Restriction map for
pFN21AE6601
(Linear) (Six-base) MAPSORT of: KIEE6601.seq Check: 5372 from: 1 to: 705
KIEE6601.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 705
Size: 169 536
AatII G_ACGT'C
Cuts at: 0 431 705
Size: 431 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 705
Size: 448 257
AleI CACnn'nnGTG
Cuts at: 0 346 705
Size: 346 359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 705
Size: 40 665
AlwNI CAG_nnn'CTG
Cuts at: 0 105 117 147 705
Size: 105 12 30 558
Fragments arranged by size:
558 105 30 12
AvaI C'yCGr_G
Cuts at: 0 246 406 705
Size: 246 160 299
Fragments arranged by size:
299 246 160
BanI G'GyrC_C
Cuts at: 0 298 355 544 652 705
Size: 298 57 189 108 53
Fragments arranged by size:
298 189 108 57 53
BanII G_rGCy'C
Cuts at: 0 38 554 705
Size: 38 516 151
Fragments arranged by size:
516 151 38
BbeI G_GCGC'C
Cuts at: 0 302 359 548 656 705
Size: 302 57 189 108 49
Fragments arranged by size:
302 189 108 57 49
BglI GCCn_nnn'nGGC
Cuts at: 0 327 535 705
Size: 327 208 170
Fragments arranged by size:
327 208 170
BlpI GC'TnA_GC
Cuts at: 0 385 700 705
Size: 385 315 5
Fragments arranged by size:
385 315 5
Bme1580I G_kGCm'C
Cuts at: 0 512 705
Size: 512 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 469 705
Size: 310 159 236
Fragments arranged by size:
310 236 159
BsaHI Gr'CG_yC
Cuts at: 0 299 356 428 545 653 705
Size: 299 57 72 117 108 52
Fragments arranged by size:
299 117 108 72 57 52
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 351 570 705
Size: 25 326 219 135
Fragments arranged by size:
326 219 135 25
BseYI C'CCAG_C
Cuts at: 0 294 705
Size: 294 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 705
Size: 298 407
BsiEI CG_ry'CG
Cuts at: 0 192 331 587 705
Size: 192 139 256 118
Fragments arranged by size:
256 192 139 118
BsiHKAI G_wGCw'C
Cuts at: 0 391 554 705
Size: 391 163 151
Fragments arranged by size:
391 163 151
Bsp1286I G_dGCh'C
Cuts at: 0 38 391 512 554 705
Size: 38 353 121 42 151
Fragments arranged by size:
353 151 121 42 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 169 269 446 705
Size: 113 56 100 177 259
Fragments arranged by size:
259 177 113 100 56
BsrBI CCG'CTC
Cuts at: 0 228 705
Size: 228 477
BsrFI r'CCGG_y
Cuts at: 0 571 705
Size: 571 134
BssHII G'CGCG_C
Cuts at: 0 213 284 376 378 380 705
Size: 213 71 92 2 2 325
Fragments arranged by size:
325 213 92 71 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 117 705
Size: 105 12 588
Fragments arranged by size:
588 105 12
BtgI C'CryG_G
Cuts at: 0 322 560 705
Size: 322 238 145
Fragments arranged by size:
322 238 145
DraIII CAC_nnn'GTG
Cuts at: 0 69 705
Size: 69 636
DrdI GACnn_nn'nnGTC
Cuts at: 0 427 705
Size: 427 278
EaeI y'GGCC_r
Cuts at: 0 200 328 635 705
Size: 200 128 307 70
Fragments arranged by size:
307 200 128 70
EagI C'GGCC_G
Cuts at: 0 328 705
Size: 328 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 427 705
Size: 106 321 278
Fragments arranged by size:
321 278 106
EcoICRI GAG'CTC
Cuts at: 0 552 705
Size: 552 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 448 469 705
Size: 310 138 21 236
Fragments arranged by size:
310 236 138 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 705
Size: 109 596
EcoO109I rG'GnC_Cy
Cuts at: 0 195 459 630 705
Size: 195 264 171 75
Fragments arranged by size:
264 195 171 75
FspI TGC'GCA
Cuts at: 0 340 705
Size: 340 365
FspAI rTGC'GCAy
Cuts at: 0 340 705
Size: 340 365
HaeII r_GCGC'y
Cuts at: 0 25 302 359 548 572 656 705
Size: 25 277 57 189 24 84 49
Fragments arranged by size:
277 189 84 57 49 25 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 705
Size: 541 32 132
Fragments arranged by size:
541 132 32
HincII GTy'rAC
Cuts at: 0 504 705
Size: 504 201
KasI G'GCGC_C
Cuts at: 0 298 355 544 652 705
Size: 298 57 189 108 53
Fragments arranged by size:
298 189 108 57 53
MslI CAynn'nnrTG
Cuts at: 0 346 493 705
Size: 346 147 212
Fragments arranged by size:
346 212 147
MspA1I CmG'CkG
Cuts at: 0 312 324 333 515 705
Size: 312 12 9 182 190
Fragments arranged by size:
312 190 182 12 9
NaeI GCC'GGC
Cuts at: 0 573 705
Size: 573 132
NarI GG'CG_CC
Cuts at: 0 299 356 545 653 705
Size: 299 57 189 108 52
Fragments arranged by size:
299 189 108 57 52
NgoMIV G'CCGG_C
Cuts at: 0 571 705
Size: 571 134
NotI GC'GGCC_GC
Cuts at: 0 328 705
Size: 328 377
NspI r_CATG'y
Cuts at: 0 279 705
Size: 279 426
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 705
Size: 66 639
PpuMI rG'GwC_Cy
Cuts at: 0 459 705
Size: 459 246
PspXI vC'TCGA_Gb
Cuts at: 0 406 705
Size: 406 299
SacI G_AGCT'C
Cuts at: 0 554 705
Size: 554 151
SacII CC_GC'GG
Cuts at: 0 325 705
Size: 325 380
SexAI A'CCwGG_T
Cuts at: 0 65 705
Size: 65 640
SfoI GGC'GCC
Cuts at: 0 300 357 546 654 705
Size: 300 57 189 108 51
Fragments arranged by size:
300 189 108 57 51
SmlI C'TyrA_G
Cuts at: 0 406 705
Size: 406 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 573 705
Size: 225 348 132
Fragments arranged by size:
348 225 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 705
Size: 630 75
XhoI C'TCGA_G
Cuts at: 0 406 705
Size: 406 299
ZraI GAC'GTC
Cuts at: 0 429 705
Size: 429 276
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AlwNI AvaI BanI
BanII BbeI BglI BlpI Bme1580I BpmI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BtgI DraIII DrdI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I
HincII KasI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PflMI PpuMI PspXI SacI SacII SexAI SfoI
SmlI TaqII XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EarI
EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XmaI XmnI