Restriction map for
pFN21AE6592
(Linear) (Six-base) MAPSORT of: KIEE6592.seq Check: 2533 from: 1 to: 606
KIEE6592.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 392 606
Size: 392 214
AfeI AGC'GCT
Cuts at: 0 85 606
Size: 85 521
ApaI G_GGCC'C
Cuts at: 0 136 606
Size: 136 470
AvaI C'yCGr_G
Cuts at: 0 591 606
Size: 591 15
BanI G'GyrC_C
Cuts at: 0 51 300 606
Size: 51 249 306
Fragments arranged by size:
306 249 51
BanII G_rGCy'C
Cuts at: 0 136 606
Size: 136 470
BbeI G_GCGC'C
Cuts at: 0 304 606
Size: 304 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 104 219 253 606
Size: 70 34 115 34 353
Fragments arranged by size:
353 115 70 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 404 424 451 606
Size: 404 20 27 155
Fragments arranged by size:
404 155 27 20
Bme1580I G_kGCm'C
Cuts at: 0 136 286 606
Size: 136 150 320
Fragments arranged by size:
320 150 136
BmgBI CAC'GTC
Cuts at: 0 369 414 606
Size: 369 45 192
Fragments arranged by size:
369 192 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 606
Size: 207 399
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 606
Size: 178 428
BsaHI Gr'CG_yC
Cuts at: 0 4 301 606
Size: 4 297 305
Fragments arranged by size:
305 297 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 606
Size: 100 30 476
Fragments arranged by size:
476 100 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 121 124 286 304 606
Size: 118 3 3 162 18 302
Fragments arranged by size:
302 162 118 18 3 3
BseYI C'CCAG_C
Cuts at: 0 169 606
Size: 169 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 422 606
Size: 274 148 184
Fragments arranged by size:
274 184 148
BsiEI CG_ry'CG
Cuts at: 0 68 147 224 377 606
Size: 68 79 77 153 229
Fragments arranged by size:
229 153 79 77 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 37 64 606
Size: 37 27 542
Fragments arranged by size:
542 37 27
Bsp1286I G_dGCh'C
Cuts at: 0 136 286 606
Size: 136 150 320
Fragments arranged by size:
320 150 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 606
Size: 245 361
BsrBI CCG'CTC
Cuts at: 0 386 606
Size: 386 220
BssHII G'CGCG_C
Cuts at: 0 313 487 606
Size: 313 174 119
Fragments arranged by size:
313 174 119
BssSI C'ACGA_G
Cuts at: 0 247 606
Size: 247 359
BtgI C'CryG_G
Cuts at: 0 152 179 558 598 606
Size: 152 27 379 40 8
Fragments arranged by size:
379 152 40 27 8
DraIII CAC_nnn'GTG
Cuts at: 0 369 477 606
Size: 369 108 129
Fragments arranged by size:
369 129 108
EaeI y'GGCC_r
Cuts at: 0 144 221 374 416 509 606
Size: 144 77 153 42 93 97
Fragments arranged by size:
153 144 97 93 77 42
EagI C'GGCC_G
Cuts at: 0 144 221 374 606
Size: 144 77 153 232
Fragments arranged by size:
232 153 144 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 283 355 606
Size: 36 247 72 251
Fragments arranged by size:
251 247 72 36
EcoO109I rG'GnC_Cy
Cuts at: 0 26 124 234 294 495 606
Size: 26 98 110 60 201 111
Fragments arranged by size:
201 111 110 98 60 26
HaeII r_GCGC'y
Cuts at: 0 87 304 606
Size: 87 217 302
Fragments arranged by size:
302 217 87
KasI G'GCGC_C
Cuts at: 0 300 606
Size: 300 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 606
Size: 80 526
MspA1I CmG'CkG
Cuts at: 0 181 258 560 600 606
Size: 181 77 302 40 6
Fragments arranged by size:
302 181 77 40 6
NarI GG'CG_CC
Cuts at: 0 301 606
Size: 301 305
NotI GC'GGCC_GC
Cuts at: 0 144 374 606
Size: 144 230 232
Fragments arranged by size:
232 230 144
PasI CC'CwG_GG
Cuts at: 0 9 606
Size: 9 597
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 606
Size: 369 237
PpuMI rG'GwC_Cy
Cuts at: 0 124 234 495 606
Size: 124 110 261 111
Fragments arranged by size:
261 124 111 110
PspOMI G'GGCC_C
Cuts at: 0 132 606
Size: 132 474
SacII CC_GC'GG
Cuts at: 0 182 561 601 606
Size: 182 379 40 5
Fragments arranged by size:
379 182 40 5
SfoI GGC'GCC
Cuts at: 0 302 606
Size: 302 304
SmaI CCC'GGG
Cuts at: 0 593 606
Size: 593 13
StuI AGG'CCT
Cuts at: 0 277 606
Size: 277 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 202 589 606
Size: 82 120 387 17
Fragments arranged by size:
387 120 82 17
XmaI C'CCGG_G
Cuts at: 0 591 606
Size: 591 15
Enzymes that do cut:
AccI AfeI ApaI AvaI BanI BanII BbeI BcgI
BglI Bme1580I BmgBI BplI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BssHII
BssSI BtgI DraIII EaeI EagI EciI EcoO109I HaeII
KasI MmeI MspA1I NarI NotI PasI PflMI PpuMI
PspOMI SacII SfoI SmaI StuI TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI