Restriction map for
pFN21AE6629
(Linear) (Six-base) MAPSORT of: KIEE6629.seq Check: 3441 from: 1 to: 870
KIEE6629.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 870
Size: 298 572
AflIII A'CryG_T
Cuts at: 0 525 765 870
Size: 525 240 105
Fragments arranged by size:
525 240 105
ApoI r'AATT_y
Cuts at: 0 745 870
Size: 745 125
AvaI C'yCGr_G
Cuts at: 0 40 870
Size: 40 830
BanII G_rGCy'C
Cuts at: 0 419 870
Size: 419 451
BbvCI CC'TCA_GC
Cuts at: 0 757 870
Size: 757 113
BglI GCCn_nnn'nGGC
Cuts at: 0 318 870
Size: 318 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 363 654 870
Size: 363 291 216
Fragments arranged by size:
363 291 216
Bpu10I CC'TnA_GC
Cuts at: 0 10 757 870
Size: 10 747 113
Fragments arranged by size:
747 113 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 299 870
Size: 150 149 571
Fragments arranged by size:
571 150 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 870
Size: 421 30 419
Fragments arranged by size:
421 419 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 85 870
Size: 73 12 785
Fragments arranged by size:
785 73 12
BseYI C'CCAG_C
Cuts at: 0 728 870
Size: 728 142
BsiHKAI G_wGCw'C
Cuts at: 0 386 419 870
Size: 386 33 451
Fragments arranged by size:
451 386 33
Bsp1286I G_dGCh'C
Cuts at: 0 386 419 870
Size: 386 33 451
Fragments arranged by size:
451 386 33
BsrDI GCAATG_nn'
Cuts at: 0 307 494 870
Size: 307 187 376
Fragments arranged by size:
376 307 187
BstBI TT'CG_AA
Cuts at: 0 750 870
Size: 750 120
BtgI C'CryG_G
Cuts at: 0 410 870
Size: 410 460
BtsI GCAGTG_nn'
Cuts at: 0 687 870
Size: 687 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 203 238 429 464 870
Size: 203 35 191 35 406
Fragments arranged by size:
406 203 191 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 241 870
Size: 241 629
EarI CTCTTCn'nnn_
Cuts at: 0 44 62 354 870
Size: 44 18 292 516
Fragments arranged by size:
516 292 44 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 193 870
Size: 19 174 677
Fragments arranged by size:
677 174 19
EcoICRI GAG'CTC
Cuts at: 0 417 870
Size: 417 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 870
Size: 298 572
HindIII A'AGCT_T
Cuts at: 0 401 870
Size: 401 469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 870
Size: 176 694
NcoI C'CATG_G
Cuts at: 0 410 870
Size: 410 460
NspI r_CATG'y
Cuts at: 0 529 769 870
Size: 529 240 101
Fragments arranged by size:
529 240 101
PciI A'CATG_T
Cuts at: 0 525 765 870
Size: 525 240 105
Fragments arranged by size:
525 240 105
SacI G_AGCT'C
Cuts at: 0 419 870
Size: 419 451
SfcI C'TryA_G
Cuts at: 0 733 870
Size: 733 137
SmlI C'TyrA_G
Cuts at: 0 165 314 870
Size: 165 149 556
Fragments arranged by size:
556 165 149
StyI C'CwwG_G
Cuts at: 0 410 870
Size: 410 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 636 870
Size: 636 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 870
Size: 421 32 417
Fragments arranged by size:
421 417 32
Tth111I GACn'n_nGTC
Cuts at: 0 847 870
Size: 847 23
XmnI GAAnn'nnTTC
Cuts at: 0 746 870
Size: 746 124
Enzymes that do cut:
AcuI AflIII ApoI AvaI BanII BbvCI BglI BmrI
Bpu10I BpuEI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrDI
BstBI BtgI BtsI CspCI DrdI EarI EciI EcoICRI
Eco57MI HindIII MmeI NcoI NspI PciI SacI SfcI
SmlI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EaeI
EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI XbaI XcmI XhoI XmaI ZraI