Restriction map for
pFN21AE6569
(Linear) (Six-base) MAPSORT of: KIEE6569.seq Check: 4540 from: 1 to: 639
KIEE6569.orf
With 182 enzymes: SgfI *
December 25, 2014 17:19 ..
AflIII A'CryG_T
Cuts at: 0 221 639
Size: 221 418
AlwNI CAG_nnn'CTG
Cuts at: 0 438 639
Size: 438 201
ApaI G_GGCC'C
Cuts at: 0 389 639
Size: 389 250
AscI GG'CGCG_CC
Cuts at: 0 334 639
Size: 334 305
AvaI C'yCGr_G
Cuts at: 0 112 130 639
Size: 112 18 509
Fragments arranged by size:
509 112 18
BanI G'GyrC_C
Cuts at: 0 456 639
Size: 456 183
BanII G_rGCy'C
Cuts at: 0 119 389 536 639
Size: 119 270 147 103
Fragments arranged by size:
270 147 119 103
BbeI G_GCGC'C
Cuts at: 0 460 639
Size: 460 179
BglI GCCn_nnn'nGGC
Cuts at: 0 326 616 639
Size: 326 290 23
Fragments arranged by size:
326 290 23
Bme1580I G_kGCm'C
Cuts at: 0 389 639
Size: 389 250
BmgBI CAC'GTC
Cuts at: 0 222 639
Size: 222 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 639
Size: 169 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 639
Size: 520 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 639
Size: 205 434
BsaAI yAC'GTr
Cuts at: 0 264 639
Size: 264 375
BsaHI Gr'CG_yC
Cuts at: 0 457 639
Size: 457 182
BsaWI w'CCGG_w
Cuts at: 0 141 639
Size: 141 498
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 451 639
Size: 232 219 188
Fragments arranged by size:
232 219 188
BsiEI CG_ry'CG
Cuts at: 0 360 411 565 639
Size: 360 51 154 74
Fragments arranged by size:
360 154 74 51
BsiHKAI G_wGCw'C
Cuts at: 0 119 316 536 639
Size: 119 197 220 103
Fragments arranged by size:
220 197 119 103
Bsp1286I G_dGCh'C
Cuts at: 0 119 316 389 536 639
Size: 119 197 73 147 103
Fragments arranged by size:
197 147 119 103 73
BsrBI CCG'CTC
Cuts at: 0 559 639
Size: 559 80
BsrFI r'CCGG_y
Cuts at: 0 294 639
Size: 294 345
BssHII G'CGCG_C
Cuts at: 0 334 419 462 464 466 639
Size: 334 85 43 2 2 173
Fragments arranged by size:
334 173 85 43 2 2
BssSI C'ACGA_G
Cuts at: 0 489 639
Size: 489 150
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 639
Size: 429 210
BstEII G'GTnAC_C
Cuts at: 0 297 639
Size: 297 342
BtgI C'CryG_G
Cuts at: 0 98 360 583 639
Size: 98 262 223 56
Fragments arranged by size:
262 223 98 56
EaeI y'GGCC_r
Cuts at: 0 318 357 562 639
Size: 318 39 205 77
Fragments arranged by size:
318 205 77 39
EagI C'GGCC_G
Cuts at: 0 357 562 639
Size: 357 205 77
Fragments arranged by size:
357 205 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 190 639
Size: 190 449
EcoICRI GAG'CTC
Cuts at: 0 117 534 639
Size: 117 417 105
Fragments arranged by size:
417 117 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 639
Size: 169 470
HaeII r_GCGC'y
Cuts at: 0 290 460 507 605 639
Size: 290 170 47 98 34
Fragments arranged by size:
290 170 98 47 34
KasI G'GCGC_C
Cuts at: 0 456 639
Size: 456 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 639
Size: 509 130
MspA1I CmG'CkG
Cuts at: 0 236 362 398 435 585 639
Size: 236 126 36 37 150 54
Fragments arranged by size:
236 150 126 54 37 36
NarI GG'CG_CC
Cuts at: 0 457 639
Size: 457 182
PspOMI G'GGCC_C
Cuts at: 0 385 639
Size: 385 254
PvuII CAG'CTG
Cuts at: 0 236 435 639
Size: 236 199 204
Fragments arranged by size:
236 204 199
SacI G_AGCT'C
Cuts at: 0 119 536 639
Size: 119 417 103
Fragments arranged by size:
417 119 103
SacII CC_GC'GG
Cuts at: 0 363 586 639
Size: 363 223 53
Fragments arranged by size:
363 223 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 326 639
Size: 326 313
SfoI GGC'GCC
Cuts at: 0 458 639
Size: 458 181
SmlI C'TyrA_G
Cuts at: 0 535 639
Size: 535 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 565 639
Size: 283 282 74
Fragments arranged by size:
283 282 74
TatI w'GTAC_w
Cuts at: 0 230 639
Size: 230 409
Enzymes that do cut:
AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI
BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII
BssSI BstAPI BstEII BtgI EaeI EagI EciI EcoICRI
Eco57MI HaeII KasI MmeI MspA1I NarI PspOMI PvuII
SacI SacII SfiI SfoI SmlI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BsaBI BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI