(Linear) (Six-base) MAPSORT of: KIEE6667.seq  Check: 4341  from: 1  to: 894



KIEE6667.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     894 
   Size:        578     316
 
ApoI r'AATT_y

Cuts at:      0     190     894 
   Size:        190     704
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     126     159     894 
   Size:        126      33     735

  Fragments arranged by size: 

                 735     126      33
 
BanI G'GyrC_C

Cuts at:      0     866     894 
   Size:        866      28
 
BanII G_rGCy'C

Cuts at:      0     131     546     894 
   Size:        131     415     348

  Fragments arranged by size: 

                 415     348     131
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     431     894 
   Size:        431     463
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     780     814     894 
   Size:        780      34      80

  Fragments arranged by size: 

                 780      80      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     653     894 
   Size:        653     241
 
BclI T'GATC_A

Cuts at:      0     326     894 
   Size:        326     568
 
BmgBI CAC'GTC

Cuts at:      0     621     894 
   Size:        621     273
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     759     894 
   Size:        759     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     560     894 
   Size:        536      24     334

  Fragments arranged by size: 

                 536     334      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     701     894 
   Size:        701     193
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     863     894 
   Size:        863      31
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433     894 
   Size:        433     461
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     894 
   Size:        146     748
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     546     894 
   Size:        131     415     348

  Fragments arranged by size: 

                 415     348     131
 
BtsI GCAGTG_nn'

Cuts at:      0     279     365     894 
   Size:        279      86     529

  Fragments arranged by size: 

                 529     279      86
 
EarI CTCTTCn'nnn_

Cuts at:      0      96     101     894 
   Size:         96       5     793

  Fragments arranged by size: 

                 793      96       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     560     578     894 
   Size:        536      24      18     316

  Fragments arranged by size: 

                 536     316      24      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     405     894 
   Size:        405     489
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     894 
   Size:        249     645
 
HaeII r_GCGC'y

Cuts at:      0     395     894 
   Size:        395     499
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      67      99     550     582     894 
   Size:         67      32     451      32     312

  Fragments arranged by size: 

                 451     312      67      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     743     869     894 
   Size:        743     126      25

  Fragments arranged by size: 

                 743     126      25
 
MslI CAynn'nnrTG

Cuts at:      0     713     793     894 
   Size:        713      80     101

  Fragments arranged by size: 

                 713     101      80
 
NspI r_CATG'y

Cuts at:      0     471     894 
   Size:        471     423
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     894 
   Size:        249     645
 
SmlI C'TyrA_G

Cuts at:      0     878     894 
   Size:        878      16
 
StuI AGG'CCT

Cuts at:      0     821     894 
   Size:        821      73
 
XmnI GAAnn'nnTTC

Cuts at:      0     487     894 
   Size:        487     407
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BseRI     BsgI
 Bsp1286I     BtsI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     MmeI     MslI     NspI    PpuMI     SmlI     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI