(Linear) (Six-base) MAPSORT of: KIEE6684.seq  Check: 9497  from: 1  to: 1020



KIEE6684.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AclI AA'CG_TT

Cuts at:      0     338    1020 
   Size:        338     682
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1020 
   Size:         94     926
 
AleI CACnn'nnGTG

Cuts at:      0     146     919    1020 
   Size:        146     773     101

  Fragments arranged by size: 

                 773     146     101
 
AlwNI CAG_nnn'CTG

Cuts at:      0     123     994    1020 
   Size:        123     871      26

  Fragments arranged by size: 

                 871     123      26
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     555     588    1020 
   Size:        555      33     432

  Fragments arranged by size: 

                 555     432      33
 
BanII G_rGCy'C

Cuts at:      0      14    1020 
   Size:         14    1006
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     383     722    1020 
   Size:        383     339     298

  Fragments arranged by size: 

                 383     339     298
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     541     575    1020 
   Size:        541      34     445

  Fragments arranged by size: 

                 541     445      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     819    1020 
   Size:        819     201
 
BfrBI ATG'CAT

Cuts at:      0     454     620    1020 
   Size:        454     166     400

  Fragments arranged by size: 

                 454     400     166
 
BmgBI CAC'GTC

Cuts at:      0     148    1020 
   Size:        148     872
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1004    1020 
   Size:       1004      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     136    1020 
   Size:        136     884
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     160     204    1020 
   Size:        160      44     816

  Fragments arranged by size: 

                 816     160      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     772     802    1020 
   Size:        772      30     218

  Fragments arranged by size: 

                 772     218      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     547    1020 
   Size:        547     473
 
BseYI C'CCAG_C

Cuts at:      0     306    1002    1020 
   Size:        306     696      18

  Fragments arranged by size: 

                 696     306      18
 
BsiHKAI G_wGCw'C

Cuts at:      0      14    1020 
   Size:         14    1006
 
BsmI GAATG_Cn'

Cuts at:      0     622    1020 
   Size:        622     398
 
Bsp1286I G_dGCh'C

Cuts at:      0      14    1020 
   Size:         14    1006
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     148    1020 
   Size:        148     872
 
Bsu36I CC'TnA_GG

Cuts at:      0     530     739    1020 
   Size:        530     209     281

  Fragments arranged by size: 

                 530     281     209
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     176     211    1020 
   Size:        176      35     809

  Fragments arranged by size: 

                 809     176      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     505    1020 
   Size:        505     515
 
EaeI y'GGCC_r

Cuts at:      0     464    1020 
   Size:        464     556
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     352    1020 
   Size:         94     258     668

  Fragments arranged by size: 

                 668     258      94
 
EcoICRI GAG'CTC

Cuts at:      0      12    1020 
   Size:         12    1008
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1020 
   Size:         94     926
 
EcoO109I rG'GnC_Cy

Cuts at:      0     670     963    1020 
   Size:        670     293      57

  Fragments arranged by size: 

                 670     293      57
 
FspI TGC'GCA

Cuts at:      0     551    1020 
   Size:        551     469
 
HaeII r_GCGC'y

Cuts at:      0     810    1020 
   Size:        810     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     496     528    1020 
   Size:        496      32     492

  Fragments arranged by size: 

                 496     492      32
 
HindIII A'AGCT_T

Cuts at:      0     837    1020 
   Size:        837     183
 
MscI TGG'CCA

Cuts at:      0     466    1020 
   Size:        466     554
 
MslI CAynn'nnrTG

Cuts at:      0     146     283     493     919    1020 
   Size:        146     137     210     426     101

  Fragments arranged by size: 

                 426     210     146     137     101
 
MspA1I CmG'CkG

Cuts at:      0      83    1020 
   Size:         83     937
 
NsiI A_TGCA'T

Cuts at:      0     456     622    1020 
   Size:        456     166     398

  Fragments arranged by size: 

                 456     398     166
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     123     473    1020 
   Size:        123     350     547

  Fragments arranged by size: 

                 547     350     123
 
PfoI T'CCnGG_A

Cuts at:      0     722    1020 
   Size:        722     298
 
PpuMI rG'GwC_Cy

Cuts at:      0     963    1020 
   Size:        963      57
 
PvuII CAG'CTG

Cuts at:      0      83    1020 
   Size:         83     937
 
SacI G_AGCT'C

Cuts at:      0      14    1020 
   Size:         14    1006
 
SspI AAT'ATT

Cuts at:      0     581    1020 
   Size:        581     439
 
StyI C'CwwG_G

Cuts at:      0     666    1020 
   Size:        666     354
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     468     500    1020 
   Size:        468      32     520

  Fragments arranged by size: 

                 520     468      32
 
XbaI T'CTAG_A

Cuts at:      0     162    1020 
   Size:        162     858
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     392    1020 
   Size:        392     628
 

 Enzymes that do cut:

     AclI     AcuI     AleI    AlwNI     BaeI    BanII     BbsI     BcgI
    BciVI    BfrBI    BmgBI     BmrI   Bpu10I     BsaI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BstXI   Bsu36I    CspCI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
  HindIII     MscI     MslI   MspA1I     NsiI    PflMI     PfoI    PpuMI
    PvuII     SacI     SspI     StyI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BclI
     BglI    BglII     BlpI Bme1580I     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
  Tth111I     XhoI     XmaI     XmnI     ZraI