Restriction map for
pFN21AE6668
(Linear) (Six-base) MAPSORT of: KIEE6668.seq Check: 1878 from: 1 to: 888
KIEE6668.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 685 888
Size: 685 203
Acc65I G'GTAC_C
Cuts at: 0 460 888
Size: 460 428
AlwNI CAG_nnn'CTG
Cuts at: 0 342 888
Size: 342 546
ApoI r'AATT_y
Cuts at: 0 611 888
Size: 611 277
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 444 477 888
Size: 444 33 411
Fragments arranged by size:
444 411 33
BanI G'GyrC_C
Cuts at: 0 460 888
Size: 460 428
BanII G_rGCy'C
Cuts at: 0 258 888
Size: 258 630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 629 888
Size: 595 34 259
Fragments arranged by size:
595 259 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 305 888
Size: 305 583
BmgBI CAC'GTC
Cuts at: 0 201 888
Size: 201 687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 888
Size: 105 783
BsaAI yAC'GTr
Cuts at: 0 528 888
Size: 528 360
BsaHI Gr'CG_yC
Cuts at: 0 682 888
Size: 682 206
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 888
Size: 115 773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 888
Size: 117 771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 673 888
Size: 673 215
Bsp1286I G_dGCh'C
Cuts at: 0 258 888
Size: 258 630
BspHI T'CATG_A
Cuts at: 0 767 888
Size: 767 121
BsrDI GCAATG_nn'
Cuts at: 0 302 888
Size: 302 586
BstXI CCAn_nnnn'nTGG
Cuts at: 0 274 888
Size: 274 614
BstYI r'GATC_y
Cuts at: 0 92 888
Size: 92 796
BtgI C'CryG_G
Cuts at: 0 307 888
Size: 307 581
EaeI y'GGCC_r
Cuts at: 0 259 888
Size: 259 629
EarI CTCTTCn'nnn_
Cuts at: 0 464 574 580 888
Size: 464 110 6 308
Fragments arranged by size:
464 308 110 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 888
Size: 105 783
EcoNI CCTnn'n_nnAGG
Cuts at: 0 146 888
Size: 146 742
EcoO109I rG'GnC_Cy
Cuts at: 0 53 597 888
Size: 53 544 291
Fragments arranged by size:
544 291 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 888
Size: 209 32 647
Fragments arranged by size:
647 209 32
KpnI G_GTAC'C
Cuts at: 0 464 888
Size: 464 424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 888
Size: 252 636
MspA1I CmG'CkG
Cuts at: 0 339 888
Size: 339 549
NspI r_CATG'y
Cuts at: 0 447 888
Size: 447 441
PfoI T'CCnGG_A
Cuts at: 0 674 888
Size: 674 214
PmlI CAC'GTG
Cuts at: 0 528 888
Size: 528 360
PpuMI rG'GwC_Cy
Cuts at: 0 53 597 888
Size: 53 544 291
Fragments arranged by size:
544 291 53
PstI C_TGCA'G
Cuts at: 0 65 888
Size: 65 823
PvuII CAG'CTG
Cuts at: 0 339 888
Size: 339 549
SanDI GG'GwC_CC
Cuts at: 0 53 597 888
Size: 53 544 291
Fragments arranged by size:
544 291 53
SfcI C'TryA_G
Cuts at: 0 61 725 840 888
Size: 61 664 115 48
Fragments arranged by size:
664 115 61 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 629 661 888
Size: 629 32 227
Fragments arranged by size:
629 227 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 353 405 741 888
Size: 353 52 336 147
Fragments arranged by size:
353 336 147 52
ZraI GAC'GTC
Cuts at: 0 683 888
Size: 683 205
Enzymes that do cut:
AatII Acc65I AlwNI ApoI BaeI BanI BanII BcgI
BciVI BmgBI BpmI BsaAI BsaHI BseRI BsgI BsmBI
Bsp1286I BspHI BsrDI BstXI BstYI BtgI EaeI EarI
Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI MspA1I NspI
PfoI PmlI PpuMI PstI PvuII SanDI SfcI TstI
XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI