(Linear) (Six-base) MAPSORT of: KIEE6579.seq  Check: 8132  from: 1  to: 432



KIEE6579.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     352     432 
   Size:        352      80
 
ApaI G_GGCC'C

Cuts at:      0     323     432 
   Size:        323     109
 
AvrII C'CTAG_G

Cuts at:      0     295     432 
   Size:        295     137
 
BamHI G'GATC_C

Cuts at:      0      24     432 
   Size:         24     408
 
BanI G'GyrC_C

Cuts at:      0     218     413     432 
   Size:        218     195      19

  Fragments arranged by size: 

                 218     195      19
 
BanII G_rGCy'C

Cuts at:      0     284     323     432 
   Size:        284      39     109

  Fragments arranged by size: 

                 284     109      39
 
BglII A'GATC_T

Cuts at:      0     376     432 
   Size:        376      56
 
Bme1580I G_kGCm'C

Cuts at:      0     323     432 
   Size:        323     109
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     432 
   Size:        374      58
 
Bpu10I CC'TnA_GC

Cuts at:      0     283     432 
   Size:        283     149
 
BsaWI w'CCGG_w

Cuts at:      0      66     432 
   Size:         66     366
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     432 
   Size:         22     410
 
BseYI C'CCAG_C

Cuts at:      0     345     432 
   Size:        345      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     432 
   Size:        172     260
 
BsiHKAI G_wGCw'C

Cuts at:      0     284     432 
   Size:        284     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     284     323     432 
   Size:        284      39     109

  Fragments arranged by size: 

                 284     109      39
 
BsrBI CCG'CTC

Cuts at:      0     165     432 
   Size:        165     267
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     146     432 
   Size:        146     286
 
BstYI r'GATC_y

Cuts at:      0      24     135     376     432 
   Size:         24     111     241      56

  Fragments arranged by size: 

                 241     111      56      24
 
BtgI C'CryG_G

Cuts at:      0     116     417     432 
   Size:        116     301      15

  Fragments arranged by size: 

                 301     116      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     272     432 
   Size:        272     160
 
EcoICRI GAG'CTC

Cuts at:      0     282     432 
   Size:        282     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     432 
   Size:        374      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     199     313     320     432 
   Size:        199     114       7     112

  Fragments arranged by size: 

                 199     114     112       7
 
NcoI C'CATG_G

Cuts at:      0     417     432 
   Size:        417      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     352     432 
   Size:        352      80
 
PfoI T'CCnGG_A

Cuts at:      0     351     432 
   Size:        351      81
 
PspOMI G'GGCC_C

Cuts at:      0     319     432 
   Size:        319     113
 
SacI G_AGCT'C

Cuts at:      0     284     432 
   Size:        284     148
 
SapI GCTCTTCn'nnn_

Cuts at:      0     272     432 
   Size:        272     160
 
SspI AAT'ATT

Cuts at:      0     171     432 
   Size:        171     261
 
StuI AGG'CCT

Cuts at:      0     288     432 
   Size:        288     144
 
StyI C'CwwG_G

Cuts at:      0     139     272     295     417     432 
   Size:        139     133      23     122      15

  Fragments arranged by size: 

                 139     133     122      23      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     138     432 
   Size:        138     294
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       9      41     432 
   Size:          9      32     391

  Fragments arranged by size: 

                 391      32       9
 

 Enzymes that do cut:

    AlwNI     ApaI    AvrII    BamHI     BanI    BanII    BglII Bme1580I
     BpmI   Bpu10I    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrBI    BstXI    BstYI     BtgI     EarI  EcoICRI  Eco57MI EcoO109I
     NcoI    PflMI     PfoI   PspOMI     SacI     SapI     SspI     StuI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI