Restriction map for
pFN21AE6681
(Linear) (Six-base) MAPSORT of: KIEE6681.seq Check: 1292 from: 1 to: 1020
KIEE6681.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 798 1020
Size: 798 222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 580 902 991 1020
Size: 443 137 322 89 29
Fragments arranged by size:
443 322 137 89 29
ApaI G_GGCC'C
Cuts at: 0 301 1020
Size: 301 719
ApoI r'AATT_y
Cuts at: 0 448 492 1020
Size: 448 44 528
Fragments arranged by size:
528 448 44
AvaI C'yCGr_G
Cuts at: 0 377 1020
Size: 377 643
BanII G_rGCy'C
Cuts at: 0 301 1020
Size: 301 719
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 359 620 1020
Size: 290 69 261 400
Fragments arranged by size:
400 290 261 69
BbvCI CC'TCA_GC
Cuts at: 0 55 1020
Size: 55 965
BglII A'GATC_T
Cuts at: 0 723 1020
Size: 723 297
Bme1580I G_kGCm'C
Cuts at: 0 301 1020
Size: 301 719
Bpu10I CC'TnA_GC
Cuts at: 0 55 1020
Size: 55 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1020
Size: 557 463
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 586 715 1020
Size: 355 231 129 305
Fragments arranged by size:
355 305 231 129
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 623 1020
Size: 623 397
BseYI C'CCAG_C
Cuts at: 0 754 1020
Size: 754 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1020
Size: 227 793
BsiHKAI G_wGCw'C
Cuts at: 0 215 1020
Size: 215 805
BsmBI CGTCTCn'nnnn_
Cuts at: 0 74 1020
Size: 74 946
Bsp1286I G_dGCh'C
Cuts at: 0 215 301 1020
Size: 215 86 719
Fragments arranged by size:
719 215 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 798 1020
Size: 798 222
BsrDI GCAATG_nn'
Cuts at: 0 524 1020
Size: 524 496
BsrGI T'GTAC_A
Cuts at: 0 731 1020
Size: 731 289
BssHII G'CGCG_C
Cuts at: 0 28 1020
Size: 28 992
BssSI C'ACGA_G
Cuts at: 0 773 1020
Size: 773 247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 1020
Size: 342 678
BstYI r'GATC_y
Cuts at: 0 723 1020
Size: 723 297
BtgI C'CryG_G
Cuts at: 0 788 833 1020
Size: 788 45 187
Fragments arranged by size:
788 187 45
BtsI GCAGTG_nn'
Cuts at: 0 146 215 548 1020
Size: 146 69 333 472
Fragments arranged by size:
472 333 146 69
DraI TTT'AAA
Cuts at: 0 903 1020
Size: 903 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 361 1020
Size: 361 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 580 902 991 1020
Size: 443 137 322 89 29
Fragments arranged by size:
443 322 137 89 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 575 1020
Size: 575 445
EcoO109I rG'GnC_Cy
Cuts at: 0 298 381 929 1020
Size: 298 83 548 91
Fragments arranged by size:
548 298 91 83
EcoRV GAT'ATC
Cuts at: 0 84 1020
Size: 84 936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 948 1020
Size: 948 72
HaeII r_GCGC'y
Cuts at: 0 7 52 1020
Size: 7 45 968
Fragments arranged by size:
968 45 7
HincII GTy'rAC
Cuts at: 0 324 1020
Size: 324 696
MspA1I CmG'CkG
Cuts at: 0 79 1020
Size: 79 941
NcoI C'CATG_G
Cuts at: 0 788 1020
Size: 788 232
NspI r_CATG'y
Cuts at: 0 963 1020
Size: 963 57
PfoI T'CCnGG_A
Cuts at: 0 839 1020
Size: 839 181
PpuMI rG'GwC_Cy
Cuts at: 0 381 1020
Size: 381 639
PspOMI G'GGCC_C
Cuts at: 0 297 1020
Size: 297 723
PvuII CAG'CTG
Cuts at: 0 79 1020
Size: 79 941
SmaI CCC'GGG
Cuts at: 0 379 1020
Size: 379 641
SmlI C'TyrA_G
Cuts at: 0 572 1020
Size: 572 448
StyI C'CwwG_G
Cuts at: 0 788 942 1020
Size: 788 154 78
Fragments arranged by size:
788 154 78
TatI w'GTAC_w
Cuts at: 0 122 731 1020
Size: 122 609 289
Fragments arranged by size:
609 289 122
XbaI T'CTAG_A
Cuts at: 0 588 1020
Size: 588 432
XmaI C'CCGG_G
Cuts at: 0 377 1020
Size: 377 643
XmnI GAAnn'nnTTC
Cuts at: 0 617 1020
Size: 617 403
Enzymes that do cut:
AarI AcuI ApaI ApoI AvaI BanII BbsI BbvCI
BglII Bme1580I Bpu10I BpuEI BsaI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BssHII BssSI
BstXI BstYI BtgI BtsI DraI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII HincII MspA1I NcoI NspI
PfoI PpuMI PspOMI PvuII SmaI SmlI StyI TatI
XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI EcoRI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XcmI XhoI
ZraI