Restriction map for
pFN21AE6634
(Linear) (Six-base) MAPSORT of: KIEE6634.seq Check: 7382 from: 1 to: 648
KIEE6634.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 162 648
Size: 162 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 376 648
Size: 205 171 272
Fragments arranged by size:
272 205 171
AlwNI CAG_nnn'CTG
Cuts at: 0 156 216 336 345 648
Size: 156 60 120 9 303
Fragments arranged by size:
303 156 120 60 9
ApaI G_GGCC'C
Cuts at: 0 393 648
Size: 393 255
BanI G'GyrC_C
Cuts at: 0 99 174 498 648
Size: 99 75 324 150
Fragments arranged by size:
324 150 99 75
BanII G_rGCy'C
Cuts at: 0 393 648
Size: 393 255
BbvCI CC'TCA_GC
Cuts at: 0 640 648
Size: 640 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 60 648
Size: 26 34 588
Fragments arranged by size:
588 34 26
Bme1580I G_kGCm'C
Cuts at: 0 104 393 648
Size: 104 289 255
Fragments arranged by size:
289 255 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 648
Size: 445 203
Bpu10I CC'TnA_GC
Cuts at: 0 640 648
Size: 640 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 458 488 648
Size: 458 30 160
Fragments arranged by size:
458 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 385 648
Size: 253 132 263
Fragments arranged by size:
263 253 132
BsiHKAI G_wGCw'C
Cuts at: 0 445 648
Size: 445 203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 461 648
Size: 88 373 187
Fragments arranged by size:
373 187 88
Bsp1286I G_dGCh'C
Cuts at: 0 104 393 445 648
Size: 104 289 52 203
Fragments arranged by size:
289 203 104 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 162 648
Size: 162 486
BsrFI r'CCGG_y
Cuts at: 0 614 648
Size: 614 34
BstBI TT'CG_AA
Cuts at: 0 60 648
Size: 60 588
BstEII G'GTnAC_C
Cuts at: 0 474 648
Size: 474 174
BstYI r'GATC_y
Cuts at: 0 431 534 648
Size: 431 103 114
Fragments arranged by size:
431 114 103
Bsu36I CC'TnA_GG
Cuts at: 0 269 503 648
Size: 269 234 145
Fragments arranged by size:
269 234 145
BtgI C'CryG_G
Cuts at: 0 479 648
Size: 479 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 648
Size: 300 348
EaeI y'GGCC_r
Cuts at: 0 218 648
Size: 218 430
EarI CTCTTCn'nnn_
Cuts at: 0 277 648
Size: 277 371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 376 445 648
Size: 205 171 69 203
Fragments arranged by size:
205 203 171 69
EcoO109I rG'GnC_Cy
Cuts at: 0 273 648
Size: 273 375
MscI TGG'CCA
Cuts at: 0 220 648
Size: 220 428
MspA1I CmG'CkG
Cuts at: 0 381 448 648
Size: 381 67 200
Fragments arranged by size:
381 200 67
NaeI GCC'GGC
Cuts at: 0 616 648
Size: 616 32
NcoI C'CATG_G
Cuts at: 0 479 648
Size: 479 169
NgoMIV G'CCGG_C
Cuts at: 0 614 648
Size: 614 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 648
Size: 216 432
PpuMI rG'GwC_Cy
Cuts at: 0 273 648
Size: 273 375
PspOMI G'GGCC_C
Cuts at: 0 389 648
Size: 389 259
PstI C_TGCA'G
Cuts at: 0 341 648
Size: 341 307
PvuII CAG'CTG
Cuts at: 0 381 648
Size: 381 267
SfcI C'TryA_G
Cuts at: 0 337 436 648
Size: 337 99 212
Fragments arranged by size:
337 212 99
StuI AGG'CCT
Cuts at: 0 592 648
Size: 592 56
StyI C'CwwG_G
Cuts at: 0 479 648
Size: 479 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 645 648
Size: 645 3
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 648
Size: 458 32 158
Fragments arranged by size:
458 158 32
XbaI T'CTAG_A
Cuts at: 0 234 648
Size: 234 414
Enzymes that do cut:
AarI AcuI AlwNI ApaI BanI BanII BbvCI BcgI
Bme1580I BpmI Bpu10I BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BstBI BstEII BstYI Bsu36I BtgI BtgZI
EaeI EarI Eco57MI EcoO109I MscI MspA1I NaeI NcoI
NgoMIV PflMI PpuMI PspOMI PstI PvuII SfcI StuI
StyI TaqII TstI XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XcmI XhoI XmaI XmnI ZraI