Restriction map for
pFN21AE6616
(Linear) (Six-base) MAPSORT of: KIEE6616.seq Check: 4156 from: 1 to: 1005
KIEE6616.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 569 1005
Size: 569 436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 34 1005
Size: 28 6 971
Fragments arranged by size:
971 28 6
AflIII A'CryG_T
Cuts at: 0 20 1005
Size: 20 985
AlwNI CAG_nnn'CTG
Cuts at: 0 297 1005
Size: 297 708
ApaI G_GGCC'C
Cuts at: 0 685 1005
Size: 685 320
AvaI C'yCGr_G
Cuts at: 0 211 827 905 1005
Size: 211 616 78 100
Fragments arranged by size:
616 211 100 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 271 304 901 934 1005
Size: 271 33 597 33 71
Fragments arranged by size:
597 271 71 33 33
BamHI G'GATC_C
Cuts at: 0 867 1005
Size: 867 138
BanI G'GyrC_C
Cuts at: 0 306 1005
Size: 306 699
BanII G_rGCy'C
Cuts at: 0 685 734 985 1005
Size: 685 49 251 20
Fragments arranged by size:
685 251 49 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 841 1005
Size: 841 164
BbvCI CC'TCA_GC
Cuts at: 0 341 1005
Size: 341 664
BglI GCCn_nnn'nGGC
Cuts at: 0 654 738 1005
Size: 654 84 267
Fragments arranged by size:
654 267 84
Bme1580I G_kGCm'C
Cuts at: 0 311 685 1005
Size: 311 374 320
Fragments arranged by size:
374 320 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 901 1005
Size: 859 42 104
Fragments arranged by size:
859 104 42
Bpu10I CC'TnA_GC
Cuts at: 0 341 1005
Size: 341 664
BsaI GGTCTCn'nnnn_
Cuts at: 0 366 1005
Size: 366 639
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 379 628 970 1005
Size: 49 330 249 342 35
Fragments arranged by size:
342 330 249 49 35
BseYI C'CCAG_C
Cuts at: 0 155 197 425 452 733 990 1005
Size: 155 42 228 27 281 257 15
Fragments arranged by size:
281 257 228 155 42 27 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 1005
Size: 885 120
BsiEI CG_ry'CG
Cuts at: 0 50 702 1005
Size: 50 652 303
Fragments arranged by size:
652 303 50
Bsp1286I G_dGCh'C
Cuts at: 0 311 685 734 985 1005
Size: 311 374 49 251 20
Fragments arranged by size:
374 311 251 49 20
BsrBI CCG'CTC
Cuts at: 0 823 1005
Size: 823 182
BsrFI r'CCGG_y
Cuts at: 0 52 1005
Size: 52 953
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 1005
Size: 324 681
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 879 1005
Size: 357 522 126
Fragments arranged by size:
522 357 126
BstYI r'GATC_y
Cuts at: 0 141 867 1005
Size: 141 726 138
Fragments arranged by size:
726 141 138
Bsu36I CC'TnA_GG
Cuts at: 0 686 1005
Size: 686 319
BtgI C'CryG_G
Cuts at: 0 465 630 770 886 1005
Size: 465 165 140 116 119
Fragments arranged by size:
465 165 140 119 116
DraIII CAC_nnn'GTG
Cuts at: 0 405 864 1005
Size: 405 459 141
Fragments arranged by size:
459 405 141
EaeI y'GGCC_r
Cuts at: 0 81 178 699 1005
Size: 81 97 521 306
Fragments arranged by size:
521 306 97 81
EagI C'GGCC_G
Cuts at: 0 699 1005
Size: 699 306
EarI CTCTTCn'nnn_
Cuts at: 0 397 1005
Size: 397 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 34 859 901 1005
Size: 28 6 825 42 104
Fragments arranged by size:
825 104 42 28 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 252 1005
Size: 252 753
EcoO109I rG'GnC_Cy
Cuts at: 0 216 346 489 537 554 681 682 740
Size: 216 130 143 48 17 127 1 58
Cuts at: 740 876 1005
Size: 136 129
Fragments arranged by size:
216 143 136 130 129 127 58 48
17 1
MfeI C'AATT_G
Cuts at: 0 995 1005
Size: 995 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1005
Size: 198 807
MslI CAynn'nnrTG
Cuts at: 0 464 1005
Size: 464 541
MspA1I CmG'CkG
Cuts at: 0 159 357 429 480 963 969 1005
Size: 159 198 72 51 483 6 36
Fragments arranged by size:
483 198 159 72 51 36 6
NaeI GCC'GGC
Cuts at: 0 54 1005
Size: 54 951
NgoMIV G'CCGG_C
Cuts at: 0 52 1005
Size: 52 953
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 1005
Size: 864 141
PpuMI rG'GwC_Cy
Cuts at: 0 346 537 554 876 1005
Size: 346 191 17 322 129
Fragments arranged by size:
346 322 191 129 17
PspOMI G'GGCC_C
Cuts at: 0 681 1005
Size: 681 324
PstI C_TGCA'G
Cuts at: 0 968 1005
Size: 968 37
PvuI CG_AT'CG
Cuts at: 0 50 1005
Size: 50 955
PvuII CAG'CTG
Cuts at: 0 159 963 1005
Size: 159 804 42
Fragments arranged by size:
804 159 42
SanDI GG'GwC_CC
Cuts at: 0 346 554 1005
Size: 346 208 451
Fragments arranged by size:
451 346 208
SexAI A'CCwGG_T
Cuts at: 0 401 1005
Size: 401 604
SfcI C'TryA_G
Cuts at: 0 594 964 1005
Size: 594 370 41
Fragments arranged by size:
594 370 41
SmaI CCC'GGG
Cuts at: 0 829 1005
Size: 829 176
StyI C'CwwG_G
Cuts at: 0 558 872 1005
Size: 558 314 133
Fragments arranged by size:
558 314 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 1005
Size: 514 491
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 618 650 755 787 1005
Size: 618 32 105 32 218
Fragments arranged by size:
618 218 105 32 32
XmaI C'CCGG_G
Cuts at: 0 827 1005
Size: 827 178
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BglI Bme1580I BpmI Bpu10I
BsaI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I MfeI MmeI MslI MspA1I
NaeI NgoMIV PflMI PpuMI PspOMI PstI PvuI PvuII
SanDI SexAI SfcI SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmnI ZraI