 150 2136
Size: 86 64 1986
Fragments arranged by size:
1986 86 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 532 564 1280 1312 1352 1384 1414
Size: 27 505 32 716 32 40 32 30
Cuts at: 1414 1446 2136
Size: 32 690
Fragments arranged by size:
716 690 505 40 32 32 32 32
30 27
HincII GTy'rAC
Cuts at: 0 753 2136
Size: 753 1383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 342 1550 2136
Size: 39 303 1208 586
Fragments arranged by size:
1208 586 303 39
MscI TGG'CCA
Cuts at: 0 2115 2136
Size: 2115 21
MslI CAynn'nnrTG
Cuts at: 0 1567 2136
Size: 1567 569
MspA1I CmG'CkG
Cuts at: 0 865 2136
Size: 865 1271
NdeI CA'TA_TG
Cuts at: 0 1491 2136
Size: 1491 645
PasI CC'CwG_GG
Cuts at: 0 984 2136
Size: 984 1152
PflMI CCAn_nnn'nTGG
Cuts at: 0 115 712 2111 2136
Size: 115 597 1399 25
Fragments arranged by size:
1399 597 115 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1397 1429 2136
Size: 1397 32 707
Fragments arranged by size:
1397 707 32
PsiI TTA'TAA
Cuts at: 0 286 2026 2136
Size: 286 1740 110
Fragments arranged by size:
1740 286 110
PvuII CAG'CTG
Cuts at: 0 865 2136
Size: 865 1271
SacI G_AGCT'C
Cuts at: 0 1413 2136
Size: 1413 723
SapI GCTCTTCn'nnn_
Cuts at: 0 184 555 2136
Size: 184 371 1581
Fragments arranged by size:
1581 371 184
ScaI AGT'ACT
Cuts at: 0 1979 2136
Size: 1979 157
SmlI C'TyrA_G
Cuts at: 0 1431 1750 2136
Size: 1431 319 386
Fragments arranged by size:
1431 386 319
SspI AAT'ATT
Cuts at: 0 1220 1545 2136
Size: 1220 325 591
Fragments arranged by size:
1220 591 325
StuI AGG'CCT
Cuts at: 0 66 2136
Size: 66 2070
StyI C'CwwG_G
Cuts at: 0 1212 1377 1698 1763 2136
Size: 1212 165 321 65 373
Fragments arranged by size:
1212 373 321 165 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 665 2136
Size: 665 1471
TatI w'GTAC_w
Cuts at: 0 404 1078 1133 1977 2136
Size: 404 674 55 844 159
Fragments arranged by size:
844 674 404 159 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1453 1485 1922 1954 2136
Size: 1453 32 437 32 182
Fragments arranged by size:
1453 437 182 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 2136
Size: 615 1521
XmnI GAAnn'nnTTC
Cuts at: 0 66 1246 2136
Size: 66 1180 890
Fragments arranged by size:
1180 890 66
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI ApoI AvrII
BaeI BanII BbsI BciVI BclI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaWI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BsrDI BsrGI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoRV FalI Hin4I HincII MmeI MscI MslI
MspA1I NdeI PasI PflMI PpiI PsiI PvuII SacI
SapI ScaI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BamHI
BanI BbeI BbvCI BcgI BfrBI BglI BglII BlpI
BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BtgI BtgZI ClaI DraIII DrdI
EagI EciI EcoO109I EcoRI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI
ZraI