Restriction map for
pFN21AE6718
(Linear) (Six-base) MAPSORT of: KIEE6718.seq Check: 3955 from: 1 to: 2046
KIEE6718.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 1663 2046
Size: 103 1560 383
Fragments arranged by size:
1560 383 103
AclI AA'CG_TT
Cuts at: 0 505 2046
Size: 505 1541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 1502 1747 2046
Size: 948 554 245 299
Fragments arranged by size:
948 554 299 245
AfeI AGC'GCT
Cuts at: 0 322 795 889 2046
Size: 322 473 94 1157
Fragments arranged by size:
1157 473 322 94
AleI CACnn'nnGTG
Cuts at: 0 1231 2046
Size: 1231 815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 2046
Size: 625 1421
AlwNI CAG_nnn'CTG
Cuts at: 0 87 856 1968 2046
Size: 87 769 1112 78
Fragments arranged by size:
1112 769 87 78
ApaI G_GGCC'C
Cuts at: 0 1739 2046
Size: 1739 307
ApaLI G'TGCA_C
Cuts at: 0 675 2046
Size: 675 1371
ApoI r'AATT_y
Cuts at: 0 340 391 769 1035 1490 2046
Size: 340 51 378 266 455 556
Fragments arranged by size:
556 455 378 340 266 51
AvaI C'yCGr_G
Cuts at: 0 911 2046
Size: 911 1135
BamHI G'GATC_C
Cuts at: 0 1788 2046
Size: 1788 258
BanII G_rGCy'C
Cuts at: 0 1604 1671 1739 2046
Size: 1604 67 68 307
Fragments arranged by size:
1604 307 68 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 1490 2046
Size: 1490 556
BbvCI CC'TCA_GC
Cuts at: 0 317 2046
Size: 317 1729
BclI T'GATC_A
Cuts at: 0 1550 2046
Size: 1550 496
BglI GCCn_nnn'nGGC
Cuts at: 0 853 1271 1937 2046
Size: 853 418 666 109
Fragments arranged by size:
853 666 418 109
BglII A'GATC_T
Cuts at: 0 53 381 986 1534 2046
Size: 53 328 605 548 512
Fragments arranged by size:
605 548 512 328 53
Bme1580I G_kGCm'C
Cuts at: 0 679 1739 2046
Size: 679 1060 307
Fragments arranged by size:
1060 679 307
BmgBI CAC'GTC
Cuts at: 0 674 2046
Size: 674 1372
BmrI ACTGGGnnnn_n'
Cuts at: 0 290 2046
Size: 290 1756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 703 850 1591 1618 1918 2046
Size: 109 594 147 741 27 300 128
Fragments arranged by size:
741 594 300 147 128 109 27
Bpu10I CC'TnA_GC
Cuts at: 0 317 1544 1945 2046
Size: 317 1227 401 101
Fragments arranged by size:
1227 401 317 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1551 2046
Size: 1551 495
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 1270 1366 2046
Size: 433 837 96 680
Fragments arranged by size:
837 680 433 96
BsaBI GATnn'nnATC
Cuts at: 0 658 2046
Size: 658 1388
BsaHI Gr'CG_yC
Cuts at: 0 1337 1385 2046
Size: 1337 48 661
Fragments arranged by size:
1337 661 48
BsaWI w'CCGG_w
Cuts at: 0 623 2046
Size: 623 1423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 569 599 625 655 2046
Size: 569 30 26 30 1391
Fragments arranged by size:
1391 569 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1588 2046
Size: 1588 458
BseYI C'CCAG_C
Cuts at: 0 275 1717 2046
Size: 275 1442 329
Fragments arranged by size:
1442 329 275
BsiEI CG_ry'CG
Cuts at: 0 125 2046
Size: 125 1921
BsiHKAI G_wGCw'C
Cuts at: 0 679 809 1058 1604 1864 2046
Size: 679 130 249 546 260 182
Fragments arranged by size:
679 546 260 249 182 130
BsmI GAATG_Cn'
Cuts at: 0 541 2046
Size: 541 1505
Bsp1286I G_dGCh'C
Cuts at: 0 679 809 1058 1604 1671 1739 1864 2046
Size: 679 130 249 546 67 68 125 182
Fragments arranged by size:
679 546 249 182 130 125 68 67
BspEI T'CCGG_A
Cuts at: 0 623 2046
Size: 623 1423
BspHI T'CATG_A
Cuts at: 0 950 2046
Size: 950 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 1663 2046
Size: 103 1560 383
Fragments arranged by size:
1560 383 103
BsrDI GCAATG_nn'
Cuts at: 0 260 2046
Size: 260 1786
BstBI TT'CG_AA
Cuts at: 0 59 992 2046
Size: 59 933 1054
Fragments arranged by size:
1054 933 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1350 1659 2022 2046
Size: 1350 309 363 24
Fragments arranged by size:
1350 363 309 24
BstYI r'GATC_y
Cuts at: 0 53 381 653 986 1534 1580 1788 2046
Size: 53 328 272 333 548 46 208 258
Fragments arranged by size:
548 333 328 272 258 208 53 46
BtgI C'CryG_G
Cuts at: 0 285 764 1145 1280 1317 1331 1349 1524
Size: 285 479 381 135 37 14 18 175
Cuts at: 1524 2046
Size: 522
Fragments arranged by size:
522 479 381 285 175 135 37 18
14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 429 828 2046
Size: 429 399 1218
Fragments arranged by size:
1218 429 399
BtsI GCAGTG_nn'
Cuts at: 0 857 2046
Size: 857 1189
ClaI AT'CG_AT
Cuts at: 0 125 2046
Size: 125 1921
DraIII CAC_nnn'GTG
Cuts at: 0 1923 2046
Size: 1923 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 1384 2046
Size: 1384 662
EaeI y'GGCC_r
Cuts at: 0 893 1418 2024 2046
Size: 893 525 606 22
Fragments arranged by size:
893 606 525 22
EarI CTCTTCn'nnn_
Cuts at: 0 712 2046
Size: 712 1334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 723 2046
Size: 723 1323
EcoICRI GAG'CTC
Cuts at: 0 1602 2046
Size: 1602 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 703 850 948 1502 1591 1618 1747
Size: 109 594 147 98 554 89 27 129
Cuts at: 1747 1918 2046
Size: 171 128
Fragments arranged by size:
594 554 171 147 129 128 109 98
89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 2046
Size: 70 1976
EcoO109I rG'GnC_Cy
Cuts at: 0 75 291 1736 2046
Size: 75 216 1445 310
Fragments arranged by size:
1445 310 216 75
EcoRI G'AATT_C
Cuts at: 0 769 2046
Size: 769 1277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1005 1095 2046
Size: 72 933 90 951
Fragments arranged by size:
951 933 90 72
HaeII r_GCGC'y
Cuts at: 0 324 797 891 1300 1325 2046
Size: 324 473 94 409 25 721
Fragments arranged by size:
721 473 409 324 94 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 663 695 892 924 1094 1126
Size: 406 32 225 32 197 32 170 32
Cuts at: 1126 2046
Size: 920
Fragments arranged by size:
920 406 225 197 170 32 32 32
32
HincII GTy'rAC
Cuts at: 0 306 2007 2046
Size: 306 1701 39
Fragments arranged by size:
1701 306 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 2046
Size: 512 1534
MscI TGG'CCA
Cuts at: 0 1420 2026 2046
Size: 1420 606 20
Fragments arranged by size:
1420 606 20
MslI CAynn'nnrTG
Cuts at: 0 1231 2046
Size: 1231 815
MspA1I CmG'CkG
Cuts at: 0 84 165 1282 1319 1959 1965 2046
Size: 84 81 1117 37 640 6 81
Fragments arranged by size:
1117 640 84 81 81 37 6
NruI TCG'CGA
Cuts at: 0 1370 2046
Size: 1370 676
NspI r_CATG'y
Cuts at: 0 748 1698 2046
Size: 748 950 348
Fragments arranged by size:
950 748 348
PflMI CCAn_nnn'nTGG
Cuts at: 0 1590 1923 2046
Size: 1590 333 123
Fragments arranged by size:
1590 333 123
PfoI T'CCnGG_A
Cuts at: 0 827 2046
Size: 827 1219
PpuMI rG'GwC_Cy
Cuts at: 0 291 2046
Size: 291 1755
PspOMI G'GGCC_C
Cuts at: 0 1735 2046
Size: 1735 311
PstI C_TGCA'G
Cuts at: 0 83 944 1823 1964 2046
Size: 83 861 879 141 82
Fragments arranged by size:
879 861 141 83 82
PvuI CG_AT'CG
Cuts at: 0 125 2046
Size: 125 1921
PvuII CAG'CTG
Cuts at: 0 165 1965 2046
Size: 165 1800 81
Fragments arranged by size:
1800 165 81
SacI G_AGCT'C
Cuts at: 0 1604 2046
Size: 1604 442
SacII CC_GC'GG
Cuts at: 0 1283 1320 2046
Size: 1283 37 726
Fragments arranged by size:
1283 726 37
SanDI GG'GwC_CC
Cuts at: 0 291 2046
Size: 291 1755
SfcI C'TryA_G
Cuts at: 0 27 79 940 1819 1960 2046
Size: 27 52 861 879 141 86
Fragments arranged by size:
879 861 141 86 52 27
SmlI C'TyrA_G
Cuts at: 0 911 1530 2046
Size: 911 619 516
Fragments arranged by size:
911 619 516
SphI G_CATG'C
Cuts at: 0 748 2046
Size: 748 1298
StyI C'CwwG_G
Cuts at: 0 1427 1865 2046
Size: 1427 438 181
Fragments arranged by size:
1427 438 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 1051 1390 1502 2046
Size: 298 753 339 112 544
Fragments arranged by size:
753 544 339 298 112
TatI w'GTAC_w
Cuts at: 0 92 2046
Size: 92 1954
XhoI C'TCGA_G
Cuts at: 0 911 2046
Size: 911 1135
Enzymes that do cut:
AarI AclI AcuI AfeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BclI
BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NruI NspI PflMI PfoI PpuMI PspOMI PstI PvuI
PvuII SacI SacII SanDI SfcI SmlI SphI StyI
TaqII TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI
BciVI BfrBI BlpI BmtI BplI BsaAI BsgI BsiWI
BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I CspCI DraI EagI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI RsrII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI