(Linear) (Six-base) MAPSORT of: KIEE6588.seq  Check: 2754  from: 1  to: 531



KIEE6588.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

Acc65I G'GTAC_C

Cuts at:      0     379     531 
   Size:        379     152
 
AgeI A'CCGG_T

Cuts at:      0     184     531 
   Size:        184     347
 
AlwNI CAG_nnn'CTG

Cuts at:      0      23      71     101     131     161     269     531 
   Size:         23      48      30      30      30     108     262

  Fragments arranged by size: 

                 262     108      48      30      30      30      23
 
BanI G'GyrC_C

Cuts at:      0     353     379     531 
   Size:        353      26     152

  Fragments arranged by size: 

                 353     152      26
 
BanII G_rGCy'C

Cuts at:      0     524     531 
   Size:        524       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     317     531 
   Size:        317     214
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     490     531 
   Size:        490      41
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     114     531 
   Size:        114     417
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     531 
   Size:         48     483
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      85     115     531 
   Size:         85      30     416

  Fragments arranged by size: 

                 416      85      30
 
BsaWI w'CCGG_w

Cuts at:      0     184     531 
   Size:        184     347
 
BseYI C'CCAG_C

Cuts at:      0      34     531 
   Size:         34     497
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396     421     531 
   Size:        396      25     110

  Fragments arranged by size: 

                 396     110      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     340     531 
   Size:        340     191
 
Bsp1286I G_dGCh'C

Cuts at:      0     340     524     531 
   Size:        340     184       7

  Fragments arranged by size: 

                 340     184       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64     391     531 
   Size:         64     327     140

  Fragments arranged by size: 

                 327     140      64
 
BsrFI r'CCGG_y

Cuts at:      0     184     531 
   Size:        184     347
 
BtsI GCAGTG_nn'

Cuts at:      0     330     531 
   Size:        330     201
 
EaeI y'GGCC_r

Cuts at:      0     309     531 
   Size:        309     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     531 
   Size:         48     483
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     531 
   Size:         53     478
 
KpnI G_GTAC'C

Cuts at:      0     383     531 
   Size:        383     148
 
MscI TGG'CCA

Cuts at:      0     311     531 
   Size:        311     220
 
MspA1I CmG'CkG

Cuts at:      0      38      68     128     435     531 
   Size:         38      30      60     307      96

  Fragments arranged by size: 

                 307      96      60      38      30
 
PpuMI rG'GwC_Cy

Cuts at:      0      53     531 
   Size:         53     478
 
PvuII CAG'CTG

Cuts at:      0      38      68     128     435     531 
   Size:         38      30      60     307      96

  Fragments arranged by size: 

                 307      96      60      38      30
 
StuI AGG'CCT

Cuts at:      0     451     531 
   Size:        451      80
 

 Enzymes that do cut:

   Acc65I     AgeI    AlwNI     BanI    BanII     BglI     BmrI     BplI
     BpmI     BsaI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrFI     BtsI     EaeI  Eco57MI EcoO109I     KpnI     MscI   MspA1I
    PpuMI    PvuII     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI