Restriction map for
pFN21AE6722
(Linear) (Six-base) MAPSORT of: KIEE6722.seq Check: 3265 from: 1 to: 2295
KIEE6722.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 81 2295
Size: 81 2214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 1686 2295
Size: 549 1137 609
Fragments arranged by size:
1137 609 549
AflIII A'CryG_T
Cuts at: 0 643 1327 2295
Size: 643 684 968
Fragments arranged by size:
968 684 643
ApoI r'AATT_y
Cuts at: 0 16 2037 2081 2295
Size: 16 2021 44 214
Fragments arranged by size:
2021 214 44 16
BanII G_rGCy'C
Cuts at: 0 617 2295
Size: 617 1678
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 925 2295
Size: 555 370 1370
Fragments arranged by size:
1370 555 370
BciVI GTATCCnnnnn_n'
Cuts at: 0 1099 2005 2295
Size: 1099 906 290
Fragments arranged by size:
1099 906 290
BfrBI ATG'CAT
Cuts at: 0 1182 2295
Size: 1182 1113
BglII A'GATC_T
Cuts at: 0 723 1756 1830 2295
Size: 723 1033 74 465
Fragments arranged by size:
1033 723 465 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 1515 1833 2056 2295
Size: 1515 318 223 239
Fragments arranged by size:
1515 318 239 223
BmtI G_CTAG'C
Cuts at: 0 1588 2295
Size: 1588 707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 844 1093 1121 2076 2234 2295
Size: 724 120 249 28 955 158 61
Fragments arranged by size:
955 724 249 158 120 61 28
Bpu10I CC'TnA_GC
Cuts at: 0 1368 1627 2295
Size: 1368 259 668
Fragments arranged by size:
1368 668 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1215 1400 2295
Size: 620 595 185 895
Fragments arranged by size:
895 620 595 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 740 768 1072 1978 2295
Size: 135 605 28 304 906 317
Fragments arranged by size:
906 605 317 304 135 28
BsaAI yAC'GTr
Cuts at: 0 646 2295
Size: 646 1649
BseYI C'CCAG_C
Cuts at: 0 609 2295
Size: 609 1686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 2295
Size: 221 2074
BsiHKAI G_wGCw'C
Cuts at: 0 852 2220 2295
Size: 852 1368 75
Fragments arranged by size:
1368 852 75
BsmI GAATG_Cn'
Cuts at: 0 1458 1922 2295
Size: 1458 464 373
Fragments arranged by size:
1458 464 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1467 2295
Size: 1467 828
Bsp1286I G_dGCh'C
Cuts at: 0 617 852 2220 2295
Size: 617 235 1368 75
Fragments arranged by size:
1368 617 235 75
BspHI T'CATG_A
Cuts at: 0 525 857 2295
Size: 525 332 1438
Fragments arranged by size:
1438 525 332
BsrDI GCAATG_nn'
Cuts at: 0 1493 1535 2295
Size: 1493 42 760
Fragments arranged by size:
1493 760 42
BsrFI r'CCGG_y
Cuts at: 0 1899 2295
Size: 1899 396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 2295
Size: 606 1689
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1575 2295
Size: 1575 720
BstYI r'GATC_y
Cuts at: 0 723 1756 1830 2295
Size: 723 1033 74 465
Fragments arranged by size:
1033 723 465 74
Bsu36I CC'TnA_GG
Cuts at: 0 653 2295
Size: 653 1642
DraI TTT'AAA
Cuts at: 0 1194 2295
Size: 1194 1101
EaeI y'GGCC_r
Cuts at: 0 70 1023 2295
Size: 70 953 1272
Fragments arranged by size:
1272 953 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2016 2032 2295
Size: 2016 16 263
Fragments arranged by size:
2016 263 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 724 844 1093 1121 1686 2076 2234
Size: 549 175 120 249 28 565 390 158
Cuts at: 2234 2295
Size: 61
Fragments arranged by size:
565 549 390 249 175 158 120 61
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1418 2295
Size: 1418 877
EcoO109I rG'GnC_Cy
Cuts at: 0 889 1288 2295
Size: 889 399 1007
Fragments arranged by size:
1007 889 399
EcoRI G'AATT_C
Cuts at: 0 16 2295
Size: 16 2279
EcoRV GAT'ATC
Cuts at: 0 1038 2295
Size: 1038 1257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1775 1778 2295
Size: 1775 3 517
Fragments arranged by size:
1775 517 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 1437 1469 1827 1859 2295
Size: 32 32 1373 32 358 32 436
Fragments arranged by size:
1373 436 358 32 32 32 32
HincII GTy'rAC
Cuts at: 0 82 2166 2295
Size: 82 2084 129
Fragments arranged by size:
2084 129 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 471 701 885 2295
Size: 40 431 230 184 1410
Fragments arranged by size:
1410 431 230 184 40
MscI TGG'CCA
Cuts at: 0 72 1025 2295
Size: 72 953 1270
Fragments arranged by size:
1270 953 72
MslI CAynn'nnrTG
Cuts at: 0 454 1114 1332 1463 1483 1573 1685 2295
Size: 454 660 218 131 20 90 112 610
Fragments arranged by size:
660 610 454 218 131 112 90 20
MspA1I CmG'CkG
Cuts at: 0 1564 2295
Size: 1564 731
NheI G'CTAG_C
Cuts at: 0 1584 2295
Size: 1584 711
NsiI A_TGCA'T
Cuts at: 0 1184 2295
Size: 1184 1111
NspI r_CATG'y
Cuts at: 0 1182 1331 2295
Size: 1182 149 964
Fragments arranged by size:
1182 964 149
PciI A'CATG_T
Cuts at: 0 1327 2295
Size: 1327 968
PfoI T'CCnGG_A
Cuts at: 0 1070 2295
Size: 1070 1225
PmlI CAC'GTG
Cuts at: 0 646 2295
Size: 646 1649
PpuMI rG'GwC_Cy
Cuts at: 0 1288 2295
Size: 1288 1007
PstI C_TGCA'G
Cuts at: 0 602 2295
Size: 602 1693
PvuII CAG'CTG
Cuts at: 0 1564 2295
Size: 1564 731
SalI G'TCGA_C
Cuts at: 0 80 2295
Size: 80 2215
SfcI C'TryA_G
Cuts at: 0 479 598 812 2295
Size: 479 119 214 1483
Fragments arranged by size:
1483 479 214 119
SmlI C'TyrA_G
Cuts at: 0 635 1230 1415 2295
Size: 635 595 185 880
Fragments arranged by size:
880 635 595 185
StyI C'CwwG_G
Cuts at: 0 751 2295
Size: 751 1544
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 2295
Size: 1108 1187
TatI w'GTAC_w
Cuts at: 0 696 829 2295
Size: 696 133 1466
Fragments arranged by size:
1466 696 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1280 1285 1312 1329 1361 2295
Size: 1253 27 5 27 17 32 934
Fragments arranged by size:
1253 934 32 27 27 17 5
Tth111I GACn'n_nGTC
Cuts at: 0 1352 1658 1839 2295
Size: 1352 306 181 456
Fragments arranged by size:
1352 456 306 181
XmnI GAAnn'nnTTC
Cuts at: 0 20 173 1758 2084 2295
Size: 20 153 1585 326 211
Fragments arranged by size:
1585 326 211 153 20
Enzymes that do cut:
AccI AcuI AflIII ApoI BanII BbsI BciVI BfrBI
BglII BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrFI BstAPI BstXI BstYI Bsu36I DraI EaeI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
MmeI MscI MslI MspA1I NheI NsiI NspI PciI
PfoI PmlI PpuMI PstI PvuII SalI SfcI SmlI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BplI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI
EcoICRI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PasI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI ZraI