(Linear) (Six-base) MAPSORT of: KIEE6672.seq  Check: 7199  from: 1  to: 921



KIEE6672.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     921 
   Size:        108     813
 
AflII C'TTAA_G

Cuts at:      0     594     921 
   Size:        594     327
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      62     921 
   Size:         62     859
 
AlwNI CAG_nnn'CTG

Cuts at:      0     159     921 
   Size:        159     762
 
ApoI r'AATT_y

Cuts at:      0     219     417     470     921 
   Size:        219     198      53     451

  Fragments arranged by size: 

                 451     219     198      53
 
BanI G'GyrC_C

Cuts at:      0     859     921 
   Size:        859      62
 
BbvCI CC'TCA_GC

Cuts at:      0     115     921 
   Size:        115     806
 
BclI T'GATC_A

Cuts at:      0     501     921 
   Size:        501     420
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     801     921 
   Size:        801     120
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     104     921 
   Size:        104     817
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     921 
   Size:        149     772
 
Bpu10I CC'TnA_GC

Cuts at:      0     115     921 
   Size:        115     806
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     921 
   Size:         37     884
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     921 
   Size:         58     863
 
BsmI GAATG_Cn'

Cuts at:      0     408     921 
   Size:        408     513
 
BspHI T'CATG_A

Cuts at:      0     303     870     921 
   Size:        303     567      51

  Fragments arranged by size: 

                 567     303      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     906     921 
   Size:        906      15
 
BsrDI GCAATG_nn'

Cuts at:      0      44     921 
   Size:         44     877
 
BsrFI r'CCGG_y

Cuts at:      0      24     921 
   Size:         24     897
 
BstYI r'GATC_y

Cuts at:      0     156     921 
   Size:        156     765
 
BtsI GCAGTG_nn'

Cuts at:      0     653     921 
   Size:        653     268
 
DraI TTT'AAA

Cuts at:      0     833     921 
   Size:        833      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     149     921 
   Size:        108      41     772

  Fragments arranged by size: 

                 772     108      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     100     358     921 
   Size:        100     258     563

  Fragments arranged by size: 

                 563     258     100
 
EcoRV GAT'ATC

Cuts at:      0     609     921 
   Size:        609     312
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     921 
   Size:         27     894
 
MfeI C'AATT_G

Cuts at:      0     641     921 
   Size:        641     280
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     744     921 
   Size:        744     177
 
MslI CAynn'nnrTG

Cuts at:      0      98     921 
   Size:         98     823
 
NaeI GCC'GGC

Cuts at:      0      26     921 
   Size:         26     895
 
NgoMIV G'CCGG_C

Cuts at:      0      24     921 
   Size:         24     897
 
NspI r_CATG'y

Cuts at:      0     760     883     921 
   Size:        760     123      38

  Fragments arranged by size: 

                 760     123      38
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     767     799     921 
   Size:        767      32     122

  Fragments arranged by size: 

                 767     122      32
 
SmlI C'TyrA_G

Cuts at:      0      16     594     921 
   Size:         16     578     327

  Fragments arranged by size: 

                 578     327      16
 
SphI G_CATG'C

Cuts at:      0     883     921 
   Size:        883      38
 
SspI AAT'ATT

Cuts at:      0     696     921 
   Size:        696     225
 
StuI AGG'CCT

Cuts at:      0     353     921 
   Size:        353     568
 
StyI C'CwwG_G

Cuts at:      0     322     921 
   Size:        322     599
 
SwaI ATTT'AAAT

Cuts at:      0     833     921 
   Size:        833      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      83     921 
   Size:         83     838
 
XbaI T'CTAG_A

Cuts at:      0     477     921 
   Size:        477     444
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210     921 
   Size:        210     711
 

 Enzymes that do cut:

     AcuI    AflII     AlfI    AlwNI     ApoI     BanI    BbvCI     BclI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsgI     BsmI    BspHI
    BspMI    BsrDI    BsrFI    BstYI     BtsI     DraI  Eco57MI    EcoNI
    EcoRV    Hin4I     MfeI     MmeI     MslI     NaeI   NgoMIV     NspI
     PsrI     SmlI     SphI     SspI     StuI     StyI     SwaI    TaqII
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     TatI     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI