(Linear) (Six-base) MAPSORT of: KIEE6722.seq Check: 3265 from: 1 to: 2295 KIEE6722.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 81 2295 Size: 81 2214 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 1686 2295 Size: 549 1137 609 Fragments arranged by size: 1137 609 549 AflIII A'CryG_T Cuts at: 0 643 1327 2295 Size: 643 684 968 Fragments arranged by size: 968 684 643 ApoI r'AATT_y Cuts at: 0 16 2037 2081 2295 Size: 16 2021 44 214 Fragments arranged by size: 2021 214 44 16 BanII G_rGCy'C Cuts at: 0 617 2295 Size: 617 1678 BbsI GAAGACnn'nnnn_ Cuts at: 0 555 925 2295 Size: 555 370 1370 Fragments arranged by size: 1370 555 370 BciVI GTATCCnnnnn_n' Cuts at: 0 1099 2005 2295 Size: 1099 906 290 Fragments arranged by size: 1099 906 290 BfrBI ATG'CAT Cuts at: 0 1182 2295 Size: 1182 1113 BglII A'GATC_T Cuts at: 0 723 1756 1830 2295 Size: 723 1033 74 465 Fragments arranged by size: 1033 723 465 74 BmrI ACTGGGnnnn_n' Cuts at: 0 1515 1833 2056 2295 Size: 1515 318 223 239 Fragments arranged by size: 1515 318 239 223 BmtI G_CTAG'C Cuts at: 0 1588 2295 Size: 1588 707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 844 1093 1121 2076 2234 2295 Size: 724 120 249 28 955 158 61 Fragments arranged by size: 955 724 249 158 120 61 28 Bpu10I CC'TnA_GC Cuts at: 0 1368 1627 2295 Size: 1368 259 668 Fragments arranged by size: 1368 668 259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 620 1215 1400 2295 Size: 620 595 185 895 Fragments arranged by size: 895 620 595 185 BsaI GGTCTCn'nnnn_ Cuts at: 0 135 740 768 1072 1978 2295 Size: 135 605 28 304 906 317 Fragments arranged by size: 906 605 317 304 135 28 BsaAI yAC'GTr Cuts at: 0 646 2295 Size: 646 1649 BseYI C'CCAG_C Cuts at: 0 609 2295 Size: 609 1686 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 221 2295 Size: 221 2074 BsiHKAI G_wGCw'C Cuts at: 0 852 2220 2295 Size: 852 1368 75 Fragments arranged by size: 1368 852 75 BsmI GAATG_Cn' Cuts at: 0 1458 1922 2295 Size: 1458 464 373 Fragments arranged by size: 1458 464 373 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1467 2295 Size: 1467 828 Bsp1286I G_dGCh'C Cuts at: 0 617 852 2220 2295 Size: 617 235 1368 75 Fragments arranged by size: 1368 617 235 75 BspHI T'CATG_A Cuts at: 0 525 857 2295 Size: 525 332 1438 Fragments arranged by size: 1438 525 332 BsrDI GCAATG_nn' Cuts at: 0 1493 1535 2295 Size: 1493 42 760 Fragments arranged by size: 1493 760 42 BsrFI r'CCGG_y Cuts at: 0 1899 2295 Size: 1899 396 BstAPI GCAn_nnn'nTGC Cuts at: 0 606 2295 Size: 606 1689 BstXI CCAn_nnnn'nTGG Cuts at: 0 1575 2295 Size: 1575 720 BstYI r'GATC_y Cuts at: 0 723 1756 1830 2295 Size: 723 1033 74 465 Fragments arranged by size: 1033 723 465 74 Bsu36I CC'TnA_GG Cuts at: 0 653 2295 Size: 653 1642 DraI TTT'AAA Cuts at: 0 1194 2295 Size: 1194 1101 EaeI y'GGCC_r Cuts at: 0 70 1023 2295 Size: 70 953 1272 Fragments arranged by size: 1272 953 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2016 2032 2295 Size: 2016 16 263 Fragments arranged by size: 2016 263 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 724 844 1093 1121 1686 2076 2234 Size: 549 175 120 249 28 565 390 158 Cuts at: 2234 2295 Size: 61 Fragments arranged by size: 565 549 390 249 175 158 120 61 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 1418 2295 Size: 1418 877 EcoO109I rG'GnC_Cy Cuts at: 0 889 1288 2295 Size: 889 399 1007 Fragments arranged by size: 1007 889 399 EcoRI G'AATT_C Cuts at: 0 16 2295 Size: 16 2279 EcoRV GAT'ATC Cuts at: 0 1038 2295 Size: 1038 1257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1775 1778 2295 Size: 1775 3 517 Fragments arranged by size: 1775 517 3 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 32 64 1437 1469 1827 1859 2295 Size: 32 32 1373 32 358 32 436 Fragments arranged by size: 1373 436 358 32 32 32 32 HincII GTy'rAC Cuts at: 0 82 2166 2295 Size: 82 2084 129 Fragments arranged by size: 2084 129 82 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 40 471 701 885 2295 Size: 40 431 230 184 1410 Fragments arranged by size: 1410 431 230 184 40 MscI TGG'CCA Cuts at: 0 72 1025 2295 Size: 72 953 1270 Fragments arranged by size: 1270 953 72 MslI CAynn'nnrTG Cuts at: 0 454 1114 1332 1463 1483 1573 1685 2295 Size: 454 660 218 131 20 90 112 610 Fragments arranged by size: 660 610 454 218 131 112 90 20 MspA1I CmG'CkG Cuts at: 0 1564 2295 Size: 1564 731 NheI G'CTAG_C Cuts at: 0 1584 2295 Size: 1584 711 NsiI A_TGCA'T Cuts at: 0 1184 2295 Size: 1184 1111 NspI r_CATG'y Cuts at: 0 1182 1331 2295 Size: 1182 149 964 Fragments arranged by size: 1182 964 149 PciI A'CATG_T Cuts at: 0 1327 2295 Size: 1327 968 PfoI T'CCnGG_A Cuts at: 0 1070 2295 Size: 1070 1225 PmlI CAC'GTG Cuts at: 0 646 2295 Size: 646 1649 PpuMI rG'GwC_Cy Cuts at: 0 1288 2295 Size: 1288 1007 PstI C_TGCA'G Cuts at: 0 602 2295 Size: 602 1693 PvuII CAG'CTG Cuts at: 0 1564 2295 Size: 1564 731 SalI G'TCGA_C Cuts at: 0 80 2295 Size: 80 2215 SfcI C'TryA_G Cuts at: 0 479 598 812 2295 Size: 479 119 214 1483 Fragments arranged by size: 1483 479 214 119 SmlI C'TyrA_G Cuts at: 0 635 1230 1415 2295 Size: 635 595 185 880 Fragments arranged by size: 880 635 595 185 StyI C'CwwG_G Cuts at: 0 751 2295 Size: 751 1544 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1108 2295 Size: 1108 1187 TatI w'GTAC_w Cuts at: 0 696 829 2295 Size: 696 133 1466 Fragments arranged by size: 1466 696 133 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1253 1280 1285 1312 1329 1361 2295 Size: 1253 27 5 27 17 32 934 Fragments arranged by size: 1253 934 32 27 27 17 5 Tth111I GACn'n_nGTC Cuts at: 0 1352 1658 1839 2295 Size: 1352 306 181 456 Fragments arranged by size: 1352 456 306 181 XmnI GAAnn'nnTTC Cuts at: 0 20 173 1758 2084 2295 Size: 20 153 1585 326 211 Fragments arranged by size: 1585 326 211 153 20 Enzymes that do cut: AccI AcuI AflIII ApoI BanII BbsI BciVI BfrBI BglII BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I DraI EaeI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII MmeI MscI MslI MspA1I NheI NsiI NspI PciI PfoI PmlI PpuMI PstI PvuII SalI SfcI SmlI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BplI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI EcoICRI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PasI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI ZraI