(Linear) (Six-base) MAPSORT of: KIEE6593.seq  Check: 7945  from: 1  to: 153



KIEE6593.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     153 
   Size:        100      53
 
ApoI r'AATT_y

Cuts at:      0      88     153 
   Size:         88      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     115     153 
   Size:         88      27      38

  Fragments arranged by size: 

                  88      38      27
 
BglII A'GATC_T

Cuts at:      0      50     153 
   Size:         50     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     153 
   Size:         33     120
 
BstYI r'GATC_y

Cuts at:      0      50     153 
   Size:         50     103
 
BtsI GCAGTG_nn'

Cuts at:      0      71     153 
   Size:         71      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     100     153 
   Size:         33      67      53

  Fragments arranged by size: 

                  67      53      33
 
EcoRI G'AATT_C

Cuts at:      0      88     153 
   Size:         88      65
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BglII     BpmI    BstYI     BtsI  Eco57MI
    EcoRI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI