(Linear) (Six-base) MAPSORT of: KIEE6590.seq  Check: 9934  from: 1  to: 543



KIEE6590.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     543 
   Size:         97     446
 
AgeI A'CCGG_T

Cuts at:      0     332     543 
   Size:        332     211
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     310     342     543 
   Size:        310      32     201

  Fragments arranged by size: 

                 310     201      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     286     486     543 
   Size:        286     200      57

  Fragments arranged by size: 

                 286     200      57
 
BanI G'GyrC_C

Cuts at:      0     463     543 
   Size:        463      80
 
BbvCI CC'TCA_GC

Cuts at:      0      27     401     454     543 
   Size:         27     374      53      89

  Fragments arranged by size: 

                 374      89      53      27
 
Bme1580I G_kGCm'C

Cuts at:      0     156     468     543 
   Size:        156     312      75

  Fragments arranged by size: 

                 312     156      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      42      72     543 
   Size:         42      30     471

  Fragments arranged by size: 

                 471      42      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     502     543 
   Size:        322     180      41

  Fragments arranged by size: 

                 322     180      41
 
Bpu10I CC'TnA_GC

Cuts at:      0      27     401     454     543 
   Size:         27     374      53      89

  Fragments arranged by size: 

                 374      89      53      27
 
BsaWI w'CCGG_w

Cuts at:      0     332     543 
   Size:        332     211
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     499     543 
   Size:        344     155      44

  Fragments arranged by size: 

                 344     155      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     468     543 
   Size:        156     312      75

  Fragments arranged by size: 

                 312     156      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     278     543 
   Size:        278     265
 
BsrBI CCG'CTC

Cuts at:      0     528     543 
   Size:        528      15
 
BsrDI GCAATG_nn'

Cuts at:      0     135     543 
   Size:        135     408
 
BsrFI r'CCGG_y

Cuts at:      0     332     543 
   Size:        332     211
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     486     543 
   Size:        486      57
 
BstBI TT'CG_AA

Cuts at:      0     159     543 
   Size:        159     384
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     300     543 
   Size:        300     243
 
EarI CTCTTCn'nnn_

Cuts at:      0     243     543 
   Size:        243     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     322     502     543 
   Size:         97     225     180      41

  Fragments arranged by size: 

                 225     180      97      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     278     543 
   Size:        278     265
 
EcoO109I rG'GnC_Cy

Cuts at:      0     267     543 
   Size:        267     276
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     488     520     543 
   Size:        488      32      23

  Fragments arranged by size: 

                 488      32      23
 
HincII GTy'rAC

Cuts at:      0     102     543 
   Size:        102     441
 
HpaI GTT'AAC

Cuts at:      0     102     543 
   Size:        102     441
 
MslI CAynn'nnrTG

Cuts at:      0     109     543 
   Size:        109     434
 
MspA1I CmG'CkG

Cuts at:      0     400     543 
   Size:        400     143
 
PfoI T'CCnGG_A

Cuts at:      0      88     506     543 
   Size:         88     418      37

  Fragments arranged by size: 

                 418      88      37
 
PpuMI rG'GwC_Cy

Cuts at:      0     267     543 
   Size:        267     276
 
PstI C_TGCA'G

Cuts at:      0     491     543 
   Size:        491      52
 
SfcI C'TryA_G

Cuts at:      0     487     543 
   Size:        487      56
 
StuI AGG'CCT

Cuts at:      0     184     543 
   Size:        184     359
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     297     444     543 
   Size:        297     147      99

  Fragments arranged by size: 

                 297     147      99
 

 Enzymes that do cut:

     AcuI     AgeI     AloI    AlwNI     BanI    BbvCI Bme1580I     BplI
     BpmI   Bpu10I    BsaWI     BsgI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI    BstBI    BstXI     EarI  Eco57MI    EcoNI EcoO109I
    Hin4I   HincII     HpaI     MslI   MspA1I     PfoI    PpuMI     PstI
     SfcI     StuI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI   BssHII    BssSI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI