Restriction map for
pFN21AE6587
(Linear) (Six-base) MAPSORT of: KIEE6587.seq Check: 4339 from: 1 to: 525
KIEE6587.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 106 306 525
Size: 106 200 219
Fragments arranged by size:
219 200 106
AvaI C'yCGr_G
Cuts at: 0 215 525
Size: 215 310
BanI G'GyrC_C
Cuts at: 0 185 525
Size: 185 340
BanII G_rGCy'C
Cuts at: 0 268 293 518 525
Size: 268 25 225 7
Fragments arranged by size:
268 225 25 7
BbeI G_GCGC'C
Cuts at: 0 189 525
Size: 189 336
BglI GCCn_nnn'nGGC
Cuts at: 0 72 525
Size: 72 453
BlpI GC'TnA_GC
Cuts at: 0 52 97 525
Size: 52 45 428
Fragments arranged by size:
428 52 45
Bme1580I G_kGCm'C
Cuts at: 0 357 525
Size: 357 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 525
Size: 307 218
Bpu10I CC'TnA_GC
Cuts at: 0 493 525
Size: 493 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 493 525
Size: 252 241 32
Fragments arranged by size:
252 241 32
BsaHI Gr'CG_yC
Cuts at: 0 186 525
Size: 186 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 59 89 525
Size: 59 30 436
Fragments arranged by size:
436 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 525
Size: 145 380
BseYI C'CCAG_C
Cuts at: 0 517 525
Size: 517 8
BsiHKAI G_wGCw'C
Cuts at: 0 268 525
Size: 268 257
Bsp1286I G_dGCh'C
Cuts at: 0 268 293 357 518 525
Size: 268 25 64 161 7
Fragments arranged by size:
268 161 64 25 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 106 306 525
Size: 106 200 219
Fragments arranged by size:
219 200 106
BsrFI r'CCGG_y
Cuts at: 0 182 525
Size: 182 343
BssSI C'ACGA_G
Cuts at: 0 127 525
Size: 127 398
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 285 525
Size: 228 57 240
Fragments arranged by size:
240 228 57
BtgI C'CryG_G
Cuts at: 0 67 525
Size: 67 458
EaeI y'GGCC_r
Cuts at: 0 64 180 525
Size: 64 116 345
Fragments arranged by size:
345 116 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 27 525
Size: 27 498
EcoICRI GAG'CTC
Cuts at: 0 266 525
Size: 266 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 525
Size: 307 218
EcoO109I rG'GnC_Cy
Cuts at: 0 378 525
Size: 378 147
HaeII r_GCGC'y
Cuts at: 0 189 340 525
Size: 189 151 185
Fragments arranged by size:
189 185 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 525
Size: 137 32 356
Fragments arranged by size:
356 137 32
KasI G'GCGC_C
Cuts at: 0 185 525
Size: 185 340
MspA1I CmG'CkG
Cuts at: 0 51 525
Size: 51 474
NaeI GCC'GGC
Cuts at: 0 184 525
Size: 184 341
NarI GG'CG_CC
Cuts at: 0 186 525
Size: 186 339
NgoMIV G'CCGG_C
Cuts at: 0 182 525
Size: 182 343
PfoI T'CCnGG_A
Cuts at: 0 155 284 525
Size: 155 129 241
Fragments arranged by size:
241 155 129
PstI C_TGCA'G
Cuts at: 0 488 525
Size: 488 37
PvuII CAG'CTG
Cuts at: 0 51 525
Size: 51 474
SacI G_AGCT'C
Cuts at: 0 268 525
Size: 268 257
SfcI C'TryA_G
Cuts at: 0 484 525
Size: 484 41
SfoI GGC'GCC
Cuts at: 0 187 525
Size: 187 338
SmaI CCC'GGG
Cuts at: 0 217 525
Size: 217 308
SmlI C'TyrA_G
Cuts at: 0 267 472 525
Size: 267 205 53
Fragments arranged by size:
267 205 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 525
Size: 252 273
TatI w'GTAC_w
Cuts at: 0 134 525
Size: 134 391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 525
Size: 59 32 434
Fragments arranged by size:
434 59 32
XmaI C'CCGG_G
Cuts at: 0 215 525
Size: 215 310
Enzymes that do cut:
AarI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstXI BtgI EaeI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MspA1I NaeI
NarI NgoMIV PfoI PstI PvuII SacI SfcI SfoI
SmaI SmlI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI