Restriction map for
pFN21AE6633
(Linear) (Six-base) MAPSORT of: KIEE6633.seq Check: 9416 from: 1 to: 948
KIEE6633.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 828 948
Size: 241 587 120
Fragments arranged by size:
587 241 120
AfeI AGC'GCT
Cuts at: 0 577 948
Size: 577 371
AlwNI CAG_nnn'CTG
Cuts at: 0 276 851 948
Size: 276 575 97
Fragments arranged by size:
575 276 97
ApaI G_GGCC'C
Cuts at: 0 882 948
Size: 882 66
ApaLI G'TGCA_C
Cuts at: 0 682 948
Size: 682 266
AvaI C'yCGr_G
Cuts at: 0 191 948
Size: 191 757
BanI G'GyrC_C
Cuts at: 0 136 658 948
Size: 136 522 290
Fragments arranged by size:
522 290 136
BanII G_rGCy'C
Cuts at: 0 176 192 827 882 948
Size: 176 16 635 55 66
Fragments arranged by size:
635 176 66 55 16
BbeI G_GCGC'C
Cuts at: 0 140 948
Size: 140 808
BbvCI CC'TCA_GC
Cuts at: 0 326 812 948
Size: 326 486 136
Fragments arranged by size:
486 326 136
BciVI GTATCCnnnnn_n'
Cuts at: 0 249 948
Size: 249 699
BclI T'GATC_A
Cuts at: 0 128 365 948
Size: 128 237 583
Fragments arranged by size:
583 237 128
BlpI GC'TnA_GC
Cuts at: 0 899 948
Size: 899 49
Bme1580I G_kGCm'C
Cuts at: 0 686 882 948
Size: 686 196 66
Fragments arranged by size:
686 196 66
BmtI G_CTAG'C
Cuts at: 0 182 948
Size: 182 766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 547 841 948
Size: 496 51 294 107
Fragments arranged by size:
496 294 107 51
Bpu10I CC'TnA_GC
Cuts at: 0 326 812 948
Size: 326 486 136
Fragments arranged by size:
486 326 136
BsaHI Gr'CG_yC
Cuts at: 0 137 692 948
Size: 137 555 256
Fragments arranged by size:
555 256 137
BsaWI w'CCGG_w
Cuts at: 0 938 948
Size: 938 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 948
Size: 179 30 739
Fragments arranged by size:
739 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 448 711 948
Size: 200 248 263 237
Fragments arranged by size:
263 248 237 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 485 781 948
Size: 124 361 296 167
Fragments arranged by size:
361 296 167 124
BsiHKAI G_wGCw'C
Cuts at: 0 176 686 948
Size: 176 510 262
Fragments arranged by size:
510 262 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 948
Size: 154 794
Bsp1286I G_dGCh'C
Cuts at: 0 176 192 686 827 882 948
Size: 176 16 494 141 55 66
Fragments arranged by size:
494 176 141 66 55 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 948
Size: 476 472
BsrBI CCG'CTC
Cuts at: 0 701 948
Size: 701 247
BssSI C'ACGA_G
Cuts at: 0 632 948
Size: 632 316
BstEII G'GTnAC_C
Cuts at: 0 6 666 948
Size: 6 660 282
Fragments arranged by size:
660 282 6
BtsI GCAGTG_nn'
Cuts at: 0 686 798 948
Size: 686 112 150
Fragments arranged by size:
686 150 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 867 948
Size: 867 81
EaeI y'GGCC_r
Cuts at: 0 164 614 641 948
Size: 164 450 27 307
Fragments arranged by size:
450 307 164 27
EarI CTCTTCn'nnn_
Cuts at: 0 754 948
Size: 754 194
EcoICRI GAG'CTC
Cuts at: 0 174 948
Size: 174 774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 496 547 828 841 948
Size: 241 255 51 281 13 107
Fragments arranged by size:
281 255 241 107 51 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 811 948
Size: 811 137
EcoO109I rG'GnC_Cy
Cuts at: 0 816 860 878 948
Size: 816 44 18 70
Fragments arranged by size:
816 70 44 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 567 948
Size: 567 381
HaeII r_GCGC'y
Cuts at: 0 140 579 906 948
Size: 140 439 327 42
Fragments arranged by size:
439 327 140 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 292 324 948
Size: 179 32 81 32 624
Fragments arranged by size:
624 179 81 32 32
HincII GTy'rAC
Cuts at: 0 438 948
Size: 438 510
KasI G'GCGC_C
Cuts at: 0 136 948
Size: 136 812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 948
Size: 323 625
MscI TGG'CCA
Cuts at: 0 616 643 948
Size: 616 27 305
Fragments arranged by size:
616 305 27
MspA1I CmG'CkG
Cuts at: 0 409 898 920 948
Size: 409 489 22 28
Fragments arranged by size:
489 409 28 22
NarI GG'CG_CC
Cuts at: 0 137 948
Size: 137 811
NheI G'CTAG_C
Cuts at: 0 178 948
Size: 178 770
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 948
Size: 276 672
PfoI T'CCnGG_A
Cuts at: 0 473 948
Size: 473 475
PpuMI rG'GwC_Cy
Cuts at: 0 860 948
Size: 860 88
PspOMI G'GGCC_C
Cuts at: 0 878 948
Size: 878 70
PstI C_TGCA'G
Cuts at: 0 211 948
Size: 211 737
PvuII CAG'CTG
Cuts at: 0 409 898 920 948
Size: 409 489 22 28
Fragments arranged by size:
489 409 28 22
SacI G_AGCT'C
Cuts at: 0 176 948
Size: 176 772
SapI GCTCTTCn'nnn_
Cuts at: 0 754 948
Size: 754 194
ScaI AGT'ACT
Cuts at: 0 841 948
Size: 841 107
SfcI C'TryA_G
Cuts at: 0 207 579 948
Size: 207 372 369
Fragments arranged by size:
372 369 207
SfoI GGC'GCC
Cuts at: 0 138 948
Size: 138 810
StyI C'CwwG_G
Cuts at: 0 11 654 874 948
Size: 11 643 220 74
Fragments arranged by size:
643 220 74 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 701 855 948
Size: 701 154 93
Fragments arranged by size:
701 154 93
TatI w'GTAC_w
Cuts at: 0 216 839 948
Size: 216 623 109
Fragments arranged by size:
623 216 109
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbvCI BciVI BclI BlpI Bme1580I BmtI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BssSI BstEII BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MmeI MscI MspA1I NarI NheI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI ScaI
SfcI SfoI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BfrBI BglI BglII
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BseYI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MslI NaeI NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI