Restriction map for
pFN21AE6630
(Linear) (Six-base) MAPSORT of: KIEE6630.seq Check: 7629 from: 1 to: 771
KIEE6630.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AfeI AGC'GCT
Cuts at: 0 700 771
Size: 700 71
AlwNI CAG_nnn'CTG
Cuts at: 0 522 771
Size: 522 249
ApaLI G'TGCA_C
Cuts at: 0 91 771
Size: 91 680
ApoI r'AATT_y
Cuts at: 0 220 771
Size: 220 551
BanI G'GyrC_C
Cuts at: 0 558 771
Size: 558 213
Bme1580I G_kGCm'C
Cuts at: 0 95 771
Size: 95 676
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 771
Size: 182 589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 499 706 771
Size: 152 347 207 65
Fragments arranged by size:
347 207 152 65
Bpu10I CC'TnA_GC
Cuts at: 0 22 771
Size: 22 749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 490 771
Size: 274 216 281
Fragments arranged by size:
281 274 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 771
Size: 10 761
BsaAI yAC'GTr
Cuts at: 0 738 771
Size: 738 33
BsaBI GATnn'nnATC
Cuts at: 0 314 771
Size: 314 457
BsaWI w'CCGG_w
Cuts at: 0 619 771
Size: 619 152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 771
Size: 68 30 673
Fragments arranged by size:
673 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 722 771
Size: 722 49
BsiHKAI G_wGCw'C
Cuts at: 0 95 771
Size: 95 676
BsmBI CGTCTCn'nnnn_
Cuts at: 0 617 771
Size: 617 154
Bsp1286I G_dGCh'C
Cuts at: 0 95 771
Size: 95 676
BspEI T'CCGG_A
Cuts at: 0 619 771
Size: 619 152
BsrBI CCG'CTC
Cuts at: 0 84 771
Size: 84 687
BstBI TT'CG_AA
Cuts at: 0 216 265 771
Size: 216 49 506
Fragments arranged by size:
506 216 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 771
Size: 69 702
BstYI r'GATC_y
Cuts at: 0 422 771
Size: 422 349
DraI TTT'AAA
Cuts at: 0 186 771
Size: 186 585
EarI CTCTTCn'nnn_
Cuts at: 0 49 643 771
Size: 49 594 128
Fragments arranged by size:
594 128 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 499 706 771
Size: 152 347 207 65
Fragments arranged by size:
347 207 152 65
EcoO109I rG'GnC_Cy
Cuts at: 0 163 510 771
Size: 163 347 261
Fragments arranged by size:
347 261 163
HaeII r_GCGC'y
Cuts at: 0 702 771
Size: 702 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 771
Size: 476 32 263
Fragments arranged by size:
476 263 32
MfeI C'AATT_G
Cuts at: 0 245 771
Size: 245 526
MspA1I CmG'CkG
Cuts at: 0 172 535 771
Size: 172 363 236
Fragments arranged by size:
363 236 172
NspI r_CATG'y
Cuts at: 0 402 771
Size: 402 369
PflMI CCAn_nnn'nTGG
Cuts at: 0 522 771
Size: 522 249
PfoI T'CCnGG_A
Cuts at: 0 674 771
Size: 674 97
PpuMI rG'GwC_Cy
Cuts at: 0 510 771
Size: 510 261
PstI C_TGCA'G
Cuts at: 0 177 771
Size: 177 594
PvuII CAG'CTG
Cuts at: 0 172 535 771
Size: 172 363 236
Fragments arranged by size:
363 236 172
SfcI C'TryA_G
Cuts at: 0 173 636 771
Size: 173 463 135
Fragments arranged by size:
463 173 135
SmlI C'TyrA_G
Cuts at: 0 253 505 771
Size: 253 252 266
Fragments arranged by size:
266 253 252
StuI AGG'CCT
Cuts at: 0 553 691 771
Size: 553 138 80
Fragments arranged by size:
553 138 80
StyI C'CwwG_G
Cuts at: 0 554 771
Size: 554 217
Enzymes that do cut:
AfeI AlwNI ApaLI ApoI BanI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BsiHKAI BsmBI Bsp1286I BspEI BsrBI BstBI BstXI BstYI
DraI EarI Eco57MI EcoO109I HaeII Hin4I MfeI MspA1I
NspI PflMI PfoI PpuMI PstI PvuII SfcI SmlI
StuI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BsaHI BseYI BsgI BsiEI BsiWI
BsmI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI