Restriction map for
pFN21AE6702
(Linear) (Six-base) MAPSORT of: KIEE6702.seq Check: 3628 from: 1 to: 2274
KIEE6702.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1542 2274
Size: 1542 732
AccI GT'mk_AC
Cuts at: 0 1706 2274
Size: 1706 568
AclI AA'CG_TT
Cuts at: 0 1871 2274
Size: 1871 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1702 1777 1783 1990 2083 2274
Size: 1378 324 75 6 207 93 191
Fragments arranged by size:
1378 324 207 191 93 75 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2207 2274
Size: 2207 67
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 626 658 2274
Size: 626 32 1616
Fragments arranged by size:
1616 626 32
AlwNI CAG_nnn'CTG
Cuts at: 0 42 870 1137 2274
Size: 42 828 267 1137
Fragments arranged by size:
1137 828 267 42
AseI AT'TA_AT
Cuts at: 0 1832 2274
Size: 1832 442
AvaI C'yCGr_G
Cuts at: 0 194 536 886 2232 2274
Size: 194 342 350 1346 42
Fragments arranged by size:
1346 350 342 194 42
AvrII C'CTAG_G
Cuts at: 0 151 2274
Size: 151 2123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 232 265 2274
Size: 232 33 2009
Fragments arranged by size:
2009 232 33
BanI G'GyrC_C
Cuts at: 0 793 990 1554 1816 2023 2274
Size: 793 197 564 262 207 251
Fragments arranged by size:
793 564 262 251 207 197
BanII G_rGCy'C
Cuts at: 0 145 1900 2274
Size: 145 1755 374
Fragments arranged by size:
1755 374 145
BbeI G_GCGC'C
Cuts at: 0 1820 2274
Size: 1820 454
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 669 1617 1859 1946 2274
Size: 381 288 948 242 87 328
Fragments arranged by size:
948 381 328 288 242 87
BbvCI CC'TCA_GC
Cuts at: 0 1369 1988 2274
Size: 1369 619 286
Fragments arranged by size:
1369 619 286
BciVI GTATCCnnnnn_n'
Cuts at: 0 260 2274
Size: 260 2014
BclI T'GATC_A
Cuts at: 0 509 2274
Size: 509 1765
BfrBI ATG'CAT
Cuts at: 0 2167 2274
Size: 2167 107
BglI GCCn_nnn'nGGC
Cuts at: 0 340 490 1411 2274
Size: 340 150 921 863
Fragments arranged by size:
921 863 340 150
BglII A'GATC_T
Cuts at: 0 1091 2274
Size: 1091 1183
BlpI GC'TnA_GC
Cuts at: 0 144 1378 2274
Size: 144 1234 896
Fragments arranged by size:
1234 896 144
Bme1580I G_kGCm'C
Cuts at: 0 995 2028 2156 2274
Size: 995 1033 128 118
Fragments arranged by size:
1033 995 128 118
BmrI ACTGGGnnnn_n'
Cuts at: 0 991 1093 2274
Size: 991 102 1181
Fragments arranged by size:
1181 991 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1274 2274
Size: 778 496 1000
Fragments arranged by size:
1000 778 496
Bpu10I CC'TnA_GC
Cuts at: 0 1369 1899 1988 2274
Size: 1369 530 89 286
Fragments arranged by size:
1369 530 286 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1723 2038 2164 2274
Size: 676 1047 315 126 110
Fragments arranged by size:
1047 676 315 126 110
BsaHI Gr'CG_yC
Cuts at: 0 1817 2274
Size: 1817 457
BsaWI w'CCGG_w
Cuts at: 0 1517 2274
Size: 1517 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1375 1405 2274
Size: 1375 30 869
Fragments arranged by size:
1375 869 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 1270 1623 1795 1875 2274
Size: 139 1131 353 172 80 399
Fragments arranged by size:
1131 399 353 172 139 80
BseYI C'CCAG_C
Cuts at: 0 129 137 591 817 1013 2149 2274
Size: 129 8 454 226 196 1136 125
Fragments arranged by size:
1136 454 226 196 129 125 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 2274
Size: 691 1583
BsiEI CG_ry'CG
Cuts at: 0 100 2274
Size: 100 2174
BsiHKAI G_wGCw'C
Cuts at: 0 187 195 830 1231 1900 2274
Size: 187 8 635 401 669 374
Fragments arranged by size:
669 635 401 374 187 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 687 2274
Size: 687 1587
Bsp1286I G_dGCh'C
Cuts at: 0 145 187 195 830 995 1231 1900 2028
Size: 145 42 8 635 165 236 669 128
Cuts at: 2028 2156 2274
Size: 128 118
Fragments arranged by size:
669 635 236 165 145 128 128 118
42 8
BspEI T'CCGG_A
Cuts at: 0 1517 2274
Size: 1517 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 663 1236 1325 1542 2274
Size: 77 586 573 89 217 732
Fragments arranged by size:
732 586 573 217 89 77
BsrBI CCG'CTC
Cuts at: 0 1631 2274
Size: 1631 643
BsrDI GCAATG_nn'
Cuts at: 0 68 1070 2274
Size: 68 1002 1204
Fragments arranged by size:
1204 1002 68
BsrGI T'GTAC_A
Cuts at: 0 2105 2274
Size: 2105 169
BssSI C'ACGA_G
Cuts at: 0 829 1030 1507 2274
Size: 829 201 477 767
Fragments arranged by size:
829 767 477 201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 870 2274
Size: 870 1404
BstEII G'GTnAC_C
Cuts at: 0 239 274 618 1434 2262 2274
Size: 239 35 344 816 828 12
Fragments arranged by size:
828 816 344 239 35 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1056 1083 2274
Size: 1056 27 1191
Fragments arranged by size:
1191 1056 27
BstYI r'GATC_y
Cuts at: 0 1091 1416 2274
Size: 1091 325 858
Fragments arranged by size:
1091 858 325
Bsu36I CC'TnA_GG
Cuts at: 0 1102 2274
Size: 1102 1172
BtgI C'CryG_G
Cuts at: 0 428 797 2274
Size: 428 369 1477
Fragments arranged by size:
1477 428 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1035 2274
Size: 1035 1239
BtsI GCAGTG_nn'
Cuts at: 0 40 187 471 803 2274
Size: 40 147 284 332 1471
Fragments arranged by size:
1471 332 284 147 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1013 1048 2274
Size: 1013 35 1226
Fragments arranged by size:
1226 1013 35
DraIII CAC_nnn'GTG
Cuts at: 0 562 2048 2274
Size: 562 1486 226
Fragments arranged by size:
1486 562 226
EaeI y'GGCC_r
Cuts at: 0 97 299 1445 1637 2274
Size: 97 202 1146 192 637
Fragments arranged by size:
1146 637 202 192 97
EagI C'GGCC_G
Cuts at: 0 97 2274
Size: 97 2177
EarI CTCTTCn'nnn_
Cuts at: 0 583 1269 1312 2274
Size: 583 686 43 962
Fragments arranged by size:
962 686 583 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 183 1429 2274
Size: 25 158 1246 845
Fragments arranged by size:
1246 845 158 25
EcoICRI GAG'CTC
Cuts at: 0 1898 2274
Size: 1898 376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1274 1378 1702 1777 1783 1990 2083
Size: 778 496 104 324 75 6 207 93
Cuts at: 2083 2274
Size: 191
Fragments arranged by size:
778 496 324 207 191 104 93 75
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 1219 1321 1399 1549 2274
Size: 580 639 102 78 150 725
Fragments arranged by size:
725 639 580 150 102 78
EcoO109I rG'GnC_Cy
Cuts at: 0 155 434 1950 1974 2274
Size: 155 279 1516 24 300
Fragments arranged by size:
1516 300 279 155 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2205 2274
Size: 2205 69
HaeII r_GCGC'y
Cuts at: 0 138 1043 1820 2274
Size: 138 905 777 454
Fragments arranged by size:
905 777 454 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 1375 1407 2274
Size: 268 32 1075 32 867
Fragments arranged by size:
1075 867 268 32 32
HincII GTy'rAC
Cuts at: 0 1707 1839 2274
Size: 1707 132 435
Fragments arranged by size:
1707 435 132
HindIII A'AGCT_T
Cuts at: 0 1786 2274
Size: 1786 488
KasI G'GCGC_C
Cuts at: 0 1816 2274
Size: 1816 458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 2250 2274
Size: 240 2010 24
Fragments arranged by size:
2010 240 24
MscI TGG'CCA
Cuts at: 0 301 2274
Size: 301 1973
MslI CAynn'nnrTG
Cuts at: 0 427 451 1054 1348 2274
Size: 427 24 603 294 926
Fragments arranged by size:
926 603 427 294 24
MspA1I CmG'CkG
Cuts at: 0 102 661 667 811 870 1017 1755 2274
Size: 102 559 6 144 59 147 738 519
Fragments arranged by size:
738 559 519 147 144 102 59 6
NarI GG'CG_CC
Cuts at: 0 1817 2274
Size: 1817 457
NsiI A_TGCA'T
Cuts at: 0 2169 2274
Size: 2169 105
NspI r_CATG'y
Cuts at: 0 1368 1389 2274
Size: 1368 21 885
Fragments arranged by size:
1368 885 21
PasI CC'CwG_GG
Cuts at: 0 1146 1977 2274
Size: 1146 831 297
Fragments arranged by size:
1146 831 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 1137 2274
Size: 1137 1137
PfoI T'CCnGG_A
Cuts at: 0 2012 2084 2274
Size: 2012 72 190
Fragments arranged by size:
2012 190 72
PpuMI rG'GwC_Cy
Cuts at: 0 155 434 2274
Size: 155 279 1840
Fragments arranged by size:
1840 279 155
PstI C_TGCA'G
Cuts at: 0 666 810 863 984 1775 2274
Size: 666 144 53 121 791 499
Fragments arranged by size:
791 666 499 144 121 53
PvuII CAG'CTG
Cuts at: 0 661 667 811 870 1017 1755 2274
Size: 661 6 144 59 147 738 519
Fragments arranged by size:
738 661 519 147 144 59 6
SacI G_AGCT'C
Cuts at: 0 1900 2274
Size: 1900 374
SalI G'TCGA_C
Cuts at: 0 1705 2274
Size: 1705 569
SbfI CC_TGCA'GG
Cuts at: 0 984 2274
Size: 984 1290
ScaI AGT'ACT
Cuts at: 0 1591 2274
Size: 1591 683
SexAI A'CCwGG_T
Cuts at: 0 172 2274
Size: 172 2102
SfcI C'TryA_G
Cuts at: 0 369 662 806 859 980 1668 1771 2274
Size: 369 293 144 53 121 688 103 503
Fragments arranged by size:
688 503 369 293 144 121 103 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 490 2274
Size: 490 1784
SfoI GGC'GCC
Cuts at: 0 1818 2274
Size: 1818 456
SmaI CCC'GGG
Cuts at: 0 538 888 2274
Size: 538 350 1386
Fragments arranged by size:
1386 538 350
SmlI C'TyrA_G
Cuts at: 0 691 1738 2053 2143 2274
Size: 691 1047 315 90 131
Fragments arranged by size:
1047 691 315 131 90
SphI G_CATG'C
Cuts at: 0 1368 2274
Size: 1368 906
SspI AAT'ATT
Cuts at: 0 2070 2274
Size: 2070 204
StuI AGG'CCT
Cuts at: 0 493 2238 2274
Size: 493 1745 36
Fragments arranged by size:
1745 493 36
StyI C'CwwG_G
Cuts at: 0 151 1322 1470 1601 1811 1946 2274
Size: 151 1171 148 131 210 135 328
Fragments arranged by size:
1171 328 210 151 148 135 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 262 1027 2011 2250 2274
Size: 39 223 765 984 239 24
Fragments arranged by size:
984 765 239 223 39 24
TatI w'GTAC_w
Cuts at: 0 1589 1646 2105 2274
Size: 1589 57 459 169
Fragments arranged by size:
1589 459 169 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 548 580 2032 2064 2274
Size: 548 32 1452 32 210
Fragments arranged by size:
1452 548 210 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 787 1329 2146 2274
Size: 787 542 817 128
Fragments arranged by size:
817 787 542 128
XmaI C'CCGG_G
Cuts at: 0 536 886 2274
Size: 536 350 1388
Fragments arranged by size:
1388 536 350
XmnI GAAnn'nnTTC
Cuts at: 0 1093 1624 2071 2274
Size: 1093 531 447 203
Fragments arranged by size:
1093 531 447 203
Enzymes that do cut:
AarI AccI AclI AcuI AlfI AloI AlwNI AseI
AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NsiI NspI PasI PflMI PfoI PpuMI PstI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI ApoI AscI AsiSI BamHI BcgI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI BspHI
BsrFI BssHII BstBI BstZ17I ClaI DraI DrdI EcoRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SgfI SgrAI
SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI