Restriction map for
pFN21AE6654
(Linear) (Six-base) MAPSORT of: KIEE6654.seq Check: 3330 from: 1 to: 732
KIEE6654.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 553 732
Size: 553 179
AccI GT'mk_AC
Cuts at: 0 443 732
Size: 443 289
AleI CACnn'nnGTG
Cuts at: 0 541 732
Size: 541 191
AlwNI CAG_nnn'CTG
Cuts at: 0 78 732
Size: 78 654
ApaI G_GGCC'C
Cuts at: 0 226 506 732
Size: 226 280 226
Fragments arranged by size:
280 226 226
AvaI C'yCGr_G
Cuts at: 0 153 371 499 732
Size: 153 218 128 233
Fragments arranged by size:
233 218 153 128
BanI G'GyrC_C
Cuts at: 0 460 732
Size: 460 272
BanII G_rGCy'C
Cuts at: 0 226 500 506 732
Size: 226 274 6 226
Fragments arranged by size:
274 226 226 6
BbvCI CC'TCA_GC
Cuts at: 0 629 732
Size: 629 103
Bme1580I G_kGCm'C
Cuts at: 0 154 226 465 506 732
Size: 154 72 239 41 226
Fragments arranged by size:
239 226 154 72 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 198 732
Size: 198 534
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 134 732
Size: 134 598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 387 591 732
Size: 298 89 204 141
Fragments arranged by size:
298 204 141 89
Bpu10I CC'TnA_GC
Cuts at: 0 629 732
Size: 629 103
BsaAI yAC'GTr
Cuts at: 0 642 732
Size: 642 90
BsaHI Gr'CG_yC
Cuts at: 0 550 732
Size: 550 182
BseYI C'CCAG_C
Cuts at: 0 210 416 732
Size: 210 206 316
Fragments arranged by size:
316 210 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 732
Size: 64 668
BsiHKAI G_wGCw'C
Cuts at: 0 415 500 641 732
Size: 415 85 141 91
Fragments arranged by size:
415 141 91 85
BsmI GAATG_Cn'
Cuts at: 0 528 732
Size: 528 204
Bsp1286I G_dGCh'C
Cuts at: 0 154 226 415 465 500 506 641 732
Size: 154 72 189 50 35 6 135 91
Fragments arranged by size:
189 154 135 91 72 50 35 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 329 732
Size: 242 87 403
Fragments arranged by size:
403 242 87
BsrBI CCG'CTC
Cuts at: 0 204 732
Size: 204 528
BsrFI r'CCGG_y
Cuts at: 0 705 732
Size: 705 27
BssHII G'CGCG_C
Cuts at: 0 92 732
Size: 92 640
BstEII G'GTnAC_C
Cuts at: 0 33 282 732
Size: 33 249 450
Fragments arranged by size:
450 249 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 732
Size: 486 246
BstYI r'GATC_y
Cuts at: 0 126 304 512 732
Size: 126 178 208 220
Fragments arranged by size:
220 208 178 126
BtgI C'CryG_G
Cuts at: 0 227 554 689 732
Size: 227 327 135 43
Fragments arranged by size:
327 227 135 43
DraIII CAC_nnn'GTG
Cuts at: 0 645 732
Size: 645 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 139 732
Size: 139 593
EaeI y'GGCC_r
Cuts at: 0 353 732
Size: 353 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 732
Size: 139 593
EcoICRI GAG'CTC
Cuts at: 0 498 732
Size: 498 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 387 591 732
Size: 298 89 204 141
Fragments arranged by size:
298 204 141 89
EcoO109I rG'GnC_Cy
Cuts at: 0 223 503 732
Size: 223 280 229
Fragments arranged by size:
280 229 223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 732
Size: 312 420
HaeII r_GCGC'y
Cuts at: 0 79 211 732
Size: 79 132 521
Fragments arranged by size:
521 132 79
MscI TGG'CCA
Cuts at: 0 355 732
Size: 355 377
MslI CAynn'nnrTG
Cuts at: 0 337 541 732
Size: 337 204 191
Fragments arranged by size:
337 204 191
MspA1I CmG'CkG
Cuts at: 0 253 420 732
Size: 253 167 312
Fragments arranged by size:
312 253 167
NcoI C'CATG_G
Cuts at: 0 554 732
Size: 554 178
NspI r_CATG'y
Cuts at: 0 474 732
Size: 474 258
PasI CC'CwG_GG
Cuts at: 0 382 732
Size: 382 350
PfoI T'CCnGG_A
Cuts at: 0 275 732
Size: 275 457
PmlI CAC'GTG
Cuts at: 0 642 732
Size: 642 90
PspOMI G'GGCC_C
Cuts at: 0 222 502 732
Size: 222 280 230
Fragments arranged by size:
280 230 222
PstI C_TGCA'G
Cuts at: 0 269 732
Size: 269 463
SacI G_AGCT'C
Cuts at: 0 500 732
Size: 500 232
SfcI C'TryA_G
Cuts at: 0 265 732
Size: 265 467
SmaI CCC'GGG
Cuts at: 0 155 373 732
Size: 155 218 359
Fragments arranged by size:
359 218 155
StuI AGG'CCT
Cuts at: 0 295 732
Size: 295 437
StyI C'CwwG_G
Cuts at: 0 287 455 479 554 732
Size: 287 168 24 75 178
Fragments arranged by size:
287 178 168 75 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 689 732
Size: 172 517 43
Fragments arranged by size:
517 172 43
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 705 732
Size: 462 243 27
Fragments arranged by size:
462 243 27
XmaI C'CCGG_G
Cuts at: 0 153 371 732
Size: 153 218 361
Fragments arranged by size:
361 218 153
ZraI GAC'GTC
Cuts at: 0 551 732
Size: 551 181
Enzymes that do cut:
AatII AccI AleI AlwNI ApaI AvaI BanI BanII
BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BssHII BstEII BstXI BstYI BtgI DraIII DrdI EaeI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII MscI MslI
MspA1I NcoI NspI PasI PfoI PmlI PspOMI PstI
SacI SfcI SmaI StuI StyI TaqII XcmI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EagI EarI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmnI