Restriction map for
pFN21AE6696
(Linear) (Six-base) MAPSORT of: KIEE6696.seq Check: 1373 from: 1 to: 1200
KIEE6696.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 848 1200
Size: 848 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 727 1200
Size: 146 581 473
Fragments arranged by size:
581 473 146
AfeI AGC'GCT
Cuts at: 0 1144 1200
Size: 1144 56
AflIII A'CryG_T
Cuts at: 0 59 919 1200
Size: 59 860 281
Fragments arranged by size:
860 281 59
AleI CACnn'nnGTG
Cuts at: 0 1106 1200
Size: 1106 94
AlwNI CAG_nnn'CTG
Cuts at: 0 124 741 1200
Size: 124 617 459
Fragments arranged by size:
617 459 124
ApaI G_GGCC'C
Cuts at: 0 74 1200
Size: 74 1126
ApoI r'AATT_y
Cuts at: 0 949 1200
Size: 949 251
AvaI C'yCGr_G
Cuts at: 0 67 110 637 1082 1200
Size: 67 43 527 445 118
Fragments arranged by size:
527 445 118 67 43
AvrII C'CTAG_G
Cuts at: 0 267 1200
Size: 267 933
BanI G'GyrC_C
Cuts at: 0 791 1078 1200
Size: 791 287 122
Fragments arranged by size:
791 287 122
BanII G_rGCy'C
Cuts at: 0 74 983 1188 1200
Size: 74 909 205 12
Fragments arranged by size:
909 205 74 12
BbeI G_GCGC'C
Cuts at: 0 795 1200
Size: 795 405
BbsI GAAGACnn'nnnn_
Cuts at: 0 442 940 1200
Size: 442 498 260
Fragments arranged by size:
498 442 260
BbvCI CC'TCA_GC
Cuts at: 0 685 1194 1200
Size: 685 509 6
Fragments arranged by size:
685 509 6
BglI GCCn_nnn'nGGC
Cuts at: 0 14 78 415 832 1200
Size: 14 64 337 417 368
Fragments arranged by size:
417 368 337 64 14
BglII A'GATC_T
Cuts at: 0 92 1200
Size: 92 1108
Bme1580I G_kGCm'C
Cuts at: 0 67 74 590 1083 1200
Size: 67 7 516 493 117
Fragments arranged by size:
516 493 117 67 7
BmgBI CAC'GTC
Cuts at: 0 920 1200
Size: 920 280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 425 766 1125 1159 1200
Size: 298 127 341 359 34 41
Fragments arranged by size:
359 341 298 127 41 34
Bpu10I CC'TnA_GC
Cuts at: 0 523 685 1194 1200
Size: 523 162 509 6
Fragments arranged by size:
523 509 162 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 586 1200
Size: 331 255 614
Fragments arranged by size:
614 331 255
BsaHI Gr'CG_yC
Cuts at: 0 658 792 845 1200
Size: 658 134 53 355
Fragments arranged by size:
658 355 134 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 1200
Size: 289 30 881
Fragments arranged by size:
881 289 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 973 1200
Size: 763 210 227
Fragments arranged by size:
763 227 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 301 376 1200
Size: 294 7 75 824
Fragments arranged by size:
824 294 75 7
BsiEI CG_ry'CG
Cuts at: 0 21 300 1181 1200
Size: 21 279 881 19
Fragments arranged by size:
881 279 21 19
BsiHKAI G_wGCw'C
Cuts at: 0 47 524 983 1200
Size: 47 477 459 217
Fragments arranged by size:
477 459 217 47
Bsp1286I G_dGCh'C
Cuts at: 0 47 67 74 524 590 983 1083 1188
Size: 47 20 7 450 66 393 100 105
Cuts at: 1188 1200
Size: 12
Fragments arranged by size:
450 393 105 100 66 47 20 12
7
BsrBI CCG'CTC
Cuts at: 0 143 1200
Size: 143 1057
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 519 741 1200
Size: 399 120 222 459
Fragments arranged by size:
459 399 222 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1005 1200
Size: 1005 195
BstYI r'GATC_y
Cuts at: 0 92 578 1200
Size: 92 486 622
Fragments arranged by size:
622 486 92
Bsu36I CC'TnA_GG
Cuts at: 0 218 331 883 1200
Size: 218 113 552 317
Fragments arranged by size:
552 317 218 113
BtgI C'CryG_G
Cuts at: 0 353 839 1200
Size: 353 486 361
Fragments arranged by size:
486 361 353
DraIII CAC_nnn'GTG
Cuts at: 0 519 1200
Size: 519 681
DrdI GACnn_nn'nnGTC
Cuts at: 0 382 868 1200
Size: 382 486 332
Fragments arranged by size:
486 382 332
EaeI y'GGCC_r
Cuts at: 0 50 756 992 1178 1200
Size: 50 706 236 186 22
Fragments arranged by size:
706 236 186 50 22
EagI C'GGCC_G
Cuts at: 0 1178 1200
Size: 1178 22
EarI CTCTTCn'nnn_
Cuts at: 0 672 1200
Size: 672 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 583 739 1200
Size: 76 507 156 461
Fragments arranged by size:
507 461 156 76
EcoICRI GAG'CTC
Cuts at: 0 981 1200
Size: 981 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 298 425 727 766 1125 1159 1200
Size: 146 152 127 302 39 359 34 41
Fragments arranged by size:
359 302 152 146 127 41 39 34
EcoO109I rG'GnC_Cy
Cuts at: 0 121 153 342 474 1200
Size: 121 32 189 132 726
Fragments arranged by size:
726 189 132 121 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 1200
Size: 411 789
HaeII r_GCGC'y
Cuts at: 0 645 685 795 1146 1200
Size: 645 40 110 351 54
Fragments arranged by size:
645 351 110 54 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 889 921 1200
Size: 289 32 568 32 279
Fragments arranged by size:
568 289 279 32 32
HincII GTy'rAC
Cuts at: 0 849 1200
Size: 849 351
KasI G'GCGC_C
Cuts at: 0 791 1200
Size: 791 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1200
Size: 695 505
MscI TGG'CCA
Cuts at: 0 52 994 1200
Size: 52 942 206
Fragments arranged by size:
942 206 52
MslI CAynn'nnrTG
Cuts at: 0 58 1106 1200
Size: 58 1048 94
Fragments arranged by size:
1048 94 58
MspA1I CmG'CkG
Cuts at: 0 488 654 679 1200
Size: 488 166 25 521
Fragments arranged by size:
521 488 166 25
NarI GG'CG_CC
Cuts at: 0 792 1200
Size: 792 408
NcoI C'CATG_G
Cuts at: 0 353 839 1200
Size: 353 486 361
Fragments arranged by size:
486 361 353
PasI CC'CwG_GG
Cuts at: 0 1068 1200
Size: 1068 132
PfoI T'CCnGG_A
Cuts at: 0 38 1200
Size: 38 1162
PpuMI rG'GwC_Cy
Cuts at: 0 121 474 1200
Size: 121 353 726
Fragments arranged by size:
726 353 121
PspOMI G'GGCC_C
Cuts at: 0 70 1200
Size: 70 1130
PspXI vC'TCGA_Gb
Cuts at: 0 637 1200
Size: 637 563
PstI C_TGCA'G
Cuts at: 0 195 1121 1200
Size: 195 926 79
Fragments arranged by size:
926 195 79
PvuI CG_AT'CG
Cuts at: 0 21 1200
Size: 21 1179
SacI G_AGCT'C
Cuts at: 0 983 1200
Size: 983 217
SfcI C'TryA_G
Cuts at: 0 191 321 780 1117 1200
Size: 191 130 459 337 83
Fragments arranged by size:
459 337 191 130 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 78 1200
Size: 78 1122
SfoI GGC'GCC
Cuts at: 0 793 1200
Size: 793 407
SmaI CCC'GGG
Cuts at: 0 69 112 1200
Size: 69 43 1088
Fragments arranged by size:
1088 69 43
SmlI C'TyrA_G
Cuts at: 0 346 601 637 1200
Size: 346 255 36 563
Fragments arranged by size:
563 346 255 36
StyI C'CwwG_G
Cuts at: 0 201 267 338 353 839 1200
Size: 201 66 71 15 486 361
Fragments arranged by size:
486 361 201 71 66 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 441 473 1200
Size: 441 32 727
Fragments arranged by size:
727 441 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 1200
Size: 1002 198
XhoI C'TCGA_G
Cuts at: 0 637 1200
Size: 637 563
XmaI C'CCGG_G
Cuts at: 0 67 110 1200
Size: 67 43 1090
Fragments arranged by size:
1090 67 43
ZraI GAC'GTC
Cuts at: 0 846 1200
Size: 846 354
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BstAPI BstXI
BstYI Bsu36I BtgI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI PasI
PfoI PpuMI PspOMI PspXI PstI PvuI SacI SfcI
SfiI SfoI SmaI SmlI StyI TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XmnI