Restriction map for
pFN21AE6635
(Linear) (Six-base) MAPSORT of: KIEE6635.seq Check: 6496 from: 1 to: 741
KIEE6635.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
Acc65I G'GTAC_C
Cuts at: 0 393 741
Size: 393 348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 741
Size: 311 430
AflII C'TTAA_G
Cuts at: 0 130 741
Size: 130 611
ApoI r'AATT_y
Cuts at: 0 112 162 226 348 741
Size: 112 50 64 122 393
Fragments arranged by size:
393 122 112 64 50
AvaI C'yCGr_G
Cuts at: 0 26 741
Size: 26 715
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 204 237 385 418 741
Size: 204 33 148 33 323
Fragments arranged by size:
323 204 148 33 33
BamHI G'GATC_C
Cuts at: 0 645 741
Size: 645 96
BanI G'GyrC_C
Cuts at: 0 51 393 741
Size: 51 342 348
Fragments arranged by size:
348 342 51
BbeI G_GCGC'C
Cuts at: 0 55 741
Size: 55 686
BciVI GTATCCnnnnn_n'
Cuts at: 0 205 741
Size: 205 536
BfrBI ATG'CAT
Cuts at: 0 490 741
Size: 490 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 741
Size: 237 504
BsaHI Gr'CG_yC
Cuts at: 0 52 741
Size: 52 689
BseYI C'CCAG_C
Cuts at: 0 622 741
Size: 622 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 638 741
Size: 626 12 103
Fragments arranged by size:
626 103 12
BsiHKAI G_wGCw'C
Cuts at: 0 67 570 741
Size: 67 503 171
Fragments arranged by size:
503 171 67
Bsp1286I G_dGCh'C
Cuts at: 0 67 570 741
Size: 67 503 171
Fragments arranged by size:
503 171 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 508 705 741
Size: 508 197 36
Fragments arranged by size:
508 197 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 479 657 741
Size: 479 178 84
Fragments arranged by size:
479 178 84
BstYI r'GATC_y
Cuts at: 0 645 741
Size: 645 96
BtgI C'CryG_G
Cuts at: 0 397 741
Size: 397 344
DraI TTT'AAA
Cuts at: 0 495 741
Size: 495 246
EaeI y'GGCC_r
Cuts at: 0 308 443 741
Size: 308 135 298
Fragments arranged by size:
308 298 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 311 741
Size: 237 74 430
Fragments arranged by size:
430 237 74
EcoO109I rG'GnC_Cy
Cuts at: 0 82 741
Size: 82 659
HaeII r_GCGC'y
Cuts at: 0 20 34 55 741
Size: 20 14 21 686
Fragments arranged by size:
686 21 20 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 741
Size: 372 32 337
Fragments arranged by size:
372 337 32
HincII GTy'rAC
Cuts at: 0 681 741
Size: 681 60
KasI G'GCGC_C
Cuts at: 0 51 741
Size: 51 690
KpnI G_GTAC'C
Cuts at: 0 397 741
Size: 397 344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 522 741
Size: 189 333 219
Fragments arranged by size:
333 219 189
MscI TGG'CCA
Cuts at: 0 310 445 741
Size: 310 135 296
Fragments arranged by size:
310 296 135
MslI CAynn'nnrTG
Cuts at: 0 512 741
Size: 512 229
MspA1I CmG'CkG
Cuts at: 0 622 664 741
Size: 622 42 77
Fragments arranged by size:
622 77 42
NarI GG'CG_CC
Cuts at: 0 52 741
Size: 52 689
NcoI C'CATG_G
Cuts at: 0 397 741
Size: 397 344
NsiI A_TGCA'T
Cuts at: 0 492 741
Size: 492 249
PfoI T'CCnGG_A
Cuts at: 0 155 741
Size: 155 586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 323 355 569 601 741
Size: 323 32 214 32 140
Fragments arranged by size:
323 214 140 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 82 741
Size: 82 659
PshAI GACnn'nnGTC
Cuts at: 0 633 741
Size: 633 108
PvuII CAG'CTG
Cuts at: 0 622 664 741
Size: 622 42 77
Fragments arranged by size:
622 77 42
SanDI GG'GwC_CC
Cuts at: 0 82 741
Size: 82 659
SexAI A'CCwGG_T
Cuts at: 0 462 741
Size: 462 279
SfoI GGC'GCC
Cuts at: 0 53 741
Size: 53 688
SmlI C'TyrA_G
Cuts at: 0 130 741
Size: 130 611
SpeI A'CTAG_T
Cuts at: 0 537 741
Size: 537 204
SspI AAT'ATT
Cuts at: 0 104 741
Size: 104 637
StyI C'CwwG_G
Cuts at: 0 305 397 741
Size: 305 92 344
Fragments arranged by size:
344 305 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 741
Size: 588 32 121
Fragments arranged by size:
588 121 32
XmnI GAAnn'nnTTC
Cuts at: 0 151 741
Size: 151 590
Enzymes that do cut:
Acc65I AcuI AflII ApoI AvaI BaeI BamHI BanI
BbeI BciVI BfrBI BpmI BsaHI BseYI BsgI BsiHKAI
Bsp1286I BstAPI BstXI BstYI BtgI DraI EaeI Eco57MI
EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NsiI PfoI PpiI PpuMI
PshAI PvuII SanDI SexAI SfoI SmlI SpeI SspI
StyI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BanII BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
ZraI