(Linear) (Six-base) MAPSORT of: KIEE6594.seq  Check: 8060  from: 1  to: 216



KIEE6594.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

ApaI G_GGCC'C

Cuts at:      0      98     216 
   Size:         98     118
 
AvaI C'yCGr_G

Cuts at:      0      90     216 
   Size:         90     126
 
BanII G_rGCy'C

Cuts at:      0      98     216 
   Size:         98     118
 
Bme1580I G_kGCm'C

Cuts at:      0      98     216 
   Size:         98     118
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     216 
   Size:         98     118
 
BsrBI CCG'CTC

Cuts at:      0      73     216 
   Size:         73     143
 
BtgI C'CryG_G

Cuts at:      0     122     216 
   Size:        122      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0      94      95     216 
   Size:         94       1     121

  Fragments arranged by size: 

                 121      94       1
 
HaeII r_GCGC'y

Cuts at:      0      21     216 
   Size:         21     195
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     216 
   Size:        117      99
 
MspA1I CmG'CkG

Cuts at:      0     108     124     175     216 
   Size:        108      16      51      41

  Fragments arranged by size: 

                 108      51      41      16
 
NdeI CA'TA_TG

Cuts at:      0     180     216 
   Size:        180      36
 
PspOMI G'GGCC_C

Cuts at:      0      94     216 
   Size:         94     122
 
PstI C_TGCA'G

Cuts at:      0      68     216 
   Size:         68     148
 
PvuII CAG'CTG

Cuts at:      0     175     216 
   Size:        175      41
 
SacII CC_GC'GG

Cuts at:      0     125     216 
   Size:        125      91
 
SfcI C'TryA_G

Cuts at:      0      64     216 
   Size:         64     152
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     131     163     216 
   Size:        131      32      53

  Fragments arranged by size: 

                 131      53      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     202     216 
   Size:        202      14
 

 Enzymes that do cut:

     ApaI     AvaI    BanII Bme1580I Bsp1286I    BsrBI     BtgI EcoO109I
    HaeII     MmeI   MspA1I     NdeI   PspOMI     PstI    PvuII    SacII
     SfcI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI