Restriction map for
pFN21AE6715
(Linear) (Six-base) MAPSORT of: KIEE6715.seq Check: 8833 from: 1 to: 2244
KIEE6715.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1500 2244
Size: 1500 744
AatII G_ACGT'C
Cuts at: 0 487 2244
Size: 487 1757
AccI GT'mk_AC
Cuts at: 0 155 2244
Size: 155 2089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1977 2244
Size: 688 1289 267
Fragments arranged by size:
1289 688 267
AfeI AGC'GCT
Cuts at: 0 913 2244
Size: 913 1331
AflIII A'CryG_T
Cuts at: 0 176 2244
Size: 176 2068
AhdI GACnn_n'nnGTC
Cuts at: 0 36 2244
Size: 36 2208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 544 2244
Size: 358 186 1700
Fragments arranged by size:
1700 358 186
AlwNI CAG_nnn'CTG
Cuts at: 0 1589 1962 2031 2244
Size: 1589 373 69 213
Fragments arranged by size:
1589 373 213 69
AvaI C'yCGr_G
Cuts at: 0 1172 1372 1503 1673 1696 1933 2244
Size: 1172 200 131 170 23 237 311
Fragments arranged by size:
1172 311 237 200 170 131 23
BanI G'GyrC_C
Cuts at: 0 187 956 991 1390 1633 1900 2244
Size: 187 769 35 399 243 267 344
Fragments arranged by size:
769 399 344 267 243 187 35
BanII G_rGCy'C
Cuts at: 0 727 926 1372 1615 2018 2063 2153 2170
Size: 727 199 446 243 403 45 90 17
Cuts at: 2170 2244
Size: 74
Fragments arranged by size:
727 446 403 243 199 90 74 45
17
BbeI G_GCGC'C
Cuts at: 0 960 1904 2244
Size: 960 944 340
Fragments arranged by size:
960 944 340
BbvCI CC'TCA_GC
Cuts at: 0 806 1855 2244
Size: 806 1049 389
Fragments arranged by size:
1049 806 389
BglI GCCn_nnn'nGGC
Cuts at: 0 101 547 817 1102 1787 2244
Size: 101 446 270 285 685 457
Fragments arranged by size:
685 457 446 285 270 101
BlpI GC'TnA_GC
Cuts at: 0 1109 1822 2169 2244
Size: 1109 713 347 75
Fragments arranged by size:
1109 713 347 75
Bme1580I G_kGCm'C
Cuts at: 0 190 1393 1638 2244
Size: 190 1203 245 606
Fragments arranged by size:
1203 606 245 190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 387 2244
Size: 387 1857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 351 1309 1576 1599 1776 1822 2244
Size: 244 107 958 267 23 177 46 422
Fragments arranged by size:
958 422 267 244 177 107 46 23
Bpu10I CC'TnA_GC
Cuts at: 0 806 1855 2244
Size: 806 1049 389
Fragments arranged by size:
1049 806 389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 910 2137 2244
Size: 604 306 1227 107
Fragments arranged by size:
1227 604 306 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 850 2244
Size: 358 492 1394
Fragments arranged by size:
1394 492 358
BsaAI yAC'GTr
Cuts at: 0 179 864 1748 2244
Size: 179 685 884 496
Fragments arranged by size:
884 685 496 179
BsaHI Gr'CG_yC
Cuts at: 0 83 152 428 484 539 842 957 1901
Size: 83 69 276 56 55 303 115 944
Cuts at: 1901 2244
Size: 343
Fragments arranged by size:
944 343 303 276 115 83 69 56
55
BsaWI w'CCGG_w
Cuts at: 0 1832 2244
Size: 1832 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 766 796 874 904 994 1024
Size: 592 30 144 30 78 30 90 30
Cuts at: 1024 2244
Size: 1220
Fragments arranged by size:
1220 592 144 90 78 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 1234 1540 1612 1828 1995 2149 2244
Size: 1015 219 306 72 216 167 154 95
Fragments arranged by size:
1015 306 219 216 167 154 95 72
BseYI C'CCAG_C
Cuts at: 0 53 147 166 1589 2046 2244
Size: 53 94 19 1423 457 198
Fragments arranged by size:
1423 457 198 94 53 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1975 2044 2244
Size: 72 1903 69 200
Fragments arranged by size:
1903 200 72 69
BsiEI CG_ry'CG
Cuts at: 0 569 2244
Size: 569 1675
BsiHKAI G_wGCw'C
Cuts at: 0 512 593 727 754 2153 2244
Size: 512 81 134 27 1399 91
Fragments arranged by size:
1399 512 134 91 81 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1858 2244
Size: 1858 386
Bsp1286I G_dGCh'C
Cuts at: 0 190 512 593 727 754 926 1372 1393
Size: 190 322 81 134 27 172 446 21
Cuts at: 1393 1615 1638 2018 2063 2153 2170 2244
Size: 222 23 380 45 90 17 74
Fragments arranged by size:
446 380 322 222 190 172 134 90
81 74 45 27 23 21 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5 1500 2244
Size: 5 1495 744
Fragments arranged by size:
1495 744 5
BsrBI CCG'CTC
Cuts at: 0 470 1113 1530 1719 2244
Size: 470 643 417 189 525
Fragments arranged by size:
643 525 470 417 189
BsrFI r'CCGG_y
Cuts at: 0 91 118 556 953 1998 2244
Size: 91 27 438 397 1045 246
Fragments arranged by size:
1045 438 397 246 91 27
BsrGI T'GTAC_A
Cuts at: 0 245 2244
Size: 245 1999
BssSI C'ACGA_G
Cuts at: 0 123 592 1654 2244
Size: 123 469 1062 590
Fragments arranged by size:
1062 590 469 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1962 2244
Size: 1962 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 795 1800 2244
Size: 795 1005 444
Fragments arranged by size:
1005 795 444
BstYI r'GATC_y
Cuts at: 0 614 1073 2244
Size: 614 459 1171
Fragments arranged by size:
1171 614 459
Bsu36I CC'TnA_GG
Cuts at: 0 478 2244
Size: 478 1766
BtgI C'CryG_G
Cuts at: 0 78 132 210 458 576 764 960 1451
Size: 78 54 78 248 118 188 196 491
Cuts at: 1451 1711 2009 2244
Size: 260 298 235
Fragments arranged by size:
491 298 260 248 235 196 188 118
78 78 54
BtsI GCAGTG_nn'
Cuts at: 0 1241 2244
Size: 1241 1003
EaeI y'GGCC_r
Cuts at: 0 75 455 554 566 701 767 1454 2244
Size: 75 380 99 12 135 66 687 790
Fragments arranged by size:
790 687 380 135 99 75 66 12
EagI C'GGCC_G
Cuts at: 0 566 2244
Size: 566 1678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1737 2244
Size: 1737 507
EcoICRI GAG'CTC
Cuts at: 0 725 2151 2244
Size: 725 1426 93
Fragments arranged by size:
1426 725 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 351 688 1309 1576 1599 1776 1822
Size: 244 107 337 621 267 23 177 46
Cuts at: 1822 1977 2244
Size: 155 267
Fragments arranged by size:
621 337 267 267 244 177 155 107
46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 1360 1854 2244
Size: 799 561 494 390
Fragments arranged by size:
799 561 494 390
EcoO109I rG'GnC_Cy
Cuts at: 0 38 326 383 1160 2244
Size: 38 288 57 777 1084
Fragments arranged by size:
1084 777 288 57 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2007 2244
Size: 2007 237
FseI GG_CCGG'CC
Cuts at: 0 2002 2244
Size: 2002 242
FspI TGC'GCA
Cuts at: 0 502 676 2244
Size: 502 174 1568
Fragments arranged by size:
1568 502 174
HaeII r_GCGC'y
Cuts at: 0 210 282 529 651 915 960 1904 2176
Size: 210 72 247 122 264 45 944 272
Cuts at: 2176 2244
Size: 68
Fragments arranged by size:
944 272 264 247 210 122 72 68
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 766 798 874 906 1324 1356
Size: 22 32 712 32 76 32 418 32
Cuts at: 1356 2140 2172 2244
Size: 784 32 72
Fragments arranged by size:
784 712 418 76 72 32 32 32
32 32 22
KasI G'GCGC_C
Cuts at: 0 956 1900 2244
Size: 956 944 344
Fragments arranged by size:
956 944 344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1427 2153 2244
Size: 662 765 726 91
Fragments arranged by size:
765 726 662 91
MscI TGG'CCA
Cuts at: 0 703 2244
Size: 703 1541
MslI CAynn'nnrTG
Cuts at: 0 77 1333 1546 2244
Size: 77 1256 213 698
Fragments arranged by size:
1256 698 213 77
MspA1I CmG'CkG
Cuts at: 0 170 460 578 1108 1453 1589 1631 1713
Size: 170 290 118 530 345 136 42 82
Cuts at: 1713 2011 2244
Size: 298 233
Fragments arranged by size:
530 345 298 290 233 170 136 118
82 42
NaeI GCC'GGC
Cuts at: 0 558 2000 2244
Size: 558 1442 244
Fragments arranged by size:
1442 558 244
NarI GG'CG_CC
Cuts at: 0 957 1901 2244
Size: 957 944 343
Fragments arranged by size:
957 944 343
NcoI C'CATG_G
Cuts at: 0 78 960 2244
Size: 78 882 1284
Fragments arranged by size:
1284 882 78
NgoMIV G'CCGG_C
Cuts at: 0 556 1998 2244
Size: 556 1442 246
Fragments arranged by size:
1442 556 246
NspI r_CATG'y
Cuts at: 0 414 2244
Size: 414 1830
PfoI T'CCnGG_A
Cuts at: 0 983 1156 1553 2244
Size: 983 173 397 691
Fragments arranged by size:
983 691 397 173
PmlI CAC'GTG
Cuts at: 0 1748 2244
Size: 1748 496
PpuMI rG'GwC_Cy
Cuts at: 0 38 326 383 1160 2244
Size: 38 288 57 777 1084
Fragments arranged by size:
1084 777 288 57 38
PshAI GACnn'nnGTC
Cuts at: 0 1965 2244
Size: 1965 279
PspXI vC'TCGA_Gb
Cuts at: 0 1933 2244
Size: 1933 311
PstI C_TGCA'G
Cuts at: 0 347 1361 1433 1811 2244
Size: 347 1014 72 378 433
Fragments arranged by size:
1014 433 378 347 72
PvuII CAG'CTG
Cuts at: 0 1589 2244
Size: 1589 655
SacI G_AGCT'C
Cuts at: 0 727 2153 2244
Size: 727 1426 91
Fragments arranged by size:
1426 727 91
SacII CC_GC'GG
Cuts at: 0 461 579 1454 1714 2012 2244
Size: 461 118 875 260 298 232
Fragments arranged by size:
875 461 298 260 232 118
SfcI C'TryA_G
Cuts at: 0 343 1357 1429 1807 1887 2244
Size: 343 1014 72 378 80 357
Fragments arranged by size:
1014 378 357 343 80 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 101 2244
Size: 101 2143
SfoI GGC'GCC
Cuts at: 0 958 1902 2244
Size: 958 944 342
Fragments arranged by size:
958 944 342
SmaI CCC'GGG
Cuts at: 0 1698 2244
Size: 1698 546
SmlI C'TyrA_G
Cuts at: 0 619 925 1503 1933 2152 2244
Size: 619 306 578 430 219 92
Fragments arranged by size:
619 578 430 306 219 92
SphI G_CATG'C
Cuts at: 0 414 2244
Size: 414 1830
StyI C'CwwG_G
Cuts at: 0 78 98 105 960 995 1970 2244
Size: 78 20 7 855 35 975 274
Fragments arranged by size:
975 855 274 78 35 20 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 376 2244
Size: 231 145 1868
Fragments arranged by size:
1868 231 145
TatI w'GTAC_w
Cuts at: 0 245 1010 2244
Size: 245 765 1234
Fragments arranged by size:
1234 765 245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 2244
Size: 592 32 1620
Fragments arranged by size:
1620 592 32
Tth111I GACn'n_nGTC
Cuts at: 0 1728 2244
Size: 1728 516
XbaI T'CTAG_A
Cuts at: 0 2121 2244
Size: 2121 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 403 696 792 2244
Size: 403 293 96 1452
Fragments arranged by size:
1452 403 293 96
XhoI C'TCGA_G
Cuts at: 0 1503 1933 2244
Size: 1503 430 311
Fragments arranged by size:
1503 430 311
XmaI C'CCGG_G
Cuts at: 0 1696 2244
Size: 1696 548
ZraI GAC'GTC
Cuts at: 0 485 2244
Size: 485 1759
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AlwNI AvaI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PfoI PmlI PpuMI PshAI PspXI PstI
PvuII SacI SacII SfcI SfiI SfoI SmaI SmlI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BsaBI BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EcoRI EcoRV FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PsiI PspOMI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
XmnI