(Linear) (Six-base) MAPSORT of: KIEE6585.seq  Check: 2495  from: 1  to: 477



KIEE6585.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      58      85     172     187     421     477 
   Size:         58      27      87      15     234      56

  Fragments arranged by size: 

                 234      87      58      56      27      15
 
AleI CACnn'nnGTG

Cuts at:      0      67     445     477 
   Size:         67     378      32

  Fragments arranged by size: 

                 378      67      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     125     209     284     458     477 
   Size:        125      84      75     174      19

  Fragments arranged by size: 

                 174     125      84      75      19
 
ApaLI G'TGCA_C

Cuts at:      0     287     477 
   Size:        287     190
 
Bme1580I G_kGCm'C

Cuts at:      0     291     477 
   Size:        291     186
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     400     477 
   Size:        400      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     477 
   Size:         28     449
 
BseYI C'CCAG_C

Cuts at:      0     393     477 
   Size:        393      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     291     477 
   Size:        291     186
 
Bsp1286I G_dGCh'C

Cuts at:      0     291     477 
   Size:        291     186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      43      58      85     172     187     319     421     477 
   Size:         43      15      27      87      15     132     102      56

  Fragments arranged by size: 

                 132     102      87      56      43      27      15      15
            
 
BsrGI T'GTAC_A

Cuts at:      0     449     477 
   Size:        449      28
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      92     236     477 
   Size:         92     144     241

  Fragments arranged by size: 

                 241     144      92
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     361     477 
   Size:        361     116
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     130     165     477 
   Size:        130      35     312

  Fragments arranged by size: 

                 312     130      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      39     168     417     477 
   Size:         39     129     249      60

  Fragments arranged by size: 

                 249     129      60      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     477 
   Size:        392      85
 
MslI CAynn'nnrTG

Cuts at:      0      67     196     445     477 
   Size:         67     129     249      32

  Fragments arranged by size: 

                 249     129      67      32
 
MspA1I CmG'CkG

Cuts at:      0     122     206     266     455     477 
   Size:        122      84      60     189      22

  Fragments arranged by size: 

                 189     122      84      60      22
 
NspI r_CATG'y

Cuts at:      0     254     477 
   Size:        254     223
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      53     477 
   Size:         53     424
 
PstI C_TGCA'G

Cuts at:      0      43      82     304     421     477 
   Size:         43      39     222     117      56

  Fragments arranged by size: 

                 222     117      56      43      39
 
PvuII CAG'CTG

Cuts at:      0     122     206     266     455     477 
   Size:        122      84      60     189      22

  Fragments arranged by size: 

                 189     122      84      60      22
 
SfcI C'TryA_G

Cuts at:      0      39      78     168     225     300     417     477 
   Size:         39      39      90      57      75     117      60

  Fragments arranged by size: 

                 117      90      75      60      57      39      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     158     477 
   Size:        158     319
 
TatI w'GTAC_w

Cuts at:      0     296     449     477 
   Size:        296     153      28

  Fragments arranged by size: 

                 296     153      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      30     477 
   Size:         30     447
 

 Enzymes that do cut:

     AarI     AleI    AlwNI    ApaLI Bme1580I     BsaI    BsaXI    BseYI
  BsiHKAI Bsp1286I    BspMI    BsrGI   BstAPI    BstXI    CspCI    EcoNI
     MmeI     MslI   MspA1I     NspI    PflMI     PstI    PvuII     SfcI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI