Restriction map for
pFN21AE6713
(Linear) (Six-base) MAPSORT of: KIEE6713.seq Check: 9326 from: 1 to: 2163
KIEE6713.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 335 2163
Size: 335 1828
Acc65I G'GTAC_C
Cuts at: 0 704 1754 2163
Size: 704 1050 409
Fragments arranged by size:
1050 704 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1671 1734 2163
Size: 1671 63 429
Fragments arranged by size:
1671 429 63
AfeI AGC'GCT
Cuts at: 0 979 2163
Size: 979 1184
AflIII A'CryG_T
Cuts at: 0 162 2163
Size: 162 2001
AleI CACnn'nnGTG
Cuts at: 0 343 769 1300 2163
Size: 343 426 531 863
Fragments arranged by size:
863 531 426 343
AlwNI CAG_nnn'CTG
Cuts at: 0 737 930 1176 1355 1413 2163
Size: 737 193 246 179 58 750
Fragments arranged by size:
750 737 246 193 179 58
ApaI G_GGCC'C
Cuts at: 0 132 645 1490 2163
Size: 132 513 845 673
Fragments arranged by size:
845 673 513 132
ApaLI G'TGCA_C
Cuts at: 0 1234 2163
Size: 1234 929
ApoI r'AATT_y
Cuts at: 0 1060 2074 2163
Size: 1060 1014 89
Fragments arranged by size:
1060 1014 89
AvaI C'yCGr_G
Cuts at: 0 8 20 132 213 226 912 1769 1972
Size: 8 12 112 81 13 686 857 203
Cuts at: 1972 2156 2163
Size: 184 7
Fragments arranged by size:
857 686 203 184 112 81 13 12
8 7
BamHI G'GATC_C
Cuts at: 0 506 2163
Size: 506 1657
BanI G'GyrC_C
Cuts at: 0 14 447 704 1656 1754 1863 2163
Size: 14 433 257 952 98 109 300
Fragments arranged by size:
952 433 300 257 109 98 14
BanII G_rGCy'C
Cuts at: 0 132 287 638 645 848 1124 1490 1961
Size: 132 155 351 7 203 276 366 471
Cuts at: 1961 2152 2163
Size: 191 11
Fragments arranged by size:
471 366 351 276 203 191 155 132
11 7
BbeI G_GCGC'C
Cuts at: 0 18 2163
Size: 18 2145
BbsI GAAGACnn'nnnn_
Cuts at: 0 1202 1542 2163
Size: 1202 340 621
Fragments arranged by size:
1202 621 340
BclI T'GATC_A
Cuts at: 0 899 2163
Size: 899 1264
BglI GCCn_nnn'nGGC
Cuts at: 0 12 117 2163
Size: 12 105 2046
Fragments arranged by size:
2046 105 12
BlpI GC'TnA_GC
Cuts at: 0 575 1157 1403 2163
Size: 575 582 246 760
Fragments arranged by size:
760 582 575 246
Bme1580I G_kGCm'C
Cuts at: 0 132 645 1135 1238 1490 1738 2163
Size: 132 513 490 103 252 248 425
Fragments arranged by size:
513 490 425 252 248 132 103
BmgBI CAC'GTC
Cuts at: 0 1458 2163
Size: 1458 705
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 2163
Size: 554 1609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 442 1111 2016 2041 2163
Size: 306 136 669 905 25 122
Fragments arranged by size:
905 669 306 136 122 25
Bpu10I CC'TnA_GC
Cuts at: 0 850 1811 2163
Size: 850 961 352
Fragments arranged by size:
961 850 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 988 2050 2163
Size: 472 516 1062 113
Fragments arranged by size:
1062 516 472 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 203 504 1663 2163
Size: 203 301 1159 500
Fragments arranged by size:
1159 500 301 203
BsaAI yAC'GTr
Cuts at: 0 163 1857 2163
Size: 163 1694 306
Fragments arranged by size:
1694 306 163
BsaHI Gr'CG_yC
Cuts at: 0 15 332 401 994 2163
Size: 15 317 69 593 1169
Fragments arranged by size:
1169 593 317 69 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 368 398 805 835 2163
Size: 226 30 112 30 407 30 1328
Fragments arranged by size:
1328 407 226 112 30 30 30
BseYI C'CCAG_C
Cuts at: 0 27 809 1135 1307 1620 1626 2127 2163
Size: 27 782 326 172 313 6 501 36
Fragments arranged by size:
782 501 326 313 172 36 27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 943 970 1368 2163
Size: 364 579 27 398 795
Fragments arranged by size:
795 579 398 364 27
BsiEI CG_ry'CG
Cuts at: 0 677 2163
Size: 677 1486
BsiHKAI G_wGCw'C
Cuts at: 0 287 638 848 911 1124 1238 1961 2163
Size: 287 351 210 63 213 114 723 202
Fragments arranged by size:
723 351 287 213 210 202 114 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 323 418 2163
Size: 323 95 1745
Fragments arranged by size:
1745 323 95
Bsp1286I G_dGCh'C
Cuts at: 0 132 287 638 645 848 911 1124 1135
Size: 132 155 351 7 203 63 213 11
Cuts at: 1135 1238 1490 1738 1961 2152 2163
Size: 103 252 248 223 191 11
Fragments arranged by size:
351 252 248 223 213 203 191 155
132 103 63 11 11 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1115 2163
Size: 1115 1048
BsrBI CCG'CTC
Cuts at: 0 124 153 242 2030 2163
Size: 124 29 89 1788 133
Fragments arranged by size:
1788 133 124 89 29
BsrDI GCAATG_nn'
Cuts at: 0 1837 2163
Size: 1837 326
BsrFI r'CCGG_y
Cuts at: 0 220 791 1322 1634 1659 2163
Size: 220 571 531 312 25 504
Fragments arranged by size:
571 531 504 312 220 25
BssSI C'ACGA_G
Cuts at: 0 1759 1868 2163
Size: 1759 109 295
Fragments arranged by size:
1759 295 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 930 1242 1413 2163
Size: 930 312 171 750
Fragments arranged by size:
930 750 312 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1374 2163
Size: 870 504 789
Fragments arranged by size:
870 789 504
BstYI r'GATC_y
Cuts at: 0 506 2163
Size: 506 1657
Bsu36I CC'TnA_GG
Cuts at: 0 1600 2163
Size: 1600 563
BtgI C'CryG_G
Cuts at: 0 404 1316 2100 2163
Size: 404 912 784 63
Fragments arranged by size:
912 784 404 63
BtsI GCAGTG_nn'
Cuts at: 0 376 2163
Size: 376 1787
DraIII CAC_nnn'GTG
Cuts at: 0 393 1153 1242 1932 2163
Size: 393 760 89 690 231
Fragments arranged by size:
760 690 393 231 89
DrdI GACnn_nn'nnGTC
Cuts at: 0 607 2163
Size: 607 1556
EaeI y'GGCC_r
Cuts at: 0 296 407 971 1838 2163
Size: 296 111 564 867 325
Fragments arranged by size:
867 564 325 296 111
EarI CTCTTCn'nnn_
Cuts at: 0 364 436 2163
Size: 364 72 1727
Fragments arranged by size:
1727 364 72
EcoICRI GAG'CTC
Cuts at: 0 285 636 846 1122 1959 2163
Size: 285 351 210 276 837 204
Fragments arranged by size:
837 351 285 276 210 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 442 1111 1671 1734 2016 2041 2163
Size: 306 136 669 560 63 282 25 122
Fragments arranged by size:
669 560 306 282 136 122 63 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2156 2163
Size: 2156 7
EcoO109I rG'GnC_Cy
Cuts at: 0 87 128 129 642 734 1173 1486 1487
Size: 87 41 1 513 92 439 313 1
Cuts at: 1487 1533 1604 1815 2061 2163
Size: 46 71 211 246 102
Fragments arranged by size:
513 439 313 246 211 102 92 87
71 46 41 1 1
EcoRI G'AATT_C
Cuts at: 0 1060 2074 2163
Size: 1060 1014 89
Fragments arranged by size:
1060 1014 89
FseI GG_CCGG'CC
Cuts at: 0 224 2163
Size: 224 1939
HaeII r_GCGC'y
Cuts at: 0 18 28 105 981 2163
Size: 18 10 77 876 1182
Fragments arranged by size:
1182 876 77 18 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 320 352 2163
Size: 320 32 1811
Fragments arranged by size:
1811 320 32
HincII GTy'rAC
Cuts at: 0 1503 2163
Size: 1503 660
HindIII A'AGCT_T
Cuts at: 0 1033 2163
Size: 1033 1130
KasI G'GCGC_C
Cuts at: 0 14 2163
Size: 14 2149
KpnI G_GTAC'C
Cuts at: 0 708 1758 2163
Size: 708 1050 405
Fragments arranged by size:
1050 708 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1316 2163
