Restriction map for
pFN21AE6708
(Linear) (Six-base) MAPSORT of: KIEE6708.seq Check: 7662 from: 1 to: 2217
KIEE6708.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1223 1365 2217
Size: 1223 142 852
Fragments arranged by size:
1223 852 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 769 1213 1735 2217
Size: 502 267 444 522 482
Fragments arranged by size:
522 502 482 444 267
AfeI AGC'GCT
Cuts at: 0 1462 2217
Size: 1462 755
AflIII A'CryG_T
Cuts at: 0 701 1494 2217
Size: 701 793 723
Fragments arranged by size:
793 723 701
AlwNI CAG_nnn'CTG
Cuts at: 0 1203 2217
Size: 1203 1014
ApaI G_GGCC'C
Cuts at: 0 1158 2217
Size: 1158 1059
AvaI C'yCGr_G
Cuts at: 0 229 1253 2217
Size: 229 1024 964
Fragments arranged by size:
1024 964 229
BamHI G'GATC_C
Cuts at: 0 93 2217
Size: 93 2124
BanI G'GyrC_C
Cuts at: 0 36 627 651 1015 2217
Size: 36 591 24 364 1202
Fragments arranged by size:
1202 591 364 36 24
BanII G_rGCy'C
Cuts at: 0 222 1158 1490 1958 2217
Size: 222 936 332 468 259
Fragments arranged by size:
936 468 332 259 222
BbeI G_GCGC'C
Cuts at: 0 40 2217
Size: 40 2177
BbsI GAAGACnn'nnnn_
Cuts at: 0 1178 2217
Size: 1178 1039
BbvCI CC'TCA_GC
Cuts at: 0 1088 1642 2217
Size: 1088 554 575
Fragments arranged by size:
1088 575 554
BlpI GC'TnA_GC
Cuts at: 0 1952 2217
Size: 1952 265
Bme1580I G_kGCm'C
Cuts at: 0 1143 1158 2217
Size: 1143 15 1059
Fragments arranged by size:
1143 1059 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 421 775 793 1018 1268 1417 1615
Size: 177 244 354 18 225 250 149 198
Cuts at: 1615 1726 1897 2217
Size: 111 171 320
Fragments arranged by size:
354 320 250 244 225 198 177 171
149 111 18
Bpu10I CC'TnA_GC
Cuts at: 0 1088 1642 2217
Size: 1088 554 575
Fragments arranged by size:
1088 575 554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 718 1282 2217
Size: 355 363 564 935
Fragments arranged by size:
935 564 363 355
BsaI GGTCTCn'nnnn_
Cuts at: 0 1561 2217
Size: 1561 656
BsaAI yAC'GTr
Cuts at: 0 1794 2217
Size: 1794 423
BsaHI Gr'CG_yC
Cuts at: 0 4 37 1163 2217
Size: 4 33 1126 1054
Fragments arranged by size:
1126 1054 33 4
BsaWI w'CCGG_w
Cuts at: 0 599 2217
Size: 599 1618
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 991 1021 1266 1296 2217
Size: 175 30 786 30 245 30 921
Fragments arranged by size:
921 786 245 175 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 754 820 1429 1714 2164 2176 2217
Size: 33 721 66 609 285 450 12 41
Fragments arranged by size:
721 609 450 285 66 41 33 12
BseYI C'CCAG_C
Cuts at: 0 898 2217
Size: 898 1319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1195 1747 1855 2217
Size: 147 1048 552 108 362
Fragments arranged by size:
1048 552 362 147 108
BsiEI CG_ry'CG
Cuts at: 0 33 970 2217
Size: 33 937 1247
Fragments arranged by size:
1247 937 33
BsiHKAI G_wGCw'C
Cuts at: 0 1439 1958 2217
Size: 1439 519 259
Fragments arranged by size:
1439 519 259
Bsp1286I G_dGCh'C
Cuts at: 0 222 1143 1158 1439 1490 1958 2217
Size: 222 921 15 281 51 468 259
Fragments arranged by size:
921 468 281 259 222 51 15
BspEI T'CCGG_A
Cuts at: 0 599 2217
Size: 599 1618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1217 1223 1365 2217
Size: 1217 6 142 852
Fragments arranged by size:
1217 852 142 6
BsrDI GCAATG_nn'
Cuts at: 0 909 2217
Size: 909 1308
BsrGI T'GTAC_A
Cuts at: 0 182 419 2217
Size: 182 237 1798
Fragments arranged by size:
1798 237 182
BssSI C'ACGA_G
Cuts at: 0 1142 2217
Size: 1142 1075
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 2217
Size: 468 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 1894 2118 2217
Size: 123 1771 224 99
Fragments arranged by size:
1771 224 123 99
BstYI r'GATC_y
Cuts at: 0 93 602 875 1226 2217
Size: 93 509 273 351 991
Fragments arranged by size:
991 509 351 273 93
Bsu36I CC'TnA_GG
Cuts at: 0 1180 2217
Size: 1180 1037
BtgI C'CryG_G
Cuts at: 0 27 76 259 495 1925 2217
Size: 27 49 183 236 1430 292
Fragments arranged by size:
1430 292 236 183 49 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 359 1220 1661 2217
Size: 359 861 441 556
Fragments arranged by size:
861 556 441 359
BtsI GCAGTG_nn'
Cuts at: 0 1204 1306 1580 2217
Size: 1204 102 274 637
Fragments arranged by size:
1204 637 274 102
ClaI AT'CG_AT
Cuts at: 0 1856 2217
Size: 1856 361
DraIII CAC_nnn'GTG
Cuts at: 0 180 2217
Size: 180 2037
DrdI GACnn_nn'nnGTC
Cuts at: 0 2062 2217
Size: 2062 155
EaeI y'GGCC_r
Cuts at: 0 30 561 779 967 1355 1421 1910 2012
Size: 30 531 218 188 388 66 489 102
Cuts at: 2012 2217
Size: 205
Fragments arranged by size:
531 489 388 218 205 188 102 66
30
EagI C'GGCC_G
Cuts at: 0 30 967 2217
Size: 30 937 1250
Fragments arranged by size:
1250 937 30
EarI CTCTTCn'nnn_
Cuts at: 0 333 622 697 1607 2206 2217
Size: 333 289 75 910 599 11
Fragments arranged by size:
910 599 333 289 75 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 610 2217
Size: 493 117 1607
Fragments arranged by size:
1607 493 117
EcoICRI GAG'CTC
Cuts at: 0 1956 2217
Size: 1956 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 421 502 769 775 793 1018 1213
Size: 177 244 81 267 6 18 225 195
Cuts at: 1213 1268 1417 1615 1726 1735 1897 2217
Size: 55 149 198 111 9 162 320
Fragments arranged by size:
320 267 244 225 198 195 177 162
149 111 81 55 18 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1219 2065 2217
Size: 1219 846 152
Fragments arranged by size:
1219 846 152
EcoO109I rG'GnC_Cy
Cuts at: 0 295 1155 2217
Size: 295 860 1062
Fragments arranged by size:
1062 860 295
HaeII r_GCGC'y
Cuts at: 0 40 259 845 1464 2217
Size: 40 219 586 619 753
Fragments arranged by size:
753 619 586 219 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 991 1023 1264 1296 1639 1671
Size: 712 32 247 32 241 32 343 32
Cuts at: 1671 2217
Size: 546
Fragments arranged by size:
712 546 343 247 241 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2049 2217
Size: 2049 168
KasI G'GCGC_C
Cuts at: 0 36 2217
Size: 36 2181
MscI TGG'CCA
Cuts at: 0 781 2217
Size: 781 1436
MspA1I CmG'CkG
Cuts at: 0 29 78 110 465 975 1191 1875 2217
Size: 29 49 32 355 510 216 684 342
Fragments arranged by size:
684 510 355 342 216 49 32 29
NarI GG'CG_CC
Cuts at: 0 37 2217
Size: 37 2180
NcoI C'CATG_G
Cuts at: 0 259 495 2217
Size: 259 236 1722
Fragments arranged by size:
1722 259 236
NruI TCG'CGA
Cuts at: 0 344 2217
Size: 344 1873
NspI r_CATG'y
Cuts at: 0 543 705 1152 1923 2217
Size: 543 162 447 771 294
Fragments arranged by size:
771 543 447 294 162
PasI CC'CwG_GG
Cuts at: 0 123 456 1158 2217
Size: 123 333 702 1059
Fragments arranged by size:
1059 702 333 123
PciI A'CATG_T
Cuts at: 0 701 2217
Size: 701 1516
PfoI T'CCnGG_A
Cuts at: 0 791 878 1268 2217
Size: 791 87 390 949
Fragments arranged by size:
949 791 390 87
PpuMI rG'GwC_Cy
Cuts at: 0 295 2217
Size: 295 1922
PspOMI G'GGCC_C
Cuts at: 0 1154 2217
Size: 1154 1063
PstI C_TGCA'G
Cuts at: 0 1127 1313 1412 1457 1562 1667 2217
Size: 1127 186 99 45 105 105 550
Fragments arranged by size:
1127 550 186 105 105 99 45
PvuII CAG'CTG
Cuts at: 0 110 975 1191 1875 2217
Size: 110 865 216 684 342
Fragments arranged by size:
865 684 342 216 110
SacI G_AGCT'C
Cuts at: 0 1958 2217
Size: 1958 259
SacII CC_GC'GG
Cuts at: 0 30 79 2217
Size: 30 49 2138
Fragments arranged by size:
2138 49 30
SapI GCTCTTCn'nnn_
Cuts at: 0 697 2206 2217
Size: 697 1509 11
Fragments arranged by size:
1509 697 11
SfcI C'TryA_G
Cuts at: 0 1003 1123 1309 1408 1453 1558 1663 1734
Size: 1003 120 186 99 45 105 105 71
Cuts at: 1734 2217
Size: 483
Fragments arranged by size:
1003 483 186 120 105 105 99 71
45
SfoI GGC'GCC
Cuts at: 0 38 2217
Size: 38 2179
SmaI CCC'GGG
Cuts at: 0 1255 2217
Size: 1255 962
SmlI C'TyrA_G
Cuts at: 0 370 733 1297 2217
Size: 370 363 564 920
Fragments arranged by size:
920 564 370 363
SphI G_CATG'C
Cuts at: 0 543 1152 1923 2217
Size: 543 609 771 294
Fragments arranged by size:
771 609 543 294
StyI C'CwwG_G
Cuts at: 0 259 495 833 1688 2217
Size: 259 236 338 855 529
Fragments arranged by size:
855 529 338 259 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1540 2217
Size: 1540 677
TatI w'GTAC_w
Cuts at: 0 182 419 2217
Size: 182 237 1798
Fragments arranged by size:
1798 237 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1266 1298 2217
Size: 1266 32 919
Fragments arranged by size:
1266 919 32
Tth111I GACn'n_nGTC
Cuts at: 0 726 1552 2062 2217
Size: 726 826 510 155
Fragments arranged by size:
826 726 510 155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 807 2115 2217
Size: 807 1308 102
Fragments arranged by size:
1308 807 102
XmaI C'CCGG_G
Cuts at: 0 1253 2217
Size: 1253 964
XmnI GAAnn'nnTTC
Cuts at: 0 691 2096 2217
Size: 691 1405 121
Fragments arranged by size:
1405 691 121
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MscI MspA1I NarI NcoI NruI NspI PasI PciI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SmaI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BsaBI BsiWI BsmI BsmBI BspHI
BsrBI BsrFI BssHII BstBI BstEII BstZ17I CspCI DraI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV
NheI NotI NsiI PacI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XhoI ZraI