Restriction map for
pFN21AE6656
(Linear) (Six-base) MAPSORT of: KIEE6656.seq Check: 5420 from: 1 to: 777
KIEE6656.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 777
Size: 3 774
ApaI G_GGCC'C
Cuts at: 0 360 777
Size: 360 417
ApoI r'AATT_y
Cuts at: 0 399 777
Size: 399 378
AvaI C'yCGr_G
Cuts at: 0 38 593 777
Size: 38 555 184
Fragments arranged by size:
555 184 38
BanII G_rGCy'C
Cuts at: 0 360 777
Size: 360 417
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 777
Size: 440 337
Bme1580I G_kGCm'C
Cuts at: 0 360 777
Size: 360 417
BmgBI CAC'GTC
Cuts at: 0 630 777
Size: 630 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 57 518 777
Size: 57 461 259
Fragments arranged by size:
461 259 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 500 518 777
Size: 163 337 18 259
Fragments arranged by size:
337 259 163 18
Bpu10I CC'TnA_GC
Cuts at: 0 154 777
Size: 154 623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 754 777
Size: 223 531 23
Fragments arranged by size:
531 223 23
BsaHI Gr'CG_yC
Cuts at: 0 5 777
Size: 5 772
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 430 460 473 503 777
Size: 34 30 366 30 13 30 274
Fragments arranged by size:
366 274 34 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 497 500 554 777
Size: 497 3 54 223
Fragments arranged by size:
497 223 54 3
BseYI C'CCAG_C
Cuts at: 0 359 777
Size: 359 418
BsiHKAI G_wGCw'C
Cuts at: 0 27 777
Size: 27 750
Bsp1286I G_dGCh'C
Cuts at: 0 27 360 777
Size: 27 333 417
Fragments arranged by size:
417 333 27
BspHI T'CATG_A
Cuts at: 0 395 777
Size: 395 382
BsrBI CCG'CTC
Cuts at: 0 45 777
Size: 45 732
BsrFI r'CCGG_y
Cuts at: 0 461 777
Size: 461 316
BssSI C'ACGA_G
Cuts at: 0 718 777
Size: 718 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 582 777
Size: 582 195
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 777
Size: 10 767
EaeI y'GGCC_r
Cuts at: 0 84 777
Size: 84 693
EarI CTCTTCn'nnn_
Cuts at: 0 83 658 777
Size: 83 575 119
Fragments arranged by size:
575 119 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 163 500 518 777
Size: 3 160 337 18 259
Fragments arranged by size:
337 259 160 18 3
HaeII r_GCGC'y
Cuts at: 0 687 777
Size: 687 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 430 462 473 505 777
Size: 239 32 159 32 11 32 272
Fragments arranged by size:
272 239 159 32 32 32 11
HindIII A'AGCT_T
Cuts at: 0 169 777
Size: 169 608
MscI TGG'CCA
Cuts at: 0 86 777
Size: 86 691
NaeI GCC'GGC
Cuts at: 0 463 777
Size: 463 314
NgoMIV G'CCGG_C
Cuts at: 0 461 777
Size: 461 316
PasI CC'CwG_GG
Cuts at: 0 694 777
Size: 694 83
PfoI T'CCnGG_A
Cuts at: 0 534 777
Size: 534 243
PspOMI G'GGCC_C
Cuts at: 0 356 777
Size: 356 421
PspXI vC'TCGA_Gb
Cuts at: 0 593 777
Size: 593 184
PstI C_TGCA'G
Cuts at: 0 98 777
Size: 98 679
SfcI C'TryA_G
Cuts at: 0 94 777
Size: 94 683
SmaI CCC'GGG
Cuts at: 0 40 777
Size: 40 737
SmlI C'TyrA_G
Cuts at: 0 202 593 769 777
Size: 202 391 176 8
Fragments arranged by size:
391 202 176 8
StuI AGG'CCT
Cuts at: 0 677 777
Size: 677 100
StyI C'CwwG_G
Cuts at: 0 159 777
Size: 159 618
TatI w'GTAC_w
Cuts at: 0 284 777
Size: 284 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 777
Size: 34 32 711
Fragments arranged by size:
711 34 32
Tth111I GACn'n_nGTC
Cuts at: 0 301 631 777
Size: 301 330 146
Fragments arranged by size:
330 301 146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 157 777
Size: 157 620
XhoI C'TCGA_G
Cuts at: 0 593 777
Size: 593 184
XmaI C'CCGG_G
Cuts at: 0 38 777
Size: 38 739
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanII BbsI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssSI BstAPI DrdI
EaeI EarI Eco57MI HaeII Hin4I HindIII MscI NaeI
NgoMIV PasI PfoI PspOMI PspXI PstI SfcI SmaI
SmlI StuI StyI TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrGI BssHII BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XmnI ZraI