Size: 269 1047 847
Fragments arranged by size:
1047 847 269
MscI TGG'CCA
Cuts at: 0 298 409 2163
Size: 298 111 1754
Fragments arranged by size:
1754 298 111
MslI CAynn'nnrTG
Cuts at: 0 343 442 769 1300 2163
Size: 343 99 327 531 863
Fragments arranged by size:
863 531 343 327 99
MspA1I CmG'CkG
Cuts at: 0 954 1311 1620 1799 2163
Size: 954 357 309 179 364
Fragments arranged by size:
954 364 357 309 179
NaeI GCC'GGC
Cuts at: 0 222 1636 1661 2163
Size: 222 1414 25 502
Fragments arranged by size:
1414 502 222 25
NarI GG'CG_CC
Cuts at: 0 15 2163
Size: 15 2148
NcoI C'CATG_G
Cuts at: 0 1316 2100 2163
Size: 1316 784 63
Fragments arranged by size:
1316 784 63
NgoMIV G'CCGG_C
Cuts at: 0 220 1634 1659 2163
Size: 220 1414 25 504
Fragments arranged by size:
1414 504 220 25
NspI r_CATG'y
Cuts at: 0 295 1057 1249 2163
Size: 295 762 192 914
Fragments arranged by size:
914 762 295 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 1932 2163
Size: 1932 231
PfoI T'CCnGG_A
Cuts at: 0 419 502 596 1088 2163
Size: 419 83 94 492 1075
Fragments arranged by size:
1075 492 419 94 83
PmlI CAC'GTG
Cuts at: 0 163 2163
Size: 163 2000
PpuMI rG'GwC_Cy
Cuts at: 0 87 2163
Size: 87 2076
PspOMI G'GGCC_C
Cuts at: 0 128 641 1486 2163
Size: 128 513 845 677
Fragments arranged by size:
845 677 513 128
PstI C_TGCA'G
Cuts at: 0 1331 2138 2163
Size: 1331 807 25
Fragments arranged by size:
1331 807 25
PvuI CG_AT'CG
Cuts at: 0 677 2163
Size: 677 1486
PvuII CAG'CTG
Cuts at: 0 954 1311 1620 2163
Size: 954 357 309 543
Fragments arranged by size:
954 543 357 309
SacI G_AGCT'C
Cuts at: 0 287 638 848 1124 1961 2163
Size: 287 351 210 276 837 202
Fragments arranged by size:
837 351 287 276 210 202
SapI GCTCTTCn'nnn_
Cuts at: 0 364 436 2163
Size: 364 72 1727
Fragments arranged by size:
1727 364 72
SexAI A'CCwGG_T
Cuts at: 0 1149 2163
Size: 1149 1014
SfcI C'TryA_G
Cuts at: 0 363 1327 2134 2163
Size: 363 964 807 29
Fragments arranged by size:
964 807 363 29
SfoI GGC'GCC
Cuts at: 0 16 2163
Size: 16 2147
SmaI CCC'GGG
Cuts at: 0 22 134 215 228 914 2163
Size: 22 112 81 13 686 1249
Fragments arranged by size:
1249 686 112 81 22 13
SmlI C'TyrA_G
Cuts at: 0 487 1003 2065 2163
Size: 487 516 1062 98
Fragments arranged by size:
1062 516 487 98
StuI AGG'CCT
Cuts at: 0 1429 1978 2163
Size: 1429 549 185
Fragments arranged by size:
1429 549 185
StyI C'CwwG_G
Cuts at: 0 746 1316 1523 1703 2100 2163
Size: 746 570 207 180 397 63
Fragments arranged by size:
746 570 397 207 180 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 1797 2163
Size: 667 1130 366
Fragments arranged by size:
1130 667 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 226 258 368 400 805 837
Size: 79 32 115 32 110 32 405 32
Cuts at: 837 1330 1362 2163
Size: 493 32 801
Fragments arranged by size:
801 493 405 115 110 79 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 1147 1617 2163
Size: 417 730 470 546
Fragments arranged by size:
730 546 470 417
XmaI C'CCGG_G
Cuts at: 0 20 132 213 226 912 2163
Size: 20 112 81 13 686 1251
Fragments arranged by size:
1251 686 112 81 20 13
ZraI GAC'GTC
Cuts at: 0 333 2163
Size: 333 1830
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuI PvuII SacI
SapI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BfrBI BglII BmtI BplI BsaBI BsaWI BseRI BsiWI
BsmI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EagI EciI EcoRV FalI
FspI FspAI HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmnI