Restriction map for
pFN21AE6645
(Linear) (Six-base) MAPSORT of: KIEE6645.seq Check: 6621 from: 1 to: 855
KIEE6645.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 192 350 416 835 855
Size: 164 28 158 66 419 20
Fragments arranged by size:
419 164 158 66 28 20
AlwNI CAG_nnn'CTG
Cuts at: 0 264 855
Size: 264 591
ApoI r'AATT_y
Cuts at: 0 292 582 855
Size: 292 290 273
Fragments arranged by size:
292 290 273
BamHI G'GATC_C
Cuts at: 0 659 855
Size: 659 196
BanII G_rGCy'C
Cuts at: 0 47 122 855
Size: 47 75 733
Fragments arranged by size:
733 75 47
BbvCI CC'TCA_GC
Cuts at: 0 637 855
Size: 637 218
BclI T'GATC_A
Cuts at: 0 176 855
Size: 176 679
BfrBI ATG'CAT
Cuts at: 0 249 855
Size: 249 606
BglII A'GATC_T
Cuts at: 0 737 855
Size: 737 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 286 608 855
Size: 144 142 322 247
Fragments arranged by size:
322 247 144 142
Bpu10I CC'TnA_GC
Cuts at: 0 637 855
Size: 637 218
BsaBI GATnn'nnATC
Cuts at: 0 233 855
Size: 233 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 419 449 855
Size: 419 30 406
Fragments arranged by size:
419 406 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 47 855
Size: 47 808
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 855
Size: 354 501
Bsp1286I G_dGCh'C
Cuts at: 0 47 122 855
Size: 47 75 733
Fragments arranged by size:
733 75 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 618 855
Size: 618 237
BsrBI CCG'CTC
Cuts at: 0 41 855
Size: 41 814
BsrDI GCAATG_nn'
Cuts at: 0 839 855
Size: 839 16
BstYI r'GATC_y
Cuts at: 0 594 659 737 855
Size: 594 65 78 118
Fragments arranged by size:
594 118 78 65
BtsI GCAGTG_nn'
Cuts at: 0 342 855
Size: 342 513
DraIII CAC_nnn'GTG
Cuts at: 0 332 855
Size: 332 523
EaeI y'GGCC_r
Cuts at: 0 444 744 855
Size: 444 300 111
Fragments arranged by size:
444 300 111
EarI CTCTTCn'nnn_
Cuts at: 0 54 714 855
Size: 54 660 141
Fragments arranged by size:
660 141 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 164 192 286 350 416 608 835
Size: 144 20 28 94 64 66 192 227
Cuts at: 835 855
Size: 20
Fragments arranged by size:
227 192 144 94 66 64 28 20
20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 146 855
Size: 146 709
EcoO109I rG'GnC_Cy
Cuts at: 0 261 855
Size: 261 594
EcoRI G'AATT_C
Cuts at: 0 582 855
Size: 582 273
EcoRV GAT'ATC
Cuts at: 0 688 855
Size: 688 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 855
Size: 651 32 172
Fragments arranged by size:
651 172 32
HindIII A'AGCT_T
Cuts at: 0 477 855
Size: 477 378
MscI TGG'CCA
Cuts at: 0 446 746 855
Size: 446 300 109
Fragments arranged by size:
446 300 109
MslI CAynn'nnrTG
Cuts at: 0 455 855
Size: 455 400
NsiI A_TGCA'T
Cuts at: 0 251 855
Size: 251 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 855
Size: 264 591
PfoI T'CCnGG_A
Cuts at: 0 131 263 855
Size: 131 132 592
Fragments arranged by size:
592 132 131
PpuMI rG'GwC_Cy
Cuts at: 0 261 855
Size: 261 594
PsiI TTA'TAA
Cuts at: 0 568 855
Size: 568 287
ScaI AGT'ACT
Cuts at: 0 511 855
Size: 511 344
SfcI C'TryA_G
Cuts at: 0 602 855
Size: 602 253
SspI AAT'ATT
Cuts at: 0 766 855
Size: 766 89
TatI w'GTAC_w
Cuts at: 0 509 855
Size: 509 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 855
Size: 726 32 97
Fragments arranged by size:
726 97 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 805 855
Size: 805 50
Enzymes that do cut:
AcuI AlwNI ApoI BamHI BanII BbvCI BclI BfrBI
BglII BpmI Bpu10I BsaBI BsaXI BseRI BsgI Bsp1286I
BspMI BsrBI BsrDI BstYI BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HindIII MscI
MslI NsiI PflMI PfoI PpuMI PsiI ScaI SfcI
SspI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI