Restriction map for
pFN21AE6632
(Linear) (Six-base) MAPSORT of: KIEE6632.seq Check: 4997 from: 1 to: 741
KIEE6632.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 272 741
Size: 272 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 741
Size: 233 508
BanII G_rGCy'C
Cuts at: 0 589 741
Size: 589 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 561 741
Size: 561 180
BbvCI CC'TCA_GC
Cuts at: 0 124 741
Size: 124 617
Bme1580I G_kGCm'C
Cuts at: 0 542 741
Size: 542 199
BmgBI CAC'GTC
Cuts at: 0 102 741
Size: 102 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 741
Size: 269 472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 262 316 454 496 741
Size: 211 51 54 138 42 245
Fragments arranged by size:
245 211 138 54 51 42
Bpu10I CC'TnA_GC
Cuts at: 0 124 741
Size: 124 617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 741
Size: 97 644
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 741
Size: 660 81
BsaBI GATnn'nnATC
Cuts at: 0 343 741
Size: 343 398
BsaHI Gr'CG_yC
Cuts at: 0 445 741
Size: 445 296
BsaWI w'CCGG_w
Cuts at: 0 622 741
Size: 622 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 648 741
Size: 112 536 93
Fragments arranged by size:
536 112 93
BseYI C'CCAG_C
Cuts at: 0 141 640 686 741
Size: 141 499 46 55
Fragments arranged by size:
499 141 55 46
BsiEI CG_ry'CG
Cuts at: 0 8 741
Size: 8 733
BsmI GAATG_Cn'
Cuts at: 0 711 741
Size: 711 30
Bsp1286I G_dGCh'C
Cuts at: 0 542 589 741
Size: 542 47 152
Fragments arranged by size:
542 152 47
BssSI C'ACGA_G
Cuts at: 0 37 741
Size: 37 704
BstYI r'GATC_y
Cuts at: 0 627 741
Size: 627 114
BtgI C'CryG_G
Cuts at: 0 266 741
Size: 266 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 663 741
Size: 663 78
DraI TTT'AAA
Cuts at: 0 189 741
Size: 189 552
EaeI y'GGCC_r
Cuts at: 0 5 741
Size: 5 736
EagI C'GGCC_G
Cuts at: 0 5 741
Size: 5 736
EarI CTCTTCn'nnn_
Cuts at: 0 700 741
Size: 700 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 741
Size: 28 713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 233 262 316 454 496 741
Size: 211 22 29 54 138 42 245
Fragments arranged by size:
245 211 138 54 42 29 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 741
Size: 469 272
EcoO109I rG'GnC_Cy
Cuts at: 0 644 741
Size: 644 97
MspA1I CmG'CkG
Cuts at: 0 690 741
Size: 690 51
NcoI C'CATG_G
Cuts at: 0 266 741
Size: 266 475
PpuMI rG'GwC_Cy
Cuts at: 0 644 741
Size: 644 97
PshAI GACnn'nnGTC
Cuts at: 0 268 457 741
Size: 268 189 284
Fragments arranged by size:
284 268 189
PvuII CAG'CTG
Cuts at: 0 690 741
Size: 690 51
RsrII CG'GwC_CG
Cuts at: 0 19 741
Size: 19 722
SapI GCTCTTCn'nnn_
Cuts at: 0 700 741
Size: 700 41
SfcI C'TryA_G
Cuts at: 0 273 366 455 741
Size: 273 93 89 286
Fragments arranged by size:
286 273 93 89
SmlI C'TyrA_G
Cuts at: 0 112 741
Size: 112 629
StuI AGG'CCT
Cuts at: 0 134 475 741
Size: 134 341 266
Fragments arranged by size:
341 266 134
StyI C'CwwG_G
Cuts at: 0 266 741
Size: 266 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 741
Size: 700 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 741
Size: 365 32 344
Fragments arranged by size:
365 344 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 302 351 741
Size: 302 49 390
Fragments arranged by size:
390 302 49
Enzymes that do cut:
AccI AcuI BanII BbsI BbvCI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsmI Bsp1286I BssSI BstYI BtgI BtgZI
DraI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
MspA1I NcoI PpuMI PshAI PvuII RsrII SapI SfcI
SmlI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI BsaAI BsaXI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PstI PvuI SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI