Restriction map for
pFN21AE6589
(Linear) (Six-base) MAPSORT of: KIEE6589.seq Check: 3925 from: 1 to: 534
KIEE6589.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AfeI AGC'GCT
Cuts at: 0 367 534
Size: 367 167
AflIII A'CryG_T
Cuts at: 0 195 419 534
Size: 195 224 115
Fragments arranged by size:
224 195 115
ApaLI G'TGCA_C
Cuts at: 0 43 534
Size: 43 491
ApoI r'AATT_y
Cuts at: 0 223 534
Size: 223 311
AvaI C'yCGr_G
Cuts at: 0 9 275 534
Size: 9 266 259
Fragments arranged by size:
266 259 9
AvrII C'CTAG_G
Cuts at: 0 126 534
Size: 126 408
BanI G'GyrC_C
Cuts at: 0 492 534
Size: 492 42
BanII G_rGCy'C
Cuts at: 0 345 534
Size: 345 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 534
Size: 254 280
BciVI GTATCCnnnnn_n'
Cuts at: 0 147 534
Size: 147 387
Bme1580I G_kGCm'C
Cuts at: 0 47 427 534
Size: 47 380 107
Fragments arranged by size:
380 107 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 230 534
Size: 230 304
BsaI GGTCTCn'nnnn_
Cuts at: 0 116 534
Size: 116 418
BsaAI yAC'GTr
Cuts at: 0 196 422 534
Size: 196 226 112
Fragments arranged by size:
226 196 112
BsaWI w'CCGG_w
Cuts at: 0 247 534
Size: 247 287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 534
Size: 180 30 324
Fragments arranged by size:
324 180 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 490 534
Size: 134 356 44
Fragments arranged by size:
356 134 44
BseYI C'CCAG_C
Cuts at: 0 100 534
Size: 100 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 534
Size: 315 219
BsiEI CG_ry'CG
Cuts at: 0 36 534
Size: 36 498
BsiHKAI G_wGCw'C
Cuts at: 0 47 285 534
Size: 47 238 249
Fragments arranged by size:
249 238 47
Bsp1286I G_dGCh'C
Cuts at: 0 47 285 345 427 534
Size: 47 238 60 82 107
Fragments arranged by size:
238 107 82 60 47
BspEI T'CCGG_A
Cuts at: 0 247 534
Size: 247 287
BssHII G'CGCG_C
Cuts at: 0 25 534
Size: 25 509
BstEII G'GTnAC_C
Cuts at: 0 55 534
Size: 55 479
BtgI C'CryG_G
Cuts at: 0 326 534
Size: 326 208
EaeI y'GGCC_r
Cuts at: 0 33 534
Size: 33 501
EagI C'GGCC_G
Cuts at: 0 33 534
Size: 33 501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 534
Size: 346 188
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 358 534
Size: 294 64 176
Fragments arranged by size:
294 176 64
HaeII r_GCGC'y
Cuts at: 0 369 384 534
Size: 369 15 150
Fragments arranged by size:
369 150 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 534
Size: 150 384
MspA1I CmG'CkG
Cuts at: 0 303 328 534
Size: 303 25 206
Fragments arranged by size:
303 206 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 107 534
Size: 107 427
PmlI CAC'GTG
Cuts at: 0 196 422 534
Size: 196 226 112
Fragments arranged by size:
226 196 112
PstI C_TGCA'G
Cuts at: 0 98 534
Size: 98 436
RsrII CG'GwC_CG
Cuts at: 0 38 534
Size: 38 496
SacII CC_GC'GG
Cuts at: 0 329 534
Size: 329 205
SfcI C'TryA_G
Cuts at: 0 94 534
Size: 94 440
SmaI CCC'GGG
Cuts at: 0 11 534
Size: 11 523
StyI C'CwwG_G
Cuts at: 0 126 534
Size: 126 408
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 422 534
Size: 422 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 180 212 534
Size: 180 32 322
Fragments arranged by size:
322 180 32
XmaI C'CCGG_G
Cuts at: 0 9 534
Size: 9 525
Enzymes that do cut:
AfeI AflIII ApaLI ApoI AvaI AvrII BanI BanII
BbsI BciVI Bme1580I BmrI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BssHII
BstEII BtgI EaeI EagI EciI EcoNI HaeII MmeI
MspA1I PflMI PmlI PstI RsrII SacII SfcI SmaI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaBI BsaHI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI