Restriction map for
pFN21AE6723
(Linear) (Six-base) MAPSORT of: KIEE6723.seq Check: 5772 from: 1 to: 2097
KIEE6723.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
Acc65I G'GTAC_C
Cuts at: 0 949 2097
Size: 949 1148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 539 564 1129 1453 2097
Size: 119 420 25 565 324 644
Fragments arranged by size:
644 565 420 324 119 25
AflIII A'CryG_T
Cuts at: 0 1133 1529 1619 2097
Size: 1133 396 90 478
Fragments arranged by size:
1133 478 396 90
AhdI GACnn_n'nnGTC
Cuts at: 0 1635 2097
Size: 1635 462
AleI CACnn'nnGTG
Cuts at: 0 1900 2097
Size: 1900 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1111 2097
Size: 1111 986
AlwNI CAG_nnn'CTG
Cuts at: 0 97 1300 2097
Size: 97 1203 797
Fragments arranged by size:
1203 797 97
ApaI G_GGCC'C
Cuts at: 0 129 2097
Size: 129 1968
BanI G'GyrC_C
Cuts at: 0 949 1770 2097
Size: 949 821 327
Fragments arranged by size:
949 821 327
BanII G_rGCy'C
Cuts at: 0 129 449 2097
Size: 129 320 1648
Fragments arranged by size:
1648 320 129
BbeI G_GCGC'C
Cuts at: 0 1774 2097
Size: 1774 323
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 1656 2097
Size: 443 1213 441
Fragments arranged by size:
1213 443 441
BglII A'GATC_T
Cuts at: 0 686 1697 2097
Size: 686 1011 400
Fragments arranged by size:
1011 686 400
BlpI GC'TnA_GC
Cuts at: 0 1272 2097
Size: 1272 825
Bme1580I G_kGCm'C
Cuts at: 0 129 1546 1955 2097
Size: 129 1417 409 142
Fragments arranged by size:
1417 409 142 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 751 2097
Size: 751 1346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 609 2097
Size: 609 1488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1569 2097
Size: 184 1385 528
Fragments arranged by size:
1385 528 184
Bpu10I CC'TnA_GC
Cuts at: 0 539 2097
Size: 539 1558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 881 1222 1951 2097
Size: 733 148 341 729 146
Fragments arranged by size:
733 729 341 148 146
BsaAI yAC'GTr
Cuts at: 0 1176 1902 2097
Size: 1176 726 195
Fragments arranged by size:
1176 726 195
BsaBI GATnn'nnATC
Cuts at: 0 1729 2097
Size: 1729 368
BsaHI Gr'CG_yC
Cuts at: 0 1154 1610 1771 2097
Size: 1154 456 161 326
Fragments arranged by size:
1154 456 326 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 627 657 2097
Size: 627 30 1440
Fragments arranged by size:
1440 627 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 635 1564 2097
Size: 205 430 929 533
Fragments arranged by size:
929 533 430 205
BseYI C'CCAG_C
Cuts at: 0 496 1117 1253 2097
Size: 496 621 136 844
Fragments arranged by size:
844 621 496 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1462 1924 2041 2097
Size: 1462 462 117 56
Fragments arranged by size:
1462 462 117 56
BsiHKAI G_wGCw'C
Cuts at: 0 1573 2097
Size: 1573 524
BsmI GAATG_Cn'
Cuts at: 0 634 2097
Size: 634 1463
Bsp1286I G_dGCh'C
Cuts at: 0 129 449 1546 1573 1955 2097
Size: 129 320 1097 27 382 142
Fragments arranged by size:
1097 382 320 142 129 27
BspHI T'CATG_A
Cuts at: 0 1070 2097
Size: 1070 1027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 610 1299 2097
Size: 376 234 689 798
Fragments arranged by size:
798 689 376 234
BsrBI CCG'CTC
Cuts at: 0 861 963 2097
Size: 861 102 1134
Fragments arranged by size:
1134 861 102
BsrDI GCAATG_nn'
Cuts at: 0 890 2097
Size: 890 1207
BssSI C'ACGA_G
Cuts at: 0 1393 2097
Size: 1393 704
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2019 2097
Size: 2019 78
BstYI r'GATC_y
Cuts at: 0 686 1035 1697 2097
Size: 686 349 662 400
Fragments arranged by size:
686 662 400 349
Bsu36I CC'TnA_GG
Cuts at: 0 1463 2097
Size: 1463 634
BtgI C'CryG_G
Cuts at: 0 1451 1751 1947 2097
Size: 1451 300 196 150
Fragments arranged by size:
1451 300 196 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 386 1955 2097
Size: 386 1569 142
Fragments arranged by size:
1569 386 142
BtsI GCAGTG_nn'
Cuts at: 0 1004 1052 1496 2097
Size: 1004 48 444 601
Fragments arranged by size:
1004 601 444 48
DraIII CAC_nnn'GTG
Cuts at: 0 1485 2097
Size: 1485 612
EaeI y'GGCC_r
Cuts at: 0 782 1223 1877 2097
Size: 782 441 654 220
Fragments arranged by size:
782 654 441 220
EarI CTCTTCn'nnn_
Cuts at: 0 70 437 1393 1785 2097
Size: 70 367 956 392 312
Fragments arranged by size:
956 392 367 312 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 184 539 564 1129 1453 1569 2097
Size: 119 65 355 25 565 324 116 528
Fragments arranged by size:
565 528 355 324 119 116 65 25
EcoO109I rG'GnC_Cy
Cuts at: 0 590 2097
Size: 590 1507
FspI TGC'GCA
Cuts at: 0 1987 2097
Size: 1987 110
HaeII r_GCGC'y
Cuts at: 0 996 1774 2097
Size: 996 778 323
Fragments arranged by size:
996 778 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 807 839 1621 1653 1729 1761
Size: 627 32 148 32 782 32 76 32
Cuts at: 1761 2097
Size: 336
Fragments arranged by size:
782 627 336 148 76 32 32 32
32
HincII GTy'rAC
Cuts at: 0 170 870 2097
Size: 170 700 1227
Fragments arranged by size:
1227 700 170
KasI G'GCGC_C
Cuts at: 0 1770 2097
Size: 1770 327
KpnI G_GTAC'C
Cuts at: 0 953 2097
Size: 953 1144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 2097
Size: 1706 391
MscI TGG'CCA
Cuts at: 0 784 1225 2097
Size: 784 441 872
Fragments arranged by size:
872 784 441
MslI CAynn'nnrTG
Cuts at: 0 1174 1418 1624 1900 1933 2097
Size: 1174 244 206 276 33 164
Fragments arranged by size:
1174 276 244 206 164 33
MspA1I CmG'CkG
Cuts at: 0 162 496 505 1151 1476 2035 2097
Size: 162 334 9 646 325 559 62
Fragments arranged by size:
646 559 334 325 162 62 9
NarI GG'CG_CC
Cuts at: 0 1771 2097
Size: 1771 326
NdeI CA'TA_TG
Cuts at: 0 882 2097
Size: 882 1215
NspI r_CATG'y
Cuts at: 0 1092 1137 1533 1623 2097
Size: 1092 45 396 90 474
Fragments arranged by size:
1092 474 396 90 45
PciI A'CATG_T
Cuts at: 0 1133 1529 1619 2097
Size: 1133 396 90 478
Fragments arranged by size:
1133 478 396 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 680 1872 2097
Size: 680 1192 225
Fragments arranged by size:
1192 680 225
PfoI T'CCnGG_A
Cuts at: 0 617 1639 2097
Size: 617 1022 458
Fragments arranged by size:
1022 617 458
PmlI CAC'GTG
Cuts at: 0 1176 1902 2097
Size: 1176 726 195
Fragments arranged by size:
1176 726 195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 2097
Size: 1120 32 945
Fragments arranged by size:
1120 945 32
PspOMI G'GGCC_C
Cuts at: 0 125 2097
Size: 125 1972
PstI C_TGCA'G
Cuts at: 0 495 1270 1678 2097
Size: 495 775 408 419
Fragments arranged by size:
775 495 419 408
PvuII CAG'CTG
Cuts at: 0 162 496 505 1476 2035 2097
Size: 162 334 9 971 559 62
Fragments arranged by size:
971 559 334 162 62 9
SapI GCTCTTCn'nnn_
Cuts at: 0 437 2097
Size: 437 1660
ScaI AGT'ACT
Cuts at: 0 910 2097
Size: 910 1187
SexAI A'CCwGG_T
Cuts at: 0 941 1190 2069 2097
Size: 941 249 879 28
Fragments arranged by size:
941 879 249 28
SfcI C'TryA_G
Cuts at: 0 491 1101 1266 1674 2097
Size: 491 610 165 408 423
Fragments arranged by size:
610 491 423 408 165
SfoI GGC'GCC
Cuts at: 0 1772 2097
Size: 1772 325
SmlI C'TyrA_G
Cuts at: 0 712 896 1237 1966 2097
Size: 712 184 341 729 131
Fragments arranged by size:
729 712 341 184 131
SphI G_CATG'C
Cuts at: 0 1092 2097
Size: 1092 1005
StyI C'CwwG_G
Cuts at: 0 449 548 1658 2097
Size: 449 99 1110 439
Fragments arranged by size:
1110 449 439 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 328 1744 1912 1992 2097
Size: 187 141 1416 168 80 105
Fragments arranged by size:
1416 187 168 141 105 80
TatI w'GTAC_w
Cuts at: 0 908 1028 1130 2097
Size: 908 120 102 967
Fragments arranged by size:
967 908 120 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1443 1475 2097
Size: 1443 32 622
Fragments arranged by size:
1443 622 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1489 1932 2097
Size: 1489 443 165
Fragments arranged by size:
1489 443 165
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI
BanI BanII BbeI BbsI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EarI Eco57MI EcoO109I FspI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NdeI NspI PciI PflMI PfoI PmlI PpiI
PspOMI PstI PvuII SapI ScaI SexAI SfcI SfoI
SmlI SphI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AloI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI
BmtI BsaI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI MfeI MluI NaeI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI