Restriction map for
pFN21AE6608
(Linear) (Six-base) MAPSORT of: KIEE6608.seq Check: 6002 from: 1 to: 912
KIEE6608.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 769 912
Size: 516 253 143
Fragments arranged by size:
516 253 143
AflIII A'CryG_T
Cuts at: 0 198 912
Size: 198 714
ApoI r'AATT_y
Cuts at: 0 657 912
Size: 657 255
AvaI C'yCGr_G
Cuts at: 0 830 912
Size: 830 82
BanI G'GyrC_C
Cuts at: 0 518 912
Size: 518 394
BanII G_rGCy'C
Cuts at: 0 397 837 912
Size: 397 440 75
Fragments arranged by size:
440 397 75
BclI T'GATC_A
Cuts at: 0 80 912
Size: 80 832
Bme1580I G_kGCm'C
Cuts at: 0 521 912
Size: 521 391
BmgBI CAC'GTC
Cuts at: 0 16 199 912
Size: 16 183 713
Fragments arranged by size:
713 183 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 391 912
Size: 157 234 521
Fragments arranged by size:
521 234 157
BsaAI yAC'GTr
Cuts at: 0 805 912
Size: 805 107
BsaWI w'CCGG_w
Cuts at: 0 75 912
Size: 75 837
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 155 185 912
Size: 38 30 87 30 727
Fragments arranged by size:
727 87 38 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 510 912
Size: 510 402
BseYI C'CCAG_C
Cuts at: 0 384 912
Size: 384 528
Bsp1286I G_dGCh'C
Cuts at: 0 397 521 837 912
Size: 397 124 316 75
Fragments arranged by size:
397 316 124 75
BspHI T'CATG_A
Cuts at: 0 621 912
Size: 621 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 717 912
Size: 124 593 195
Fragments arranged by size:
593 195 124
BsrDI GCAATG_nn'
Cuts at: 0 866 912
Size: 866 46
BstYI r'GATC_y
Cuts at: 0 144 912
Size: 144 768
Bsu36I CC'TnA_GG
Cuts at: 0 493 912
Size: 493 419
BtsI GCAGTG_nn'
Cuts at: 0 779 912
Size: 779 133
EaeI y'GGCC_r
Cuts at: 0 765 890 912
Size: 765 125 22
Fragments arranged by size:
765 125 22
EarI CTCTTCn'nnn_
Cuts at: 0 535 875 912
Size: 535 340 37
Fragments arranged by size:
535 340 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 391 516 769 912
Size: 157 234 125 253 143
Fragments arranged by size:
253 234 157 143 125
EcoO109I rG'GnC_Cy
Cuts at: 0 113 525 912
Size: 113 412 387
Fragments arranged by size:
412 387 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 489 521 534 566 912
Size: 175 32 282 32 13 32 346
Fragments arranged by size:
346 282 175 32 32 32 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 159 282 912
Size: 24 135 123 630
Fragments arranged by size:
630 135 123 24
MscI TGG'CCA
Cuts at: 0 767 892 912
Size: 767 125 20
Fragments arranged by size:
767 125 20
MspA1I CmG'CkG
Cuts at: 0 665 912
Size: 665 247
NdeI CA'TA_TG
Cuts at: 0 731 912
Size: 731 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 912
Size: 141 771
PpuMI rG'GwC_Cy
Cuts at: 0 113 912
Size: 113 799
PvuII CAG'CTG
Cuts at: 0 665 912
Size: 665 247
SexAI A'CCwGG_T
Cuts at: 0 626 912
Size: 626 286
StyI C'CwwG_G
Cuts at: 0 816 912
Size: 816 96
TatI w'GTAC_w
Cuts at: 0 216 912
Size: 216 696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 912
Size: 38 32 842
Fragments arranged by size:
842 38 32
XbaI T'CTAG_A
Cuts at: 0 193 912
Size: 193 719
Enzymes that do cut:
AcuI AflIII ApoI AvaI BanI BanII BclI Bme1580I
BmgBI BpmI BsaAI BsaWI BsaXI BseRI BseYI Bsp1286I
BspHI BspMI BsrDI BstYI Bsu36I BtsI EaeI EarI
Eco57MI EcoO109I Hin4I MmeI MscI MspA1I NdeI PflMI
PpuMI PvuII SexAI StyI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XcmI XhoI XmaI XmnI ZraI