Restriction map for
pFN21AE6695
(Linear) (Six-base) MAPSORT of: KIEE6695.seq Check: 9526 from: 1 to: 1005
KIEE6695.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AflIII A'CryG_T
Cuts at: 0 959 1005
Size: 959 46
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 188 1005
Size: 188 817
AlwNI CAG_nnn'CTG
Cuts at: 0 182 422 504 1005
Size: 182 240 82 501
Fragments arranged by size:
501 240 182 82
ApaI G_GGCC'C
Cuts at: 0 447 1005
Size: 447 558
ApoI r'AATT_y
Cuts at: 0 378 1005
Size: 378 627
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 262 295 1005
Size: 262 33 710
Fragments arranged by size:
710 262 33
BamHI G'GATC_C
Cuts at: 0 878 1005
Size: 878 127
BanI G'GyrC_C
Cuts at: 0 61 406 1005
Size: 61 345 599
Fragments arranged by size:
599 345 61
BanII G_rGCy'C
Cuts at: 0 447 1005
Size: 447 558
BbsI GAAGACnn'nnnn_
Cuts at: 0 611 1005
Size: 611 394
BlpI GC'TnA_GC
Cuts at: 0 306 1005
Size: 306 699
Bme1580I G_kGCm'C
Cuts at: 0 447 1005
Size: 447 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 449 909 1005
Size: 449 460 96
Fragments arranged by size:
460 449 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 469 786 809 1005
Size: 204 265 317 23 196
Fragments arranged by size:
317 265 204 196 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 556 1005
Size: 556 449
BsaWI w'CCGG_w
Cuts at: 0 472 1005
Size: 472 533
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 1005
Size: 38 30 937
Fragments arranged by size:
937 38 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1005
Size: 160 845
BseYI C'CCAG_C
Cuts at: 0 556 1005
Size: 556 449
Bsp1286I G_dGCh'C
Cuts at: 0 447 1005
Size: 447 558
BspHI T'CATG_A
Cuts at: 0 776 1005
Size: 776 229
BsrGI T'GTAC_A
Cuts at: 0 581 1005
Size: 581 424
BstAPI GCAn_nnn'nTGC
Cuts at: 0 850 1005
Size: 850 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 999 1005
Size: 208 791 6
Fragments arranged by size:
791 208 6
BstYI r'GATC_y
Cuts at: 0 878 1005
Size: 878 127
BtgI C'CryG_G
Cuts at: 0 356 1005
Size: 356 649
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 727 1005
Size: 450 277 278
Fragments arranged by size:
450 278 277
DraI TTT'AAA
Cuts at: 0 225 1005
Size: 225 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 381 508 1005
Size: 381 127 497
Fragments arranged by size:
497 381 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 469 786 809 1005
Size: 204 265 317 23 196
Fragments arranged by size:
317 265 204 196 23
EcoO109I rG'GnC_Cy
Cuts at: 0 32 443 444 910 1005
Size: 32 411 1 466 95
Fragments arranged by size:
466 411 95 32 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 1005
Size: 117 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 1005
Size: 38 32 935
Fragments arranged by size:
935 38 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 1005
Size: 672 333
MslI CAynn'nnrTG
Cuts at: 0 206 1005
Size: 206 799
MspA1I CmG'CkG
Cuts at: 0 18 504 1005
Size: 18 486 501
Fragments arranged by size:
501 486 18
NspI r_CATG'y
Cuts at: 0 963 1005
Size: 963 42
PciI A'CATG_T
Cuts at: 0 959 1005
Size: 959 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 182 1005
Size: 182 823
PpuMI rG'GwC_Cy
Cuts at: 0 910 1005
Size: 910 95
PspOMI G'GGCC_C
Cuts at: 0 443 1005
Size: 443 562
PstI C_TGCA'G
Cuts at: 0 167 1005
Size: 167 838
PvuII CAG'CTG
Cuts at: 0 504 1005
Size: 504 501
SanDI GG'GwC_CC
Cuts at: 0 910 1005
Size: 910 95
ScaI AGT'ACT
Cuts at: 0 807 848 1005
Size: 807 41 157
Fragments arranged by size:
807 157 41
SfcI C'TryA_G
Cuts at: 0 135 163 1005
Size: 135 28 842
Fragments arranged by size:
842 135 28
StyI C'CwwG_G
Cuts at: 0 488 543 1005
Size: 488 55 462
Fragments arranged by size:
488 462 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 1005
Size: 589 416
TatI w'GTAC_w
Cuts at: 0 581 805 846 1005
Size: 581 224 41 159
Fragments arranged by size:
581 224 159 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 585 617 1005
Size: 585 32 388
Fragments arranged by size:
585 388 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 628 1005
Size: 444 184 377
Fragments arranged by size:
444 377 184
Enzymes that do cut:
AflIII AlfI AlwNI ApaI ApoI BaeI BamHI BanI
BanII BbsI BlpI Bme1580I BmrI BpmI BsaI BsaWI
BsaXI BseRI BseYI Bsp1286I BspHI BsrGI BstAPI BstXI
BstYI BtgI BtgZI DraI EciI Eco57MI EcoO109I FalI
Hin4I MmeI MslI MspA1I NspI PciI PflMI PpuMI
PspOMI PstI PvuII SanDI ScaI SfcI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI
EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI