Restriction map for
pFN21AE6659
(Linear) (Six-base) MAPSORT of: KIEE6659.seq Check: 9000 from: 1 to: 870
KIEE6659.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 870
Size: 321 549
AflII C'TTAA_G
Cuts at: 0 580 870
Size: 580 290
AlwNI CAG_nnn'CTG
Cuts at: 0 402 870
Size: 402 468
ApoI r'AATT_y
Cuts at: 0 520 870
Size: 520 350
BanI G'GyrC_C
Cuts at: 0 435 870
Size: 435 435
BanII G_rGCy'C
Cuts at: 0 234 870
Size: 234 636
BbsI GAAGACnn'nnnn_
Cuts at: 0 438 551 870
Size: 438 113 319
Fragments arranged by size:
438 319 113
BclI T'GATC_A
Cuts at: 0 168 870
Size: 168 702
BglII A'GATC_T
Cuts at: 0 750 870
Size: 750 120
Bme1580I G_kGCm'C
Cuts at: 0 842 870
Size: 842 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 237 870
Size: 237 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 272 424 870
Size: 190 82 152 446
Fragments arranged by size:
446 190 152 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 870
Size: 247 623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 260 290 870
Size: 260 30 580
Fragments arranged by size:
580 260 30
BseYI C'CCAG_C
Cuts at: 0 367 870
Size: 367 503
Bsp1286I G_dGCh'C
Cuts at: 0 234 842 870
Size: 234 608 28
Fragments arranged by size:
608 234 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 632 870
Size: 321 311 238
Fragments arranged by size:
321 311 238
BstEII G'GTnAC_C
Cuts at: 0 333 870
Size: 333 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 870
Size: 264 606
BstYI r'GATC_y
Cuts at: 0 92 750 870
Size: 92 658 120
Fragments arranged by size:
658 120 92
Bsu36I CC'TnA_GG
Cuts at: 0 431 870
Size: 431 439
BtgI C'CryG_G
Cuts at: 0 140 870
Size: 140 730
EarI CTCTTCn'nnn_
Cuts at: 0 455 870
Size: 455 415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 272 424 870
Size: 190 82 152 446
Fragments arranged by size:
446 190 152 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 34 541 870
Size: 34 507 329
Fragments arranged by size:
507 329 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 188 203 220 592 624 870
Size: 171 17 15 17 372 32 246
Fragments arranged by size:
372 246 171 32 17 17 15
HindIII A'AGCT_T
Cuts at: 0 832 870
Size: 832 38
MfeI C'AATT_G
Cuts at: 0 593 870
Size: 593 277
MslI CAynn'nnrTG
Cuts at: 0 310 870
Size: 310 560
NcoI C'CATG_G
Cuts at: 0 140 870
Size: 140 730
PsiI TTA'TAA
Cuts at: 0 737 870
Size: 737 133
PstI C_TGCA'G
Cuts at: 0 332 629 870
Size: 332 297 241
Fragments arranged by size:
332 297 241
SbfI CC_TGCA'GG
Cuts at: 0 332 870
Size: 332 538
SfcI C'TryA_G
Cuts at: 0 328 625 870
Size: 328 297 245
Fragments arranged by size:
328 297 245
SmlI C'TyrA_G
Cuts at: 0 580 870
Size: 580 290
SpeI A'CTAG_T
Cuts at: 0 652 870
Size: 652 218
SspI AAT'ATT
Cuts at: 0 816 870
Size: 816 54
StyI C'CwwG_G
Cuts at: 0 140 870
Size: 140 730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 733 870
Size: 733 137
TatI w'GTAC_w
Cuts at: 0 224 503 870
Size: 224 279 367
Fragments arranged by size:
367 279 224
XbaI T'CTAG_A
Cuts at: 0 480 870
Size: 480 390
XmnI GAAnn'nnTTC
Cuts at: 0 469 521 524 870
Size: 469 52 3 346
Fragments arranged by size:
469 346 52 3
Enzymes that do cut:
AarI AflII AlwNI ApoI BanI BanII BbsI BclI
BglII Bme1580I BmrI BpmI BsaI BsaXI BseYI Bsp1286I
BspMI BstEII BstXI BstYI Bsu36I BtgI EarI Eco57MI
FalI Hin4I HindIII MfeI MslI NcoI PsiI PstI
SbfI SfcI SmlI SpeI SspI StyI TaqII TatI
XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TstI Tth111I XcmI XhoI
XmaI ZraI