Restriction map for
pFN21AE6642
(Linear) (Six-base) MAPSORT of: KIEE6642.seq Check: 4484 from: 1 to: 846
KIEE6642.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AfeI AGC'GCT
Cuts at: 0 324 846
Size: 324 522
AlwNI CAG_nnn'CTG
Cuts at: 0 200 846
Size: 200 646
ApoI r'AATT_y
Cuts at: 0 686 846
Size: 686 160
BanI G'GyrC_C
Cuts at: 0 76 846
Size: 76 770
BbeI G_GCGC'C
Cuts at: 0 80 846
Size: 80 766
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 846
Size: 584 262
BbvCI CC'TCA_GC
Cuts at: 0 281 846
Size: 281 565
BclI T'GATC_A
Cuts at: 0 716 846
Size: 716 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 248 846
Size: 248 598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 846
Size: 156 690
Bpu10I CC'TnA_GC
Cuts at: 0 281 846
Size: 281 565
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 846
Size: 54 792
BsaHI Gr'CG_yC
Cuts at: 0 77 846
Size: 77 769
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 846
Size: 154 30 662
Fragments arranged by size:
662 154 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 846
Size: 52 794
BseYI C'CCAG_C
Cuts at: 0 19 846
Size: 19 827
BsmI GAATG_Cn'
Cuts at: 0 246 846
Size: 246 600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 572 846
Size: 572 274
BsrBI CCG'CTC
Cuts at: 0 82 361 846
Size: 82 279 485
Fragments arranged by size:
485 279 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 252 846
Size: 27 225 594
Fragments arranged by size:
594 225 27
Bsu36I CC'TnA_GG
Cuts at: 0 643 846
Size: 643 203
BtgI C'CryG_G
Cuts at: 0 488 846
Size: 488 358
BtsI GCAGTG_nn'
Cuts at: 0 163 300 846
Size: 163 137 546
Fragments arranged by size:
546 163 137
EaeI y'GGCC_r
Cuts at: 0 375 510 846
Size: 375 135 336
Fragments arranged by size:
375 336 135
EarI CTCTTCn'nnn_
Cuts at: 0 107 730 736 846
Size: 107 623 6 110
Fragments arranged by size:
623 110 107 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 612 846
Size: 612 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 846
Size: 156 690
EcoRI G'AATT_C
Cuts at: 0 686 846
Size: 686 160
HaeII r_GCGC'y
Cuts at: 0 26 80 326 846
Size: 26 54 246 520
Fragments arranged by size:
520 246 54 26
KasI G'GCGC_C
Cuts at: 0 76 846
Size: 76 770
MscI TGG'CCA
Cuts at: 0 377 846
Size: 377 469
MslI CAynn'nnrTG
Cuts at: 0 349 709 724 846
Size: 349 360 15 122
Fragments arranged by size:
360 349 122 15
MspA1I CmG'CkG
Cuts at: 0 778 846
Size: 778 68
NarI GG'CG_CC
Cuts at: 0 77 846
Size: 77 769
NcoI C'CATG_G
Cuts at: 0 488 846
Size: 488 358
PasI CC'CwG_GG
Cuts at: 0 67 846
Size: 67 779
PstI C_TGCA'G
Cuts at: 0 158 569 846
Size: 158 411 277
Fragments arranged by size:
411 277 158
PvuII CAG'CTG
Cuts at: 0 778 846
Size: 778 68
SfcI C'TryA_G
Cuts at: 0 154 565 846
Size: 154 411 281
Fragments arranged by size:
411 281 154
SfoI GGC'GCC
Cuts at: 0 78 846
Size: 78 768
StyI C'CwwG_G
Cuts at: 0 94 488 846
Size: 94 394 358
Fragments arranged by size:
394 358 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 464 846
Size: 464 382
TatI w'GTAC_w
Cuts at: 0 257 846
Size: 257 589
Tth111I GACn'n_nGTC
Cuts at: 0 496 846
Size: 496 350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 458 846
Size: 458 388
Enzymes that do cut:
AfeI AlwNI ApoI BanI BbeI BbsI BbvCI BclI
BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsmI BspMI BsrBI BstXI Bsu36I BtgI BtsI EaeI
EarI EciI Eco57MI EcoRI HaeII KasI MscI MslI
MspA1I NarI NcoI PasI PstI PvuII SfcI SfoI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XhoI XmaI XmnI ZraI