Restriction map for
pFN21AE6602
(Linear) (Six-base) MAPSORT of: KIEE6602.seq Check: 1214 from: 1 to: 714
KIEE6602.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AclI AA'CG_TT
Cuts at: 0 430 714
Size: 430 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 714
Size: 561 153
AleI CACnn'nnGTG
Cuts at: 0 530 714
Size: 530 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 714
Size: 301 32 381
Fragments arranged by size:
381 301 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 93 714
Size: 90 3 621
Fragments arranged by size:
621 90 3
ApaI G_GGCC'C
Cuts at: 0 126 573 714
Size: 126 447 141
Fragments arranged by size:
447 141 126
BanI G'GyrC_C
Cuts at: 0 374 610 714
Size: 374 236 104
Fragments arranged by size:
374 236 104
BanII G_rGCy'C
Cuts at: 0 104 126 573 714
Size: 104 22 447 141
Fragments arranged by size:
447 141 104 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 303 714
Size: 303 411
BglI GCCn_nnn'nGGC
Cuts at: 0 121 714
Size: 121 593
Bme1580I G_kGCm'C
Cuts at: 0 126 573 714
Size: 126 447 141
Fragments arranged by size:
447 141 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 274 313 714
Size: 33 241 39 401
Fragments arranged by size:
401 241 39 33
Bpu10I CC'TnA_GC
Cuts at: 0 22 714
Size: 22 692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 208 714
Size: 187 21 506
Fragments arranged by size:
506 187 21
BsaAI yAC'GTr
Cuts at: 0 600 714
Size: 600 114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 190 625 714
Size: 187 3 435 89
Fragments arranged by size:
435 187 89 3
BseYI C'CCAG_C
Cuts at: 0 83 103 566 714
Size: 83 20 463 148
Fragments arranged by size:
463 148 83 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 714
Size: 508 206
Bsp1286I G_dGCh'C
Cuts at: 0 104 126 573 714
Size: 104 22 447 141
Fragments arranged by size:
447 141 104 22
BspHI T'CATG_A
Cuts at: 0 548 714
Size: 548 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 632 714
Size: 632 82
BsrGI T'GTAC_A
Cuts at: 0 440 714
Size: 440 274
BstEII G'GTnAC_C
Cuts at: 0 78 714
Size: 78 636
BtsI GCAGTG_nn'
Cuts at: 0 525 714
Size: 525 189
EarI CTCTTCn'nnn_
Cuts at: 0 278 580 714
Size: 278 302 134
Fragments arranged by size:
302 278 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 714
Size: 163 551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 274 313 561 714
Size: 33 241 39 248 153
Fragments arranged by size:
248 241 153 39 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 331 714
Size: 202 129 383
Fragments arranged by size:
383 202 129
EcoO109I rG'GnC_Cy
Cuts at: 0 114 122 192 570 714
Size: 114 8 70 378 144
Fragments arranged by size:
378 144 114 70 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 714
Size: 258 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 714
Size: 490 32 192
Fragments arranged by size:
490 192 32
HindIII A'AGCT_T
Cuts at: 0 557 714
Size: 557 157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 714
Size: 77 637
MslI CAynn'nnrTG
Cuts at: 0 220 530 714
Size: 220 310 184
Fragments arranged by size:
310 220 184
MspA1I CmG'CkG
Cuts at: 0 90 566 714
Size: 90 476 148
Fragments arranged by size:
476 148 90
NspI r_CATG'y
Cuts at: 0 58 585 714
Size: 58 527 129
Fragments arranged by size:
527 129 58
PasI CC'CwG_GG
Cuts at: 0 119 714
Size: 119 595
PflMI CCAn_nnn'nTGG
Cuts at: 0 300 714
Size: 300 414
PfoI T'CCnGG_A
Cuts at: 0 209 714
Size: 209 505
PpuMI rG'GwC_Cy
Cuts at: 0 192 714
Size: 192 522
PspOMI G'GGCC_C
Cuts at: 0 122 569 714
Size: 122 447 145
Fragments arranged by size:
447 145 122
PstI C_TGCA'G
Cuts at: 0 629 713 714
Size: 629 84 1
Fragments arranged by size:
629 84 1
PvuII CAG'CTG
Cuts at: 0 90 714
Size: 90 624
ScaI AGT'ACT
Cuts at: 0 289 714
Size: 289 425
SfcI C'TryA_G
Cuts at: 0 272 625 709 714
Size: 272 353 84 5
Fragments arranged by size:
353 272 84 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 121 714
Size: 121 593
SmlI C'TyrA_G
Cuts at: 0 187 202 714
Size: 187 15 512
Fragments arranged by size:
512 187 15
SphI G_CATG'C
Cuts at: 0 585 714
Size: 585 129
StuI AGG'CCT
Cuts at: 0 676 714
Size: 676 38
StyI C'CwwG_G
Cuts at: 0 110 665 714
Size: 110 555 49
Fragments arranged by size:
555 110 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 714
Size: 392 322
TatI w'GTAC_w
Cuts at: 0 287 440 714
Size: 287 153 274
Fragments arranged by size:
287 274 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 714
Size: 367 32 315
Fragments arranged by size:
367 315 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 604 714
Size: 604 110
Enzymes that do cut:
AclI AcuI AleI AloI AlwNI ApaI BanI BanII
BciVI BglI Bme1580I BpmI Bpu10I BpuEI BsaAI BseRI
BseYI BsgI Bsp1286I BspHI BspMI BsrGI BstEII BtsI
EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII
MmeI MslI MspA1I NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII ScaI SfcI SfiI SmlI SphI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI