(Linear) (Six-base) MAPSORT of: KIEE6641.seq  Check: 8562  from: 1  to: 777



KIEE6641.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AfeI AGC'GCT

Cuts at:      0     325     777 
   Size:        325     452
 
AhdI GACnn_n'nnGTC

Cuts at:      0     140     777 
   Size:        140     637
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     634     777 
   Size:        634     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391     777 
   Size:        391     386
 
ApoI r'AATT_y

Cuts at:      0     677     777 
   Size:        677     100
 
BanII G_rGCy'C

Cuts at:      0      76     777 
   Size:         76     701
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     504     777 
   Size:        504     273
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     427     461     777 
   Size:        427      34     316

  Fragments arranged by size: 

                 427     316      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     391     777 
   Size:        391     386
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     347     379     777 
   Size:        347      32     398

  Fragments arranged by size: 

                 398     347      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     533     777 
   Size:        533     244
 
BsaHI Gr'CG_yC

Cuts at:      0     136     777 
   Size:        136     641
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     605     635     777 
   Size:        605      30     142

  Fragments arranged by size: 

                 605     142      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     732     777 
   Size:        732      45
 
BsiHKAI G_wGCw'C

Cuts at:      0      76     588     623     777 
   Size:         76     512      35     154

  Fragments arranged by size: 

                 512     154      76      35
 
BsmI GAATG_Cn'

Cuts at:      0     184     777 
   Size:        184     593
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     127     777 
   Size:        127     650
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     588     623     777 
   Size:         76     512      35     154

  Fragments arranged by size: 

                 512     154      76      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     347     777 
   Size:        347     430
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     337     777 
   Size:        337     440
 
BtgI C'CryG_G

Cuts at:      0      53     453     777 
   Size:         53     400     324

  Fragments arranged by size: 

                 400     324      53
 
BtsI GCAGTG_nn'

Cuts at:      0     243     777 
   Size:        243     534
 
ClaI AT'CG_AT

Cuts at:      0     156     777 
   Size:        156     621
 
EaeI y'GGCC_r

Cuts at:      0       2      50     777 
   Size:          2      48     727

  Fragments arranged by size: 

                 727      48       2
 
EcoICRI GAG'CTC

Cuts at:      0      74     777 
   Size:         74     703
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     217     777 
   Size:        217     560
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705     777 
   Size:        705      72
 
HaeII r_GCGC'y

Cuts at:      0     327     777 
   Size:        327     450
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     777 
   Size:        126      32     619

  Fragments arranged by size: 

                 619     126      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     777 
   Size:        289     488
 
MslI CAynn'nnrTG

Cuts at:      0     335     511     777 
   Size:        335     176     266

  Fragments arranged by size: 

                 335     266     176
 
MspA1I CmG'CkG

Cuts at:      0      55     362     777 
   Size:         55     307     415

  Fragments arranged by size: 

                 415     307      55
 
NspI r_CATG'y

Cuts at:      0     171     345     777 
   Size:        171     174     432

  Fragments arranged by size: 

                 432     174     171
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     180     477     777 
   Size:        180     297     300

  Fragments arranged by size: 

                 300     297     180
 
SacI G_AGCT'C

Cuts at:      0      76     777 
   Size:         76     701
 
SacII CC_GC'GG

Cuts at:      0      56     777 
   Size:         56     721
 
StuI AGG'CCT

Cuts at:      0     223     777 
   Size:        223     554
 
StyI C'CwwG_G

Cuts at:      0     174     282     777 
   Size:        174     108     495

  Fragments arranged by size: 

                 495     174     108
 

 Enzymes that do cut:

     AfeI     AhdI     AlfI    AlwNI     ApoI    BanII     BbsI     BcgI
    BciVI     BmrI     BsaI    BsaHI    BsaXI    BseRI  BsiHKAI     BsmI
    BsmBI Bsp1286I   BstAPI    BstXI     BtgI     BtsI     ClaI     EaeI
  EcoICRI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MslI   MspA1I
     NspI    PflMI     SacI    SacII     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI