Restriction map for
pFN21AE6694
(Linear) (Six-base) MAPSORT of: KIEE6694.seq Check: 8320 from: 1 to: 1194
KIEE6694.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 1194
Size: 360 834
AfeI AGC'GCT
Cuts at: 0 542 1194
Size: 542 652
AflIII A'CryG_T
Cuts at: 0 1115 1194
Size: 1115 79
AhdI GACnn_n'nnGTC
Cuts at: 0 507 1194
Size: 507 687
ApaI G_GGCC'C
Cuts at: 0 91 264 435 1022 1194
Size: 91 173 171 587 172
Fragments arranged by size:
587 173 172 171 91
ApaLI G'TGCA_C
Cuts at: 0 315 1194
Size: 315 879
AvaI C'yCGr_G
Cuts at: 0 65 79 126 280 337 483 628 1124
Size: 65 14 47 154 57 146 145 496
Cuts at: 1124 1185 1194
Size: 61 9
Fragments arranged by size:
496 154 146 145 65 61 57 47
14 9
BanI G'GyrC_C
Cuts at: 0 202 679 778 840 1057 1086 1194
Size: 202 477 99 62 217 29 108
Fragments arranged by size:
477 217 202 108 99 62 29
BanII G_rGCy'C
Cuts at: 0 91 264 344 435 1022 1072 1194
Size: 91 173 80 91 587 50 122
Fragments arranged by size:
587 173 122 91 91 80 50
BbeI G_GCGC'C
Cuts at: 0 206 683 844 1194
Size: 206 477 161 350
Fragments arranged by size:
477 350 206 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 1194
Size: 201 993
BglI GCCn_nnn'nGGC
Cuts at: 0 28 40 67 149 268 430 776 1194
Size: 28 12 27 82 119 162 346 418
Fragments arranged by size:
418 346 162 119 82 28 27 12
BlpI GC'TnA_GC
Cuts at: 0 395 412 712 727 1194
Size: 395 17 300 15 467
Fragments arranged by size:
467 395 300 17 15
Bme1580I G_kGCm'C
Cuts at: 0 91 264 319 435 641 1022 1194
Size: 91 173 55 116 206 381 172
Fragments arranged by size:
381 206 173 172 116 91 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 1194
Size: 318 876
Bpu10I CC'TnA_GC
Cuts at: 0 115 1194
Size: 115 1079
BsaAI yAC'GTr
Cuts at: 0 252 1116 1194
Size: 252 864 78
Fragments arranged by size:
864 252 78
BsaHI Gr'CG_yC
Cuts at: 0 203 369 680 841 986 1194
Size: 203 166 311 161 145 208
Fragments arranged by size:
311 208 203 166 161 145
BsaWI w'CCGG_w
Cuts at: 0 1072 1194
Size: 1072 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1067 1097 1194
Size: 1067 30 97
Fragments arranged by size:
1067 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 598 607 610 1194
Size: 535 63 9 3 584
Fragments arranged by size:
584 535 63 9 3
BseYI C'CCAG_C
Cuts at: 0 543 561 1194
Size: 543 18 633
Fragments arranged by size:
633 543 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 274 1194
Size: 232 42 920
Fragments arranged by size:
920 232 42
BsiEI CG_ry'CG
Cuts at: 0 35 200 272 1194
Size: 35 165 72 922
Fragments arranged by size:
922 165 72 35
BsiHKAI G_wGCw'C
Cuts at: 0 319 728 1194
Size: 319 409 466
Fragments arranged by size:
466 409 319
Bsp1286I G_dGCh'C
Cuts at: 0 91 264 319 344 435 641 728 1022
Size: 91 173 55 25 91 206 87 294
Cuts at: 1022 1072 1194
Size: 50 122
Fragments arranged by size:
294 206 173 122 91 91 87 55
50 25
BspEI T'CCGG_A
Cuts at: 0 1072 1194
Size: 1072 122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 360 1194
Size: 248 112 834
Fragments arranged by size:
834 248 112
BsrBI CCG'CTC
Cuts at: 0 297 846 1194
Size: 297 549 348
Fragments arranged by size:
549 348 297
BsrDI GCAATG_nn'
Cuts at: 0 652 1194
Size: 652 542
BsrFI r'CCGG_y
Cuts at: 0 199 739 859 1194
Size: 199 540 120 335
Fragments arranged by size:
540 335 199 120
BssHII G'CGCG_C
Cuts at: 0 442 1194
Size: 442 752
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1143 1194
Size: 1143 51
BtgI C'CryG_G
Cuts at: 0 91 144 194 272 363 611 812 991
Size: 91 53 50 78 91 248 201 179
Cuts at: 991 1034 1194
Size: 43 160
Fragments arranged by size:
248 201 179 160 91 91 78 53
50 43
BtsI GCAGTG_nn'
Cuts at: 0 16 262 745 1046 1194
Size: 16 246 483 301 148
Fragments arranged by size:
483 301 246 148 16
EaeI y'GGCC_r
Cuts at: 0 32 197 269 737 959 1194
Size: 32 165 72 468 222 235
Fragments arranged by size:
468 235 222 165 72 32
EagI C'GGCC_G
Cuts at: 0 32 197 269 1194
Size: 32 165 72 925
Fragments arranged by size:
925 165 72 32
EarI CTCTTCn'nnn_
Cuts at: 0 454 1194
Size: 454 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 541 1194
Size: 541 653
EcoO109I rG'GnC_Cy
Cuts at: 0 261 277 1019 1140 1194
Size: 261 16 742 121 54
Fragments arranged by size:
742 261 121 54 16
FseI GG_CCGG'CC
Cuts at: 0 743 1194
Size: 743 451
HaeII r_GCGC'y
Cuts at: 0 62 206 480 544 683 823 832 844
Size: 62 144 274 64 139 140 9 12
Cuts at: 844 1194
Size: 350
Fragments arranged by size:
350 274 144 140 139 64 62 12
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 1194
Size: 493 32 669
Fragments arranged by size:
669 493 32
KasI G'GCGC_C
Cuts at: 0 202 679 840 1194
Size: 202 477 161 354
Fragments arranged by size:
477 354 202 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 832 1194
Size: 491 341 362
Fragments arranged by size:
491 362 341
MslI CAynn'nnrTG
Cuts at: 0 616 1194
Size: 616 578
MspA1I CmG'CkG
Cuts at: 0 104 196 274 365 466 814 952 1036
Size: 104 92 78 91 101 348 138 84
Cuts at: 1036 1194
Size: 158
Fragments arranged by size:
348 158 138 104 101 92 91 84
78
NaeI GCC'GGC
Cuts at: 0 201 741 861 1194
Size: 201 540 120 333
Fragments arranged by size:
540 333 201 120
NarI GG'CG_CC
Cuts at: 0 203 680 841 1194
Size: 203 477 161 353
Fragments arranged by size:
477 353 203 161
NcoI C'CATG_G
Cuts at: 0 611 1194
Size: 611 583
NgoMIV G'CCGG_C
Cuts at: 0 199 739 859 1194
Size: 199 540 120 335
Fragments arranged by size:
540 335 199 120
PasI CC'CwG_GG
Cuts at: 0 307 1194
Size: 307 887
PmlI CAC'GTG
Cuts at: 0 252 1116 1194
Size: 252 864 78
Fragments arranged by size:
864 252 78
PpuMI rG'GwC_Cy
Cuts at: 0 277 1140 1194
Size: 277 863 54
Fragments arranged by size:
863 277 54
PspOMI G'GGCC_C
Cuts at: 0 87 260 431 1018 1194
Size: 87 173 171 587 176
Fragments arranged by size:
587 176 173 171 87
PspXI vC'TCGA_Gb
Cuts at: 0 337 1194
Size: 337 857
PstI C_TGCA'G
Cuts at: 0 103 380 1194
Size: 103 277 814
Fragments arranged by size:
814 277 103
SacII CC_GC'GG
Cuts at: 0 197 275 366 815 1037 1194
Size: 197 78 91 449 222 157
Fragments arranged by size:
449 222 197 157 91 78
SanDI GG'GwC_CC
Cuts at: 0 277 1194
Size: 277 917
SfcI C'TryA_G
Cuts at: 0 99 376 1194
Size: 99 277 818
Fragments arranged by size:
818 277 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 268 430 1194
Size: 268 162 764
Fragments arranged by size:
764 268 162
SfoI GGC'GCC
Cuts at: 0 204 681 842 1194
Size: 204 477 161 352
Fragments arranged by size:
477 352 204 161
SmaI CCC'GGG
Cuts at: 0 67 128 282 1187 1194
Size: 67 61 154 905 7
Fragments arranged by size:
905 154 67 61 7
SmlI C'TyrA_G
Cuts at: 0 337 483 1194
Size: 337 146 711
Fragments arranged by size:
711 337 146
SrfI GCCC'GGGC
Cuts at: 0 128 1194
Size: 128 1066
StyI C'CwwG_G
Cuts at: 0 611 641 1194
Size: 611 30 553
Fragments arranged by size:
611 553 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 858 1194
Size: 858 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1067 1099 1194
Size: 1067 32 95
Fragments arranged by size:
1067 95 32
XhoI C'TCGA_G
Cuts at: 0 337 483 1194
Size: 337 146 711
Fragments arranged by size:
711 337 146
XmaI C'CCGG_G
Cuts at: 0 65 126 280 1185 1194
Size: 65 61 154 905 9
Fragments arranged by size:
905 154 65 61 9
Enzymes that do cut:
AarI AfeI AflIII AhdI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BmrI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BstXI BtgI BtsI EaeI EagI EarI EciI EcoO109I
FseI HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PmlI PpuMI PspOMI PspXI
PstI SacII SanDI SfcI SfiI SfoI SmaI SmlI
SrfI StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AleI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI
BsmI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TatI Tth111I XbaI XcmI XmnI ZraI