Restriction map for
pFN21AE6606
(Linear) (Six-base) MAPSORT of: KIEE6606.seq Check: 2875 from: 1 to: 1002
KIEE6606.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
Acc65I G'GTAC_C
Cuts at: 0 509 1002
Size: 509 493
AflIII A'CryG_T
Cuts at: 0 647 900 1002
Size: 647 253 102
Fragments arranged by size:
647 253 102
AleI CACnn'nnGTG
Cuts at: 0 292 616 1002
Size: 292 324 386
Fragments arranged by size:
386 324 292
AlwNI CAG_nnn'CTG
Cuts at: 0 13 1002
Size: 13 989
ApaI G_GGCC'C
Cuts at: 0 104 1002
Size: 104 898
ApaLI G'TGCA_C
Cuts at: 0 68 1002
Size: 68 934
AvaI C'yCGr_G
Cuts at: 0 163 1002
Size: 163 839
BanI G'GyrC_C
Cuts at: 0 361 509 1002
Size: 361 148 493
Fragments arranged by size:
493 361 148
BanII G_rGCy'C
Cuts at: 0 104 986 1002
Size: 104 882 16
Fragments arranged by size:
882 104 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 1002
Size: 476 526
BbvCI CC'TCA_GC
Cuts at: 0 58 176 1002
Size: 58 118 826
Fragments arranged by size:
826 118 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 188 1002
Size: 154 34 814
Fragments arranged by size:
814 154 34
BclI T'GATC_A
Cuts at: 0 620 1002
Size: 620 382
BfrBI ATG'CAT
Cuts at: 0 794 1002
Size: 794 208
BlpI GC'TnA_GC
Cuts at: 0 980 1002
Size: 980 22
Bme1580I G_kGCm'C
Cuts at: 0 72 104 1002
Size: 72 32 898
Fragments arranged by size:
898 72 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 719 1002
Size: 78 641 283
Fragments arranged by size:
641 283 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 910 1002
Size: 508 402 92
Fragments arranged by size:
508 402 92
Bpu10I CC'TnA_GC
Cuts at: 0 58 176 1002
Size: 58 118 826
Fragments arranged by size:
826 118 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 81 209 579 1002
Size: 81 128 370 423
Fragments arranged by size:
423 370 128 81
BsaWI w'CCGG_w
Cuts at: 0 200 1002
Size: 200 802
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 1002
Size: 178 30 794
Fragments arranged by size:
794 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 40 43 46 271 283 1002
Size: 34 3 3 3 3 225 12 719
Fragments arranged by size:
719 225 34 12 3 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 397 761 1002
Size: 4 393 364 241
Fragments arranged by size:
393 364 241 4
BsiHKAI G_wGCw'C
Cuts at: 0 72 986 1002
Size: 72 914 16
Fragments arranged by size:
914 72 16
Bsp1286I G_dGCh'C
Cuts at: 0 72 104 986 1002
Size: 72 32 882 16
Fragments arranged by size:
882 72 32 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 380 1002
Size: 380 622
BsrDI GCAATG_nn'
Cuts at: 0 782 1002
Size: 782 220
BstEII G'GTnAC_C
Cuts at: 0 606 1002
Size: 606 396
BtgI C'CryG_G
Cuts at: 0 122 611 1002
Size: 122 489 391
Fragments arranged by size:
489 391 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 969 1002
Size: 969 33
BtsI GCAGTG_nn'
Cuts at: 0 227 749 1002
Size: 227 522 253
Fragments arranged by size:
522 253 227
DraIII CAC_nnn'GTG
Cuts at: 0 530 1002
Size: 530 472
EaeI y'GGCC_r
Cuts at: 0 31 119 678 1002
Size: 31 88 559 324
Fragments arranged by size:
559 324 88 31
EarI CTCTTCn'nnn_
Cuts at: 0 604 1002
Size: 604 398
EcoICRI GAG'CTC
Cuts at: 0 984 1002
Size: 984 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 910 1002
Size: 508 402 92
Fragments arranged by size:
508 402 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 1002
Size: 568 434
EcoO109I rG'GnC_Cy
Cuts at: 0 100 392 537 1002
Size: 100 292 145 465
Fragments arranged by size:
465 292 145 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 955 1002
Size: 955 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 1002
Size: 481 32 489
Fragments arranged by size:
489 481 32
KpnI G_GTAC'C
Cuts at: 0 513 1002
Size: 513 489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 1002
Size: 108 894
MscI TGG'CCA
Cuts at: 0 121 1002
Size: 121 881
MslI CAynn'nnrTG
Cuts at: 0 187 292 454 616 1002
Size: 187 105 162 162 386
Fragments arranged by size:
386 187 162 162 105
MspA1I CmG'CkG
Cuts at: 0 10 371 1002
Size: 10 361 631
Fragments arranged by size:
631 361 10
NsiI A_TGCA'T
Cuts at: 0 796 1002
Size: 796 206
NspI r_CATG'y
Cuts at: 0 651 979 1002
Size: 651 328 23
Fragments arranged by size:
651 328 23
PciI A'CATG_T
Cuts at: 0 647 1002
Size: 647 355
PpuMI rG'GwC_Cy
Cuts at: 0 392 537 1002
Size: 392 145 465
Fragments arranged by size:
465 392 145
PspOMI G'GGCC_C
Cuts at: 0 100 1002
Size: 100 902
PvuII CAG'CTG
Cuts at: 0 10 1002
Size: 10 992
SacI G_AGCT'C
Cuts at: 0 986 1002
Size: 986 16
SanDI GG'GwC_CC
Cuts at: 0 537 1002
Size: 537 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 1002
Size: 178 32 792
Fragments arranged by size:
792 178 32
XbaI T'CTAG_A
Cuts at: 0 499 1002
Size: 499 503
XmnI GAAnn'nnTTC
Cuts at: 0 920 1002
Size: 920 82
Enzymes that do cut:
Acc65I AflIII AleI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BstEII BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I KpnI MmeI MscI MslI MspA1I NsiI NspI
PciI PpuMI PspOMI PvuII SacI SanDI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BglI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI Tth111I XcmI
XhoI XmaI ZraI