(Linear) (Six-base) MAPSORT of: KIEE6596.seq  Check: 2683  from: 1  to: 192



KIEE6596.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     192 
   Size:        127      65
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     135     192 
   Size:        135      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      83      94     192 
   Size:         31      52      11      98

  Fragments arranged by size: 

                  98      52      31      11
 
BsrDI GCAATG_nn'

Cuts at:      0     131     192 
   Size:        131      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      83      94     127     192 
   Size:         31      52      11      33      65

  Fragments arranged by size: 

                  65      52      33      31      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     192 
   Size:         21     171
 
MslI CAynn'nnrTG

Cuts at:      0      88     192 
   Size:         88     104
 
SspI AAT'ATT

Cuts at:      0     149     192 
   Size:        149      43
 

 Enzymes that do cut:

     AcuI    BciVI     BpmI    BsrDI  Eco57MI     MmeI     MslI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI