Restriction map for
pFN21AE6666
(Linear) (Six-base) MAPSORT of: KIEE6666.seq Check: 9925 from: 1 to: 870
KIEE6666.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 870
Size: 321 549
AatII G_ACGT'C
Cuts at: 0 634 870
Size: 634 236
Acc65I G'GTAC_C
Cuts at: 0 476 870
Size: 476 394
ApoI r'AATT_y
Cuts at: 0 520 727 870
Size: 520 207 143
Fragments arranged by size:
520 207 143
AseI AT'TA_AT
Cuts at: 0 409 870
Size: 409 461
BamHI G'GATC_C
Cuts at: 0 856 870
Size: 856 14
BanI G'GyrC_C
Cuts at: 0 435 476 870
Size: 435 41 394
Fragments arranged by size:
435 394 41
BanII G_rGCy'C
Cuts at: 0 234 870
Size: 234 636
BclI T'GATC_A
Cuts at: 0 168 870
Size: 168 702
BfrBI ATG'CAT
Cuts at: 0 126 870
Size: 126 744
BglII A'GATC_T
Cuts at: 0 750 870
Size: 750 120
Bme1580I G_kGCm'C
Cuts at: 0 842 870
Size: 842 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 237 870
Size: 237 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 272 870
Size: 190 82 598
Fragments arranged by size:
598 190 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 870
Size: 103 767
BsaHI Gr'CG_yC
Cuts at: 0 631 870
Size: 631 239
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 372 870
Size: 372 498
BseYI C'CCAG_C
Cuts at: 0 367 870
Size: 367 503
BsiHKAI G_wGCw'C
Cuts at: 0 89 870
Size: 89 781
BsmBI CGTCTCn'nnnn_
Cuts at: 0 638 870
Size: 638 232
Bsp1286I G_dGCh'C
Cuts at: 0 89 234 842 870
Size: 89 145 608 28
Fragments arranged by size:
608 145 89 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 632 870
Size: 321 311 238
Fragments arranged by size:
321 311 238
BssSI C'ACGA_G
Cuts at: 0 835 870
Size: 835 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 449 870
Size: 449 421
BstEII G'GTnAC_C
Cuts at: 0 333 870
Size: 333 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 870
Size: 147 723
BstYI r'GATC_y
Cuts at: 0 92 750 856 870
Size: 92 658 106 14
Fragments arranged by size:
658 106 92 14
Bsu36I CC'TnA_GG
Cuts at: 0 431 870
Size: 431 439
BtgI C'CryG_G
Cuts at: 0 140 870
Size: 140 730
DraI TTT'AAA
Cuts at: 0 765 870
Size: 765 105
EarI CTCTTCn'nnn_
Cuts at: 0 455 776 870
Size: 455 321 94
Fragments arranged by size:
455 321 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 272 870
Size: 190 82 598
Fragments arranged by size:
598 190 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 541 837 870
Size: 541 296 33
Fragments arranged by size:
541 296 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 592 624 870
Size: 355 32 205 32 246
Fragments arranged by size:
355 246 205 32 32
HincII GTy'rAC
Cuts at: 0 294 870
Size: 294 576
HpaI GTT'AAC
Cuts at: 0 294 870
Size: 294 576
KpnI G_GTAC'C
Cuts at: 0 480 870
Size: 480 390
MfeI C'AATT_G
Cuts at: 0 593 870
Size: 593 277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 870
Size: 661 209
MslI CAynn'nnrTG
Cuts at: 0 310 870
Size: 310 560
NcoI C'CATG_G
Cuts at: 0 140 870
Size: 140 730
NsiI A_TGCA'T
Cuts at: 0 128 870
Size: 128 742
NspI r_CATG'y
Cuts at: 0 126 870
Size: 126 744
PsiI TTA'TAA
Cuts at: 0 652 737 870
Size: 652 85 133
Fragments arranged by size:
652 133 85
PstI C_TGCA'G
Cuts at: 0 332 629 870
Size: 332 297 241
Fragments arranged by size:
332 297 241
SbfI CC_TGCA'GG
Cuts at: 0 332 870
Size: 332 538
SexAI A'CCwGG_T
Cuts at: 0 846 870
Size: 846 24
SfcI C'TryA_G
Cuts at: 0 328 625 720 870
Size: 328 297 95 150
Fragments arranged by size:
328 297 150 95
SmlI C'TyrA_G
Cuts at: 0 82 870
Size: 82 788
StyI C'CwwG_G
Cuts at: 0 140 821 870
Size: 140 681 49
Fragments arranged by size:
681 140 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 185 870
Size: 185 685
TatI w'GTAC_w
Cuts at: 0 224 870
Size: 224 646
XbaI T'CTAG_A
Cuts at: 0 402 870
Size: 402 468
ZraI GAC'GTC
Cuts at: 0 632 870
Size: 632 238
Enzymes that do cut:
AarI AatII Acc65I ApoI AseI BamHI BanI BanII
BclI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaHI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI DraI EarI Eco57MI
FalI Hin4I HincII HpaI KpnI MfeI MmeI MslI
NcoI NsiI NspI PsiI PstI SbfI SexAI SfcI
SmlI StyI TaqII TatI XbaI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HindIII KasI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XcmI XhoI XmaI XmnI